[Ru(bpy)<sub>2</sub>dppz]<sup>2+</sup> Electrochemiluminescence Switch and Its Applications for DNA Interaction Study and Label-free ATP Aptasensor

Hu, Lianzhe; Bian, Zheng; Li, Haijuan; Han, Shuang; Yuan, Yali; Gao, Lianxun; Xu, Guobao

doi:10.1021/ac901925x

Cited by 140 publications

(98 citation statements)

References 53 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…It also displays negligible ECL in the oxalate solution, while intercalating into the nucleic acid structure its ECL increases about 1,000 times as shown in Fig. 4.2 [64] which is attributed to the intercalation that shielded the phenazine nitrogens from the solvent and resulted in a luminescent excited state (similar to the reason of its photoluminescence properties). This dramatic increase in ECL intensity upon intercalation makes [Ru(bpy) 2 (Fig.…”

Section: +mentioning

confidence: 92%

ECL Luminophores

Parveen¹,

Aslam²,

Hu³

et al. 2013

SpringerBriefs in Molecular Science

Self Cite

View full text Add to dashboard Cite

Finding new luminophores with higher ECL efficiencies and modifying a moiety of the emitter to use it for the labeling of biomolecules are the two driving forces that lead to the synthesis of a number of new ECL-emitting species in the past several years. Three categories of luminophores are discussed in this chapter, including (a) inorganic systems, which mainly contain organometallic complexes; (b) organic systems, which cover polycyclic aromatic hydrocarbons (PAHs); and (c) semiconductor nanoparticle systems.Keywords Luminophores Á Organic systems Á Inorganic systems Á Dendrimers Á Nanoparticle systems Á Nanoclusters Á Carbon nanoparticles Á Semiconductor nanocrystals Á Quantum dots Since the discovery of ECL process, there has been considerable advancement in ECL system. Different types of ECL systems have been introduced, such as organic, inorganic, and nanoparticle systems. Organic SystemsThe first exhaustive study on ECL involved organic systems [1][2][3]. In the early ages of ECL, PAHs, rubrene, and DPA have been studied extensively owing to their high fluorescence quantum yields and stable radical cations and anions in aprotic media. Some other organic systems, such as luminol [4], acridinium esters [5], polymers [6][7][8], and siloles [9], have also been widely explored. The reader is referred to the tremendous reviews [10][11][12] and ECL monograph [13] for more thorough study about organic systems. Acridinium esters, taking example of lucigenin (N,N'-dimethyl-9,9-bisacridinium) is reported ECL active [14] in the presence of hydrogen peroxide [15] and has been applied for the detection of riboflavin [16], human chorionic gonadotropin [17], and hemin [18]. The ECL reaction of methyl-9-(p-formylphenyl)acridinium carboxylate fluorosulfonate (MFPA) gives intense ECL signal and is employed as a label in bioassays [17]. Lucigenin ECL has also been extended to surfaces [19]. Self-assembled monolayers (SAMs) modified electrodes and solutions containing Triton X-100 surfactant molecules caused the enhancement in ECL of lucigenin.Fluorene-substituted PAHs have been investigated with enhanced ECL efficiency and stability [20]. Derivatives of DPA, pyrene, and anthracene were produced by using fluorene as a capping agent. These molecules have high PL quantum yields and can generate stable radical ions. On introducing the fluorene groups, steric hindrance is imparted, preventing interchromophore interactions and blocking the active position of PAH cores subject to electrochemical decomposition. Due to the electrochemical instability of its cationic radical, pyrene molecule displays poor ECL properties. Hence, a strategy for ECL enhancement and radical stability by peripheral multidonors on pyrene derivatives has been adopted in which the ECL efficiencies of pyrene derivatives increase in proportion to the number of peripheral N, N-dimethyl aniline donors [21].An ECL laser offers numerous advantages over traditional techniques, including tunability, lack of additional source and range of available wavelength...

show abstract

Section: +mentioning

confidence: 92%

ECL Luminophores

Parveen¹,

Aslam²,

Hu³

et al. 2013

SpringerBriefs in Molecular Science

Self Cite

View full text Add to dashboard Cite

show abstract

“…2 + as an ECL probe [9]. Bards group developed an ECL bioassays by labeling polystyrene microspheres preloaded with a large number of Ru(bpy) 3 2 + to target molecules, and then releasing the Ru(bpy) 3 2 + into an acetonitrile solution and measuring ECL of Ru(bpy) 3 2 + [10,11].…”

