Rubella virus and rheumatoid arthritis.

Hart, H; Marmion, B. P.

doi:10.1136/ard.36.1.3

Cited by 28 publications

(17 citation statements)

References 39 publications

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“…The solid-phase radioimmunoassay for antigen detection (Forghani et al, 1974;Hart and Marmion, 1977) was adopted for detection of antibody by extending the times of incubation to 16 hours at 40C for the first antibody reaction and copyright. Complement-mediated cell cytotoxicity.…”

Section: Methodsmentioning

confidence: 99%

Viruses and lymphocytes in rheumatoid arthritis. II. Examination of lymphocytes and sera from patients with rheumatoid arthritis for evidence of retrovirus infection.

Hart¹,

McCormick²,

Marmion³

1979

Annals of the Rheumatic Diseases

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summARY The possible involvement of retroviruses in the aetiology of rheumatoid arthritis (RA) was investigated. Retrovirus antigens were not expressed on rheumatoid synovial and peripheral blood lymphocytes as judged by membrane immunofluorescence, radioimmunoassay, and complement-mediated cytotoxicity. The specific antiretroviral (anti-RD-144 and anti-SSAV) sera used in this study were produced in rabbits immunised with viral antigens grown in a homologous system (rabbit cells and medium supplemented with normal rabbit serum), avoiding non-specific immunofluorescence previously detected with donated antiretroviral sera. Immune complexes lodged in the rheumatoid synovial membranes did not contain, and other cells within the membranes did not express, retroviral antigens. Antibodies cross-reacting with primate retrovirus antigens were sought in sera from patients with 'autoimmune' diseases by means of solid phase radioimmunoassay. There were no retrovirus antibodies in the 3 groups of patients studied, that is, those with rheumatoid arthritis, systemic lupus erythematosus, and with non-RA conditions. Absorption of rheumatoid factor did not alter this conclusion. These results give little support to the hypothesis that activation of endogenous human retroviruses or an infection with horizontally transmitted retroviruses is associated with the rheumatoid process.

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Section: Methodsmentioning

confidence: 99%

Viruses and lymphocytes in rheumatoid arthritis. II. Examination of lymphocytes and sera from patients with rheumatoid arthritis for evidence of retrovirus infection.

Hart¹,

McCormick²,

Marmion³

1979

Annals of the Rheumatic Diseases

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“…Several investigators studied specific virus antibodies in synovial tissue eluates, synovial fluids, and cryoprecipitates from them. As with serum levels in RA, there were no striking abnormalities (25)(26)(27). I n sum, there is no direct evidence for microbial involvement in RA (22).…”

Section: Discussionmentioning

confidence: 99%

“…By means of immunofluorescence, a possibly rubellarelated antigen was found in some juvenile RA, but not adult RA, synovial fluids; the specificity of the staining was not shown, however (23). A brief report of the -specific cytotoxicity of rubella antisera for RA synovial cultures (24) was not confirmed by a detailed study using immunofluorescent and radioimmunologic methods to detect rubella-related antigens in RA cultures (25). Several investigators studied specific virus antibodies in synovial tissue eluates, synovial fluids, and cryoprecipitates from them.…”

Section: Discussionmentioning

confidence: 99%

Immunopathologic studies of rheumatoid arthritis.

Paget¹,

Anderson²,

Phillips³

1978

Arthritis & Rheumatism

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A sensitive complement‐dependent chromium release cytotoxicity assay was used to determine whether sera from rheumatoid arthritis (RA) patients contain antibody specific for an antigen on rheumatoid synovial cell cultures. Two hundred eight RA sera–RA synovial culture combinations were studied employing 21 sera and 16 synovial membranes; control combinations were derived from 5 normal sera and 10 degenerative joint disease synovial membranes. Anticomplementary activity of some rheumatoid sera was overcome using an increased complement concentration. The percent cytotoxicity of RA serum–RA culture combinations, both homologous and autologous, was not significantly greater than that of RA serum–control culture combinations. No correlation between duration of disease or duration of cell culture and percent cytotoxicity was found. Thus a unique antigen on cultured rheumatoid synovial cells was not recognized by rheumatoid serum antibody by use of this cytotoxicity assay.

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“…This was carried out as described previously (Hart and Marmion, 1977) with coverslips fixed in acetone after the 3 hour incubation. The specific antisera used were all prepared in rabbits and have been described before together with appropriate control anticell sera.…”

Section: Imm Unofl Uorescencementioning

confidence: 99%

Search for viruses in rheumatoid macrophage-rich synovial cell populations.

Hart¹,

Norval²

1980

Annals of the Rheumatic Diseases

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SUMMARY The adherent cells remaining after short-term culture of synovial fluid and synovial membrane cells from rheumatoid and non-rheumatoid patients were examined for the presence of a productive virus infection and for various viral antigens. Labelling was carried out with 3H-thymidine and 3H-uridine followed by sucrose density gradient centrifugation of the culture supernatant. Only in 1 case was there incorporation of 3H-uridine into material of density 1 .21 g/cm3. Viral antigens were tested for by indirect immunofluorescence with antisera to rubella virus, the retroviruses RD-1 14 and simian sarcoma associated virus, early adenovirus type 2 antigens, late adenovirus type 2 antigens, SV-40 T antigen, and in 1 case measles virus. No cell showed immunofluorescence with any antiserum except the early adenovirus type 2 antiserum, which stained the cytoplasm of about half the synovial cell cultures, some from rheumatoid and some from non-rheumatoid patients.

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Rubella virus and rheumatoid arthritis.

Cited by 28 publications

References 39 publications

Viruses and lymphocytes in rheumatoid arthritis. II. Examination of lymphocytes and sera from patients with rheumatoid arthritis for evidence of retrovirus infection.

Viruses and lymphocytes in rheumatoid arthritis. II. Examination of lymphocytes and sera from patients with rheumatoid arthritis for evidence of retrovirus infection.

Immunopathologic studies of rheumatoid arthritis.

Search for viruses in rheumatoid macrophage-rich synovial cell populations.

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