Rumen fermentation, intramuscular fat fatty acid profiles and related rumen bacterial populations of Holstein bulls fed diets with different energy levels

Wang, Haibo; He, Yang; Li, Hang; Wu, Fei; Qiu, Qinghua; Niu, Wen-jing; Gao, Zhibiao; Su, Huawei; Cao, Binghai

doi:10.1007/s00253-019-09839-3

Cited by 73 publications

(87 citation statements)

References 55 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Similar to the results of decreased bacterial richness in Group H, the high energy and protein levels of diets reduced the complexity of rumen bacterial interactions. Among the genera detected in the present study, Prevotella_1, belonging to the Bacteroidetes, was the most abundant bacteria in both the rumens of goats fed with different diets, which was consistent with the previous reports [3,5]. In addition, the population of Prevotella_1 was signi cantly increased in Group H. The difference of abundances between the groups might be related to bacterial functions.…”

Section: Comparison Of the Composition And Differences Of Ruminal Bacsupporting

confidence: 92%

“…In addition, the population of Prevotella_1 was signi cantly increased in Group H. The difference of abundances between the groups might be related to bacterial functions. The genus Prevotella is mainly involved in protein metabolism [3,5,49]. Wang et al [3] reported that the number of Prevotella_1 was increased when the animals fed with the high protein diet.…”

Section: Comparison Of the Composition And Differences Of Ruminal Bacmentioning

confidence: 99%

“…Traditional grazing management is mainly dependent on natural pastures, which are limited in the extremely harsh winter. Hence, nutritional management, especially the choice of dietary nutrient levels, is important to promote the growth of goats [3].…”

Section: Introductionmentioning

confidence: 99%

“…Previous studies showed that the phenotypic traits of ruminants were affected by rumen microbiota, whose functions could be re ected by the ruminal metabolites [23][24][25]. However, most studies have only focused on the single change of dietary protein/energy and few reports studied the comprehensive effects of dietary energy and protein on the rumen bacterial composition and rumen metabolites [3,16,18,22,26,27].…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Dynamic alterations in rumen bacterial community and metabolome characteristics in response to feed nutrient levels of cashmere goats

Wang

Tang

Xiao

et al. 2020

Preprint

View full text Add to dashboard Cite

Background: Dietary energy and protein play important roles in rumen fermentation. However, the comprehensive impacts of dietary energy and protein on rumen bacterial composition and ruminal metabolites were largely unknown. Therefore, the objective of the current study was to investigate the changes in rumen bacterial community and metabolites in response to the diets with simultaneous changes of dietary energy and protein levels in Shaanbei white cashmere goats (SWCG). Methods: A total of 12 ruminal samples were collected from SWCG, which were divided into two groups, including high-energy and high-protein (Group H; crude protein, CP: 9.37% in dry matter; metabolic energy, ME: 9.24 MJ/kg) or control (Group C; CP: 8.73%; ME:8.60 MJ/kg) groups. The experiment lasted for 65 days, including 10 days for adaptation. 16S rRNA gene sequencing and quantitative real-time polymerase chain reaction (qRT-PCR) were performed to identify the rumen bacterial community. Metabolomics analysis was done to investigate the rumen metabolites and the related metabolic pathways in Groups C and H. Results: The study observed 539 genera belonging to 30 phyla, which were distributed throughout the rumen samples. The high-energy and high-protein diets increased the relative abundance of phylum Bacteroidetes and genera Prevotella_1 and Succiniclasticum, while decreased the number of phylum Proteobacteria (p<0.05). Among the 24 differential metabolites (VIP>1.0, p<0.05) detected in this study, the dominant differential metabolites were amino acids, peptides and analogs. Tyrosine metabolism played an important role among the 9 main metabolic pathways. Correlation analysis revealed that Prevotella_1 showed strong positive correlation with 5-methoxyindole-3-acetic acid (r=0.601, p<0.05) and catechol (r=0.608, p<0.05). Succiniclasticum was positively correlated with 2-ketoadipate (r=0.741, p<0.01). Conclusions: Our findings revealed that the diets with high energy and protein in Group H significantly altered the composition of ruminal bacteria and metabolites, which could help to improve the dietary energy and protein use efficiency in goats.

