RT‐PCR Topography of Chronic Psoriasis Skin Based on Analysis of T‐Cell Receptor B Variable Region Gene Usage

Abstract

Psoriasis is a hyperproliferative inflammatory disease and 70% of patients develop a chronic plaque form. The pathogenesis of psoriasis is not known but evidence exists that T cells play a crucial role. The T cell V-gene receptor repertoire from psoriasis skin (different layers) was compared with peripheral blood T cells by employing RNA polymerase chain reaction (PCR) amplification. T cell receptor ( TCR) BV 5.1, 11, 12, 13.1 and 16 were utilized to a significantly higher degree in areas close to the basal layers when compared to CD4+, CD8+ or unfractionated blood T cells from the same patients, whereas only BV11 and 13.1 genes of T cells from deeper layers of the dermis showed such a skewed usage. No biased usage of TCRBV genes was observed in superficial layers or in whole skin. Furthermore, T cell receptor junctional diversity analysed by high resolution gel electrophoresis showed skin psoriatic T cells to be poly-or oligoclonal. In conclusion, we show that TCRBV gene usage from different layers of psoriatic skin has a different pattern compared with the corresponding gene usage in circulating peripheral blood T cells. This pattern may implicate possible skinassociated antigen or superantigens activating a limited number of T cells in areas of skin close to basal layers, which in turn could promote keratinocyte proliferation.

Psoriasis – as an autoimmune disease caused by molecular mimicry

“…Moreover, identical T cell clones have been detected in both skin and synovial lesions in patients with psoriatic arthritis (PsA) [31,32]. Notably, in the four studies where CD4 + and CD8 + T cells in the blood were analyzed separately, oligoclonality was detected among the CD8 + but not the CD4 + T cells [25,26,33,34].…”

HLA-Cw and TCR vβ analysis in twenty Mexican patients with psoriasis

“…This inconsistency has been reported by other authors, [20] and could be explained by the event referred to as the Koebner response [12]. Previous studies have reported selectivity of the clones displaying different TCR Vβ of infiltrated T-cells in psoriatic lesions: TCR Vβ-13 and -15 [11], TCR Vβ-5.1, -11, -12, -13.1 and -16 [21], TCR Vβ-3 and -13.1 [10], and more recently TCR Vβ-3, -13S2 and -21 [22]. This evidence is bound to studies of patient specimens with streptococcal infections.…”

New Discernment of Pathophysiological Aspects of Multiple Sclerosis Based On Mono Amino Oxidase (MAO) and Ion Channel Receptor 5HT3RA as Activator of T-Cells

“…There is other study indicating overexpression of this receptor in certain diseases, including the study of Ahangari et al [18] in which the expression of this receptor was increased in patients with asthma and breast cancer. In addition, in a study conducted by Saberi et al [20] overexpression of this receptor was indicated in lupus patients. In contrast, in a study in patients with schizophrenia and Tardive dyskinesia, it was found that the expression of this receptor in these diseases is reduced compared to healthy controls.…”

“…Samples transferred to laboratory in flask. Peripheral blood mononuclear cells isolated from 4 ml of whole blood each sample based on the gradient density centrifugation technique via Ficoll-hypaque [20]. Then the cells in phosphate buffered saline (PBS) were washed and cell viability was determined by trypan blue staining and concentration of cells was normalized (Concentration 7 × 10 6 cells/ml).the percentage of viable cells was more than 99%.…”