Rumen bacteria for evaluation of enzymatically changed animal feeds and genetic varieties of fodder plants

Meer, J.M. van der; Wever, G.; Bediye, Seyoum

doi:10.1016/s0003-2670(00)81351-3

Cited by 7 publications

(5 citation statements)

References 5 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In vivo digestibility of animal feeds can be estimated by measuring in-vitro gas production of feed samples incubated in ruminal fluid buffered with HC03-IC02 (Menke et al, 1979). This method can also be used to measure the degradation rates of feedstuffs by following gas production over time (Van der Meer et al, 1988; Krishnamoorty et al, 1991;Merry et al, 1991).…”

Section: Introductionmentioning

confidence: 99%

Modeling gas production kinetics of grass silages incubated with buffered ruminal fluid1

Beuvink

Kogut

1993

Journal of Animal Science

View full text Add to dashboard Cite

Time course measurements of in vitro gas production of feedstuffs incubated with buffered ruminal fluid give information about the rate at which the feed is fermented. To compare gas production kinetics from different feeds, a mathematical model was needed to describe the data. We investigated several existing models (exponential, logistic, Gompertz, Richards, Schnute), fitting them to 50 gas production curves obtained with grass silages. None of them gave a satisfactory description of the data. A new model was developed (modified Gompertz model; mGom) that basically divided gas production into two fractions, one arising from rapidly fermentable feed components and the other from slowly fermentable feed components. Residual mean squares (RMS) for the mGom model were lower (P < .05; n = 50) than the RMS for the other models. A good statistical comparison of kinetic gas production data is made possible with the mGom model.

show abstract

Section: Introductionmentioning

confidence: 99%

Modeling gas production kinetics of grass silages incubated with buffered ruminal fluid1

Beuvink

Kogut

1993

Journal of Animal Science

View full text Add to dashboard Cite

show abstract

“…This method was also applied to measure the degradation rates of feedstuffs by following GP over time (Van der Meer et al . 1988; Krishnamoorthy et al . 1991; Merry et al .…”

Section: Discussionmentioning

confidence: 99%

“…In vivo digestibility of animal feeds was estimated by measuring in vitro GP of feed samples incubated in ruminal fluid buffered with HCO 3 − /CO 2 (Menke et al 1979). This method was also applied to measure the degradation rates of feedstuffs by following GP over time (Van der Meer et al 1988;Krishnamoorthy et al 1991;Merry et al 1991). In the present study, differences between HF and LF diets were observed in GP kinetics, suggesting that the extent and rate of the feed degradation depended mainly on the nature of the incubated substrate.…”

Section: Influence Of Diet and Melamine On Gas Production Kinetics Anmentioning

confidence: 99%

Effect of melamine onin vitrorumen microbial growth, methane production and fermentation of Chinese wild rye hay and maize meal in binary mixtures

et al. 2013

View full text Add to dashboard Cite

The effects of melamine on gas production (GP) kinetics, methane (CH 4 ) production and fermentation of diets differing in forage content (low-forage (LF) diet: 200 g/kg and high-forage (HF) diet: 800 g/kg) by rumen micro-organisms in vitro were studied using batch cultures. Rumen contents were collected from three Simmental × Luxi crossbred beef cattle. Melamine was added to the incubation bottles to achieve final concentration of 0 (control), 2, 6, 18, 54, 162 and 484 mg/kg of each diet. Cumulative GP was continuously measured in an automated gas recording instrument during 72 h of incubation, while fermentation gas endproducts were collected to determine molar proportions of carbon dioxide (CO 2 ), CH 4 and hydrogen gas (H 2 ) in manually operated batch cultures. Differences in GP kinetics and fermentation gases were observed in response to the nature of the diets incubated. Although melamine addition did not affect GP kinetics and fermentation gas pattern compared to the control, the increase of melamine addition stimulated the yield of CH 4 by decreasing CO 2 , especially during the fermentation of the HF diet. The concentrations of ammonia nitrogen (N), amino acid N and microbial N in culture fluids were greater in the fermentation of LF-than HF diets, and these concentrations were increased by the increase of melamine addition after 72-h fermentation. The concentrations of total volatile fatty acids (VFA) were greater in HF than LF diets. The addition of melamine decreased total VFA concentrations and this response was greater in HF than LF diet fermentations. Melamine addition did not affect molar proportions of acetate, butyrate, propionate and valerate compared with the control; however, branched-chain VFA production, which was lower in the HF than the LF diet, was increased by the melamine addition, especially in the HF diet fermentation. The ratio of non-glucogenic to glucogenic acids was lower in the HF than the LF diet, but it was not affected by melamine addition. In brief, the greater reduction in the rate and extent of rumen fermentation found for the HF diet in comparison with the LF diet suggested that rumen fermentation rate and extent in vitro depended mainly on the nature of the incubated substrate, and that they could be further inhibited by the increase of melamine addition.

show abstract

“…The non-automated method of measuring gas production in time has been used in evaluation of genetic varieties of fodder plants (van der Meer et al, 1988), estimation of starch and protein availability in processed sorghum grain (Xiong et al, 1990) and determining the rate of fermentation of energy supplements (Krishnamoorty et al, 1991). The automated method presented in this paper allows easier measurement of rumen fermentation kinetics in vitro.…”

Section: Discussionmentioning

confidence: 99%

“…Gas production is directly related to rumen fermentation, relatively easy to mea sure and therefore appears to be a suitable parameter for rumen fermentation rate studies. The methods described so far (Van der Meer et al, 1988;Merry et al, 1991) require frequent periodical readings for over 48 h, making them less fit as routine laboratory methods. We describe here a system for automated registration of gas production in time.…”

Section: Introductionmentioning

confidence: 99%

An automated method for measuring time-course of gas production of feedstuffs incubated with buffered rumen fluid

Beuvink¹,

Spoelstra²,

Hogendorp³

1992

NJAS

View full text Add to dashboard Cite

A system for automated registration of gas production in time upon incubation of feedstuffs with rumen fluid is presented. The system is based on weighing the amount of fluid replaced by fermentation gas, followed by calculation to gas volume and registration by a data logger. Incubations with glucose, rice starch and crystalline cellulose showed glucose to be fermentated at the fastest rate and cellulose at the slowest. The major source of variation was rumen fluid from different days. An example of a gas production curve is given for the incubation of grass and its cell wall fraction (obtained after treatment with Neutral Detergent reagent).

show abstract

Rumen bacteria for evaluation of enzymatically changed animal feeds and genetic varieties of fodder plants

Cited by 7 publications

References 5 publications

Modeling gas production kinetics of grass silages incubated with buffered ruminal fluid1

Modeling gas production kinetics of grass silages incubated with buffered ruminal fluid1

Effect of melamine onin vitrorumen microbial growth, methane production and fermentation of Chinese wild rye hay and maize meal in binary mixtures

An automated method for measuring time-course of gas production of feedstuffs incubated with buffered rumen fluid

Contact Info

Product

Resources

About