show abstract

Section: Comparison Of the Composition And Differences Of Ruminal Bacsupporting

confidence: 92%

Section: Comparison Of the Composition And Differences Of Ruminal Bacmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Dynamic alterations in rumen bacterial community and metabolome characteristics in response to feed nutrient levels of cashmere goats

Wang

Tang

Xiao

et al. 2020

Preprint

View full text Add to dashboard Cite

show abstract

“…Then, the volatile fatty acids (VFAs) were measured by a GC (GC-2014 Shimadzu Corporation, Kyoto, Japan). Analysis was undertaken following our previous method [17]. Briefly, controlling the column nitrogen flow rate at 46.3 cm/s, the sampling amount was 0.4 µL, and the inlet temperature was maintained at 220 • C. The initial column temperature was set at 110 • C and held for 30 s; then, the temperature was increased at 10 • C/min to 120 • C and maintained for 4 min; finally, the temperature was increased at 10 • C/min to 150 • C. The detector temperature remained at 250 • C. The concentration of rumen fluid NH 3 -N was measured according to alkaline phenol hypochlorite method [18].…”

Section: Rumen Fermentation Parametersmentioning

confidence: 99%

Effects of Dietary Energy on Growth Performance, Rumen Fermentation and Bacterial Community, and Meat Quality of Holstein-Friesians Bulls Slaughtered at Different Ages

Wang

et al. 2019

Animals

Self Cite

View full text Add to dashboard Cite

The objective of this study was to evaluate the effects of dietary energy levels on growth performance, rumen fermentation and bacterial community, and meat quality of Holstein-Friesians bulls slaughtered at different ages. Thirty-six Holstein-Friesians bulls (17 months of age) were divided into a 3 × 3 factorial experiment with three energy levels (LE, ME and HE; metabolizable energy is 10.12, 10.90 and 11.68 MJ/kg, respectively) of diets, and three slaughter ages (20, 23 and 26 months). Results indicated that bulls fed with ME and HE diets had higher dry matter intake, average daily gain, and dressing percentage at 23 or 26 months of age. The ME and HE diets also reduced bacterial diversity, altered relative abundances of bacteria and produced lower concentrations of acetate, but higher butyrate and valerate concentrations in rumen fluid. Increasing in dietary energy and slaughter age increased the intramuscular fat (IMF) and water holding capacity. In summary, Holstein-Friesians bulls fed with ME and HE diets, slaughtered at 23 and 26 months of age could be a good choice to produce beef with high IMF. Slaughter age may have less influence than dietary energy in altering fermentation by increasing amylolytic bacteria and decreasing cellulolytic bacteria, and thus, further affecting meat quality.

show abstract

Rumen Buffers to Harness Nutrition, Health and Productivity of Ruminants

Kumar,

Tariq,

Mohanta

et al. 2024

Feed Additives and Supplements for Ruminants

View full text Add to dashboard Cite

Rumen fermentation, intramuscular fat fatty acid profiles and related rumen bacterial populations of Holstein bulls fed diets with different energy levels

Cited by 73 publications

References 55 publications

Dynamic alterations in rumen bacterial community and metabolome characteristics in response to feed nutrient levels of cashmere goats

Dynamic alterations in rumen bacterial community and metabolome characteristics in response to feed nutrient levels of cashmere goats

Effects of Dietary Energy on Growth Performance, Rumen Fermentation and Bacterial Community, and Meat Quality of Holstein-Friesians Bulls Slaughtered at Different Ages

Rumen Buffers to Harness Nutrition, Health and Productivity of Ruminants

Contact Info

Product

Resources

About