Rugamonas aquatica sp. nov. and Rugamonas rivuli sp. nov., isolated from a subtropical stream in PR China

Abstract

<jats:p>Two Gram-stain-negative, catalase- and oxidase-positive, rod-shaped, motile strains (FT29W<jats:sup>T</jats:sup> and FT103W<jats:sup>T</jats:sup>) were isolated from a subtropical stream in PR China. Comparisons based on 16S rRNA gene sequences showed that strains FT29W<jats:sup>T</jats:sup> and FT103W<jats:sup>T</jats:sup> showed 98.7 and 98.6 % 16S rRNA gene sequence similarities to <jats:italic> <jats:named-content content-type="species"> <jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="uri" xlink:href="http://doi.org/10.1601/nm.2742" xlink:type="simple">Rugamonas rubra</jats:ext-link> </jats:named-content> </jats:italic> CCM 3730<jats:sup>T</jats:sup> as their closest neighbour, respectively. The calculated pairwise OrthoANIu values between strain <jats:italic> <jats:named-content content-type="species"> <jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="uri" xlink:href="http://doi.org/10.1601/nm.2742" xlink:type="simple">R. rubra</jats:ext-link> </jats:named-content> </jats:italic> CCM 3730<jats:sup>T</jats:sup> and strains FT29W<jats:sup>T</jats:sup> and FT103W<jats:sup>T</jats:sup> were all 81.4 %. The respiratory quinone of strains FT29W<jats:sup>T</jats:sup> and FT103W<jats:sup>T</jats:sup> was determined to be Q-8. The major fatty acids were C<jats:sub>16 : 1</jats:sub> ω7<jats:italic>c</jats:italic>, C<jats:sub>16 : 0</jats:sub> and C<jats:sub>12 : 0</jats:sub>. The polar lipids of strain FT103W<jats:sup>T</jats:sup> included phosphatidylethanolamine, phosphatidylglycerol and one unidentified phospholipid, but strain FT29W<jats:sup>T</jats:sup> did not contain phosphatidylglycerol. The genome sizes of strains FT29W<jats:sup>T</jats:sup> and FT103W<jats:sup>T</jats:sup> were 7.23 and 6.84 Mbp with G+C contents of 63.8 and 63.9 %, respectively. Although the pairwise OrthoANIu value between strains FT29W<jats:sup>T</jats:sup> and FT103W<jats:sup>T</jats:sup> was 95.2 % which located in the transition region of species demarcation, the dissimilarities in the aspects of phenotypic, biochemical and genotypic characteristics supported these two strains should belong to the different species within genus <jats:italic> <jats:named-content content-type="genus"> <jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="uri" xlink:href="http://doi.org/10.1601/nm.2741" xlink:type="simple">Rugamonas</jats:ext-link> </jats:named-content> </jats:italic>, for which the names <jats:italic>Rugamonas aquatica</jats:italic> sp. nov. (type strain FT29W<jats:sup>T</jats:sup>=GDMCC 1.1643<jats:sup>T</jats:sup>=KACC 21316<jats:sup>T</jats:sup>) and <jats:italic>Rugamonas rivuli</jats:italic> sp. nov. (type strain FT103W<jats:sup>T</jats:sup>=GDMCC 1.1685<jats:sup>T</jats:sup>=KACC 21477<jats:sup>T</jats:sup>) are proposed.</jats:p>

Classification of a Violacein-Producing Psychrophilic Group of Isolates Associated with Freshwater in Antarctica and Description of Rugamonas violacea sp. nov.

“…nov. and R. rivuli KACC 21477 T based on presence of C 17:0 cyclo. Two other validly named Rugamonas species, R. rubra and R. aquatica , show only minor qualitative differences presented in Table 6 and described by Lu et al ( 3 ). The absence of C 12:0 2OH, C 12:0 3OH, iso-C 16:0 , and C 17:0 cyclo among the analyzed strains clearly distinguished those from violacein-producing species Chromobacterium violaceum , Janthinobacterium lividum , or Iodobacter fluviatile ( Table 6 ).…”

“…There are only three species with validly published names in the genus Rugamonas ( http://lpsn.dsmz.de/genus/rugamonas ) ( 2 ), and these species are associated with aquatic environments. Until recently, the species R. rubra was the sole taxon of the genus; however, two new species, Rugamonas aquatica and Rugamonas rivuli , both from a subtropical stream, were described recently ( 3 ). Both these novel species have light brown colonies.…”

Duganella vulcania sp. nov., Rugamonas fusca sp. nov., Rugamonas brunnea sp. nov. and Rugamonas apoptosis sp. nov., isolated from subtropical streams, and phylogenomic analyses of the genera Janthinobacterium, Duganella, Rugamonas, Pseudoduganella and Massilia

“…aquatica ’ FT29W and ‘ D. aquatica ’ FT103W (both strains isolated from subtropical streams) were also used as reference strains for comparative studies with the isolated strains, but the experimental data cited is from our previous study [20].…”

“…The genus Rugamonas proposed by Austin and Moss in 1986 was mainly based on the numerical taxonomy methods [19]. Since then, only Rugamonas aquatica and Rugamonas rivuli isolated from subtropical streams in PR China [20] and Rugamonas violacea isolated from freshwater in Antarctica [21] have been reported. However, R.…”