“…The transition metal ions are extensively used for redox chemistry in a variety of chemical and biological transformations, but their specific stable oxidation state has been also used as a potential spectroscopic probe due to its inherent spectroscopic properties. Metal ions possessing observable spectroscopic features are used as probes in specific techniques including UV-vis, NMR, electron paramagnetic resonance (EPR), Mössbauer, fluorescence quencher and extended X-ray absorption fine structure in many metalloproteins ( Maiti et al., 2017a ; Hagen 2006 ; Holm et al., 1996 ). For example, Cu II is a paramagnetic species whereas Cu I is a diamagnetic species.…”
Section: Applications Of M-atcun Derivativesmentioning
confidence: 99%
“…It is only possible when two molecules interact with each other in head-to-tail fashion ( Figure 3 ). This Cu II -ATCUN-ORP derivative provides a model of intermolecular protein-protein interaction ( Maiti et al., 2017a , 2017b ). Figure 3 Application of Cu-ATCUNs as Paramagnetic NMR Reporters (A–C) (A) The paramagnetic NMR probe, Cu II -ATCUN is attached at N-terminus of ORP.…”
Section: Applications Of M-atcun Derivativesmentioning
confidence: 99%
“…Nature utilizes an efficient enzyme, Hydrogenases, that cleave heterolytically the hydrogen molecule in a reversible mode ( Maiti et al., 2017a ; Lubitz et al., 2014 ). H 2 production (an alternative source of fossil fuel) from the reduction of aqueous proton is one of the potential sustainable energy routes.…”
Section: Applications Of M-atcun Derivativesmentioning
Summary
The designed “ATCUN” motif (amino-terminal copper and nickel binding site) is a replica of naturally occurring ATCUN site found in many proteins/peptides, and an attractive platform for multiple applications, which include nucleases, proteases, spectroscopic probes, imaging, and small molecule activation. ATCUN motifs are engineered at periphery by conjugation to recombinant proteins, peptides, fluorophores, or recognition domains through chemically or genetically, fulfilling the needs of various biological relevance and a wide range of practical usages. This chemistry has witnessed significant growth over the last few decades and several interesting ATCUN derivatives have been described. The redox role of the ATCUN moieties is also an important aspect to be considered. The redox potential of designed M-ATCUN derivatives is modulated by judicious choice of amino acid (including stereochemistry, charge, and position) that ultimately leads to the catalytic efficiency. In this context, a wide range of M-ATCUN derivatives have been designed purposefully for various redox- and non-redox-based applications, including spectroscopic probes, target-based catalytic metallodrugs, inhibition of amyloid-β toxicity, and telomere shortening, enzyme inactivation, biomolecules stitching or modification, next-generation antibiotic, and small molecule activation.
“…The transition metal ions are extensively used for redox chemistry in a variety of chemical and biological transformations, but their specific stable oxidation state has been also used as a potential spectroscopic probe due to its inherent spectroscopic properties. Metal ions possessing observable spectroscopic features are used as probes in specific techniques including UV-vis, NMR, electron paramagnetic resonance (EPR), Mössbauer, fluorescence quencher and extended X-ray absorption fine structure in many metalloproteins ( Maiti et al., 2017a ; Hagen 2006 ; Holm et al., 1996 ). For example, Cu II is a paramagnetic species whereas Cu I is a diamagnetic species.…”
Section: Applications Of M-atcun Derivativesmentioning
confidence: 99%
“…It is only possible when two molecules interact with each other in head-to-tail fashion ( Figure 3 ). This Cu II -ATCUN-ORP derivative provides a model of intermolecular protein-protein interaction ( Maiti et al., 2017a , 2017b ). Figure 3 Application of Cu-ATCUNs as Paramagnetic NMR Reporters (A–C) (A) The paramagnetic NMR probe, Cu II -ATCUN is attached at N-terminus of ORP.…”
Section: Applications Of M-atcun Derivativesmentioning
confidence: 99%
“…Nature utilizes an efficient enzyme, Hydrogenases, that cleave heterolytically the hydrogen molecule in a reversible mode ( Maiti et al., 2017a ; Lubitz et al., 2014 ). H 2 production (an alternative source of fossil fuel) from the reduction of aqueous proton is one of the potential sustainable energy routes.…”
Section: Applications Of M-atcun Derivativesmentioning
Summary
The designed “ATCUN” motif (amino-terminal copper and nickel binding site) is a replica of naturally occurring ATCUN site found in many proteins/peptides, and an attractive platform for multiple applications, which include nucleases, proteases, spectroscopic probes, imaging, and small molecule activation. ATCUN motifs are engineered at periphery by conjugation to recombinant proteins, peptides, fluorophores, or recognition domains through chemically or genetically, fulfilling the needs of various biological relevance and a wide range of practical usages. This chemistry has witnessed significant growth over the last few decades and several interesting ATCUN derivatives have been described. The redox role of the ATCUN moieties is also an important aspect to be considered. The redox potential of designed M-ATCUN derivatives is modulated by judicious choice of amino acid (including stereochemistry, charge, and position) that ultimately leads to the catalytic efficiency. In this context, a wide range of M-ATCUN derivatives have been designed purposefully for various redox- and non-redox-based applications, including spectroscopic probes, target-based catalytic metallodrugs, inhibition of amyloid-β toxicity, and telomere shortening, enzyme inactivation, biomolecules stitching or modification, next-generation antibiotic, and small molecule activation.
“…The effect of metals added to the values of energy gap was shown in Table 3 The energy gap shift could be assigned to the polarons formation in the doped films (8). The polymer-metal composites may be recognized by interaction of metal with the polar group that exists in polymeric chain.…”
Section: Optical Propertiesmentioning
confidence: 99%
“…The physiological importance of oxygen donor in the organic compounds increasing the active role acted by coordinating certain metal ions to utilize in studying the structural aspects and synthesizing metal complexes with some sulphur, nitrogen and oxygen donor ligands (8)(9)(10). The aim of this work is to study transition metals influence on the optical, electrical properties and surface morphology of the novel materials.…”
In this work, synthesis of conducting polymeric films namely, PVC thin films was carried out containing Schiff base (L) with Cu2+, Cr3+, Ni2+, Co2+, in addition to inspecting the possibilities of measuring energy gap values of PVC-L-M with variety metal ions. These new polymeric films (PVC-L-M) were characterized by FTIR spectrophotometry, energy gap and surface morphology. The optical data recorded that the band gap values are influenced by the type of metals. All modified films have a red shift in optical properties in the ultraviolet region. The PVC-L-Co(II) was the lowest value of the optical band gap, 3.1 eV.
While Fenton chemistry has long been known to play a role in inducing cellular stress, there has been recent renewed interest in understanding its roles in health and disease. Here we describe a fluorogenic rhodamine‐thiophenol probe, RTFt1, which applies both chelation and recognition strategies to sense the Fenton reactants, Fe(II) and H2O2. RTFt1 undergoes a 200‐fold increase in red fluorescence emission in the presence of both Fe(II) and H2O2. After confirming the suitability of this new probe in cellular models, we demonstrated its utility in sensing the Fenton chemistry that accompanies ferroptosis and cisplatin‐induced cytotoxicity. We found that levels of the Fenton reactants increased when cells were treated with cisplatin or co‐treated with cisplatin and tubastatin A. This points to a role for Fenton chemistry in the cytotoxic effects of these treatments. We expect that RTFt1 will be able to provide a deeper understanding of Fenton reactants more broadly in biological systems.
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