RT-qPCR Analysis of Host Defense-Related Genes in Nonhost Resistance: Wheat-Bgh Interaction

Rezaei, Ali; Mahdian, Safarali; Babaeizad, Valiollah; Hashemi-Petroudi, Seyyed Hamidreza; Alavi, Seyed Mohammad

doi:10.1134/s102279541903013x

Cited by 4 publications

(1 citation statement)

References 35 publications

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“…The expression and function of these genes are not isolated but form an internal defense network in plants, collectively participating in the ght against the invasion of external pathogens. Plants are capable of constructing a multi-tiered defense system to effectively resist various pathogens (Li et al, 2021b;Rezaei et al, 2019;Nie et al, 2017). In the defense of cotton against Verticillium wilt, the expression of disease resistance-related genes is induced, which helps to enhance the disease resistance of cotton and reduce the impact of the disease.…”

Section: Introductionmentioning

confidence: 99%

The Impact of Biocontrol Bacteria on Cotton Resistance and Their Effects on Signaling Pathways Related to Defense Against Verticillium Wilt Infection

Fan,

Cao,

Liu

et al. 2024

Preprint

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This study prepared a mixed fermentation broth using two strains of Bacillus and investigated its inhibitory effects on the cotton Verticillium wilt pathogen, as well as its impact on the signaling pathways related to defense against Verticillium wilt infection in cotton.Biocontrol bacteria can effectively defend against plant diseases by competitively inhibiting pathogens and inducing plant immunity. Through plate confrontation assays, antimicrobial tests using mixed microbial fermentation broth and its dilutions, and their impacts on cotton seed germination, this study explores the defensive potential of the mixed fermentation broth.During the study, it was discovered that The mixed microbial fermentation broth could produce lipopeptide substances. The cotton's immunity against Verticillium wilt, following treatment with this broth, was assessed using DAB and trypan blue histological staining methods. Furthermore, the study involved monitoring the induced expression of resistance-related genes (PR1, PR5, NPR1), as well as the effects on the activities of defense-related enzymes in cotton (SOD, CAT, PPO, POD).The results indicate that The combination of two biocontrol bacterial strains exhibited a certain inhibitory effect on the cotton Verticillium wilt pathogen. Root drenching with the mixed fermentation broth significantly enhanced the transient burst of reactive oxygen species in cotton's defense signaling pathways, inducing an immune response. This response increased the sensitivity of cotton's hypersensitive response (HR), induced the expression of disease resistance-related genes, and heightened the activity of enzymes involved in reactive oxygen species scavenging, thereby enhancing systemic acquired resistance (SAR) in cotton. This study reveals that the mixed fermentation broth improved cotton's resistance to Verticillium wilt, significantly affecting the defense signaling pathways in response to the pathogen, with varying effects on induced resistance in different resistance genotypes of cotton.

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Section: Introductionmentioning

confidence: 99%

The Impact of Biocontrol Bacteria on Cotton Resistance and Their Effects on Signaling Pathways Related to Defense Against Verticillium Wilt Infection

Fan,

Cao,

Liu

et al. 2024

Preprint

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Dual actions of chloroinconazide on pepper blight in Capsicum annuum: disruption of Phytophthora capsici mycelium and activation of CaCNGC9‐mediated SA signaling

Zhu,

Zou,

Liao

et al. 2024

Pest Management Science

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BACKGROUNDPepper blight, caused by Phytophthora capsici, is a devastating disease that seriously threatens pepper production worldwide. With the emergence of resistance in P. capsici against conventional fungicides, there is an urgent need to explore novel alternatives for pepper blight management. This study aims to assess the inhibitory effect of chloroinconazide (CHI), a compound synthesized from tryptophan, against pepper blight, and to explore its potential mechanisms of action.RESULTSThe results demonstrated that CHI effectively targeted P. capsici, disrupting its growth and mycelial structure, which resulted in the release of dissolved intracellular substances. Additionally, CHI significantly inhibited the sporangium formation, zoospores release, and zoospores germination, thereby reducing the re‐infection of P. capsici. In contrast, the commercial pesticide methylaxyl only inhibited mycelial growth and had limited effect on re‐infection, while azoxystrobin inhibited re‐infection but had a weak inhibitory effect on mycelial growth. Furthermore, CHI activated the salicylic acid (SA) signaling pathway‐mediated immune response to inhibit P. capsici infection in pepper, with this activation being contingent upon cyclic nucleotide‐gated ion channel CaCNGC9.CONCLUSIONCHI exhibited potent dual inhibitory effects on P. capsici by disrupting mycelial structure and activating the CaCNGC9‐mediated SA signaling pathway. These dual mechanisms of action suggested that CHI could serve as a promising alternative chemical fungicide for the effective management of pepper blight, offering a new approach to control this devastating disease. Our findings highlighted the potential of CHI as a sustainable and efficient solution to combat the increasing resistance of P. capsici to conventional fungicides, ensuring better crop protection and yield. © 2024 Society of Chemical Industry.

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Bacillus velezensis BER1 enriched Flavobacterium daejeonense-like bacterium in the rhizosphere of tomato against bacterial wilt

Wang

Ding

Chen

et al. 2023

FEMS Microbiology Ecology

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Beneficial microorganisms can protect crop from phytopathogens, and modify rhizosphere microbiome. However, it is not well understood whether or how do rhizosphere microorganisms which respond to bio-agents contribute to disease suppression. Bacillus velezensis BER1 and tomato bacterial wilt caused by Ralstonia solanacearum were selected as models to disentangle the interactions and mechanisms in the rhizosphere. B. velezensis BER1 greatly suppressed tomato bacterial wilt by over 49.0%, reduced R. solanacearum colonization in the rhizosphere by 36.3%, and significantly enriched two Flavobacterium ASVs (1357 and 2401). A novel colony Loop-Mediated Isothermal Amplification (LAMP) assay system was developed to screen out Flavobacterium from tomato rhizosphere bacterial isolates. In vitro tests revealed that co-cultivating BER1 with Flavobacterium C45 increased biofilm formation by 18.6%. Climate chamber experiment further revealed that Flavobacterium C45 improved the control efficiency of BER1 on tomato bacterial wilt by 46.0%, decreased the colonization of R. solanacearum in the rhizosphere by 43.1% and elevated the transcription of plant defense gene PR1α in tomato by 45.4%. In summary, Flavobacterium C45 boosted the ability of B. velezensis BER1 to prevent bacterial wilt and the colonization of R. solanacearum, highlighting the importance of helper bacteria on elevating the efficiency of biological control.

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RT-qPCR Analysis of Host Defense-Related Genes in Nonhost Resistance: Wheat-Bgh Interaction

Cited by 4 publications

References 35 publications

The Impact of Biocontrol Bacteria on Cotton Resistance and Their Effects on Signaling Pathways Related to Defense Against Verticillium Wilt Infection

The Impact of Biocontrol Bacteria on Cotton Resistance and Their Effects on Signaling Pathways Related to Defense Against Verticillium Wilt Infection

Dual actions of chloroinconazide on pepper blight in Capsicum annuum: disruption of Phytophthora capsici mycelium and activation of CaCNGC9‐mediated SA signaling

Bacillus velezensis BER1 enriched Flavobacterium daejeonense-like bacterium in the rhizosphere of tomato against bacterial wilt

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