Section: Dppz]mentioning

confidence: 99%

Electrochemiluminescence DNA Sensor Based on Hairpin Structure DNA as recognition element and Ru(bpy)₃²⁺‐doped silica Nanoparticles as Signal‐Producing Compound

Sun¹,

Zou²,

Zhang³

2011

Electroanalysis

View full text Add to dashboard Cite

An electrochemiluminescence (ECL) DNA sensor was presented using a hairpin structure DNA as recognition element and Ru(bpy) 3 2 + -doped silica nanoparticles (Ru-DSNPs) as ECL signal-producing compound. The sensor was fabricated by immobilizing a hairpin structure DNA probe labeled with Ru-DSNPs onto a gold electrode and formimg the stem-loop structure. This sensor generated a strong ECL. After hybridization with the target DNA, the ECL signal significantly decreased. The logarithm of ECL intensity decreased linearly with the logarithm of target DNA concentration in the range from 2.0 10 À13 to 2.0 10 À9 mol/L with detection limit was 5.0 10 À14 mol/L (S/N = 3).

show abstract

“…Electrochemical properties of the complexes have been examined by cyclic voltammetry in CH 3 CN in presence of [n-Bu 4 N][ClO 4 ] as supporting electrolyte using a Pt-disc working electrode at the scan rate of 50 mV/s. Although several groups [6][7][8] have tried to investigate the electrochemical interaction of metalcytosine with different electrodes, our aim is to investigate the interaction of Cu(II) with cytosine in BR buffer medium maintaining various pH at GCE using cyclic voltammetry.…”

Section: Introductionmentioning

confidence: 99%

Interaction of Cu(II) with Cytosine in Britton-Robinson Buffer Solution - A Cyclic Voltammetric Study

Shaikh

Firdaws

Islam

et al. 2015

Dhaka Univ. J. Sci.

View full text Add to dashboard Cite

Electrochemical redox behavior of Cu(II) at different pH in Britton-Robinson (BR) buffer solution has been investigated using cyclic voltammetry at glassy carbon electrode (GCE). Cu(II) shows a cathodic peak and a hump-like peak, and an intense anodic peak at pH, 0.59, 1.59, 3.01 and 4.08. Although at lower pH (0.59 and 1.59) a cathodic peak, a hump and an anodic peak are observed, at higher pH (3.01 and 4.08) the hump-like cathodic peak disappeared. Cyclic voltammetry has also been used to observe the interaction of Cu(II) with cytosine in BR buffer solution at various pH. A reasonably strong interaction between Cu(II) and cytosine at different Cu(II)/cytosine molar ratio is observed at all the studied pH. However, maximum interaction occurs at 1:4 molar ratio at pH 4.08, this is perhaps the most suitable condition for Cu(II)-cytosine interaction.

show abstract

[Ru(bpy)₂dppz]²⁺ Electrochemiluminescence Switch and Its Applications for DNA Interaction Study and Label-free ATP Aptasensor

Cited by 140 publications

References 53 publications

ECL Luminophores

ECL Luminophores

Electrochemiluminescence DNA Sensor Based on Hairpin Structure DNA as recognition element and Ru(bpy)₃²⁺‐doped silica Nanoparticles as Signal‐Producing Compound

Interaction of Cu(II) with Cytosine in Britton-Robinson Buffer Solution - A Cyclic Voltammetric Study

Contact Info

Product

Resources

About

[Ru(bpy)2dppz]2+ Electrochemiluminescence Switch and Its Applications for DNA Interaction Study and Label-free ATP Aptasensor

Cited by 140 publications

References 53 publications

ECL Luminophores

ECL Luminophores

Electrochemiluminescence DNA Sensor Based on Hairpin Structure DNA as recognition element and Ru(bpy)32+‐doped silica Nanoparticles as Signal‐Producing Compound

Interaction of Cu(II) with Cytosine in Britton-Robinson Buffer Solution - A Cyclic Voltammetric Study

Contact Info

Product

Resources

About

[Ru(bpy)₂dppz]²⁺ Electrochemiluminescence Switch and Its Applications for DNA Interaction Study and Label-free ATP Aptasensor

Electrochemiluminescence DNA Sensor Based on Hairpin Structure DNA as recognition element and Ru(bpy)₃²⁺‐doped silica Nanoparticles as Signal‐Producing Compound