Rubidium-87 magnetic resonance spectroscopy and imaging for analysis of mammalian K+ transport

Kupriyanov, V.V.; Gruwel, Marco L.H.

doi:10.1002/nbm.892

Cited by 21 publications

(12 citation statements)

References 66 publications

(173 reference statements)

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“…This includes studies on isolated perfused rat and pig hearts and also on kidney, skeletal muscle, salivary gland and red blood cells. The method has been used for measuring the kinetics of unidirectional rubidium uptake and efflux and steady-state rubidium levels [285]. For example, 3D 87 Rb-MRI analysis of isolated pig hearts allowed the effects of regional ischemia on rubidium uptake to be investigated.…”

Section: Krypton ( 83mentioning

confidence: 99%

NMR-Active Nuclei for Biological and Biomedical Applications

Patching¹

2016

J Diagn Imaging Ther

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Nuclear magnetic resonance (NMR) spectroscopy is a principal well-established technique for analysis of chemical, biological, food and environmental samples. This article provides an overview of the properties and applications of NMR-active nuclei (39 nuclei of 33 different elements) used in NMR measurements (solution-and solid-state NMR, magnetic resonance spectroscopy, magnetic resonance imaging) with biological and biomedical systems and samples. The samples include biofluids, cells, tissues, organs or whole body from different organisms (humans, animals, bacteria, fungi, plants) for detecting and quantifying metabolites or environmental samples (water, soils, sediments). Isolated biomolecules (peptides, proteins, nucleic acids) can be analysed for elucidation of atomic-resolution structure, conformation and dynamics and for characterisation of ligand and drug binding, and of protein-ligand, protein-protein and protein-nucleic acid interactions. NMR can be used for drug screening and pharmacokinetics and to provide information in the design and discovery of new drugs. NMR can also measure translocation of ions and small molecules across lipid bilayers and membranes, characterise structure, phase behaviour and dynamics of membranes and elucidate atomic-resolution structure, orientation and dynamics of membrane-embedded peptides and proteins.Keywords: biological and biomedical applications; drug screening; dynamics; magnetic resonance imaging; membrane proteins; metabolomics; MRI; NMR-active nuclei; nuclear magnetic resonance; protein structure INTRODUCTION1 UCLEAR magnetic resonance (NMR) spectroscopy is one of the principal techniques used for analysis of biological and biomedical systems and samples. This can include the identification, quantification and monitoring of ions, small molecules and biomolecules in studies of metabolism and biological function in human and animal cells and tissues, bacterial cells and spores, fungi and plants. Similar types of measurements can be performed on environmental samples such as water, soils and sediment. NMR is able to elucidate atomic-resolution structure, conformation, molecular mechanism, dynamics and exchange processes (on timescales of picoseconds to seconds) in biomolecules, especially peptides, proteins and nucleic acids. NMR can be used for the observation, quantification and characterisation of ligand and drug binding to biomolecules, and for characterisation of ligandprotein, protein-protein and protein-nucleic acid interactions. NMR can be used for drug screening and it can acquire structural, binding and kinetic information for the design and discovery of new drugs. It can then monitor the absorption, distribution, metabolism and excretion (ADME) of administered drugs in pharmacokinetics studies. OPEN ACCESS PEER-REVIEWED*NMR can be used for the observation, quantification and kinetic characterisation of ion and smallmolecule translocation across lipid bilayers and biological membranes, including those of cells, tissues and vesicles. Solid-state NM...

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Section: Krypton ( 83mentioning

confidence: 99%

NMR-Active Nuclei for Biological and Biomedical Applications

Patching¹

2016

J Diagn Imaging Ther

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“…Their concentrations in the solution were both 0.375 mmol/ml to achieve doses of 0.75 mmol/kg. Lithium and rubidium were utilized as surrogate electrolyte markers for sodium and potassium, respectively [14][15][16]. After that, 100 ml of blood was taken from the cervical vein at 5, 15, 40, 60, 120 and 180 min into a heparinized tube, and it was then centrifuged at 8000 Â g for 10 min at 41C to collect a plasma specimen.…”

Section: Evaluation Of Plasma Concentration Profiles Of Marker Electrmentioning

confidence: 99%

Altered electrolyte handling of the choroid plexus in rats with glycerol‐induced acute renal failure

Ishikawa

Kono

Sakae

et al. 2010

Biopharm & Drug Disp

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The altered electrolyte handling of the choroid plexus was investigated in rats with acute renal failure (ARF) using lithium and rubidium as surrogate markers for sodium and potassium, respectively. Firstly, the transport of these two markers from the plasma to cerebrospinal fluid (CSF) was evaluated after they were concurrently injected into the femoral vein. As a result, their disposition from the plasma to CSF was shown to decrease in ARF rats, but the relationship profile between those two markers was not different from that observed in normal rats, indicating that the decreased disposition of lithium and rubidium occurs without affecting the stoichiometric balance. To clarify the mechanisms accounting for the decreased disposition, an inhibition study was then performed. When bumetanide, an inhibitor of the Na(+) /K(+) /2Cl(-) co-transporter, was directly introduced into the cerebroventricle prior to lithium and rubidium being intravenously administered, a marked increase in the markers' disposition was observed. However, such an increased disposition did not occur when bumetanide was injected into the femoral vein. Other inhibitors, such as amiloride for the Na(+) /H(+) exchanger and ouabain for Na(+) /K(+) -ATPase, did not show any effects on marker disposition regardless of the inhibitor being administered into either the cerebroventricle or femoral vein. These findings suggest that the decreased marker disposition in ARF rats is due to an increased efflux process of the choroid plexus mediated by the Na(+) /K(+) /2Cl(-) co-transporter. That is, electrolyte efflux from the CSF to plasma increases, and thereby the electrolyte influx from the plasma to CSF is counteracted.

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“…Marco Gruwel and myself put together the data available on 87 Rb MRS/I applications. 16 The article contains some necessary background information on K þ and Rb þ transport in cardiac muscle, NMR properties of Rb isotopes, and results of early spectroscopic studies. Studies of K þ transport in isolated hearts using pharmacological analysis of Rb þ uptake and efflux under normal and pathological conditions are summarized.…”

Section: Almentioning

confidence: 99%