RT-PCR evaluation for identification and sequence analysis of foot-and-mouth disease serotype O from 2006 to 2007 in Punjab, Pakistan

Saeed, Ali; Khan, Qaiser M.; Waheed, Usman; Arshad, Memoona; Asif, Muhammad; Farooq, Muhammad

doi:10.1016/j.cimid.2009.10.004

Cited by 19 publications

(16 citation statements)

References 17 publications

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“…In the region, other subtypes circulated; in India, not only serotype O (Mason et al., 2003; Saeed et al., 2009) but also serotype C (Nagendrakumar et al., 2005) and serotype A (Nagendrakumar et al., 2009) have been isolated with both O and A isolated frequently and recently. Serotype Asia 1 has also been identified in India (Valarcher et al., 2009).…”

Section: Resultsmentioning

confidence: 99%

Molecular Characterization of Foot-and-Mouth Disease Virus: Implications for Disease Control in Bangladesh

Loth¹,

Osmani²,

Kalam³

et al. 2011

Transboundary and Emerging Diseases

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Foot-and-mouth disease (FMD) is endemic in Bangladesh, and to implement an effective FMD control programme, it is essential to understand the complex epidemiology of the disease. Here, we report on the characterization of FMD virus (FMDV) recovered from FMD outbreaks in Bangladesh in late 2009. All isolated viruses belonged to the FMDV serotype O. The phylogenetic reconstruction showed that all isolates belonged to the Middle East-South Asia (ME-SA) topotype, but fell into two distinct sublineages, one named Ind-2001 (the other has not been named). Within both sublineages, the 2009 Bangladesh isolates were most closely related to viruses from Nepal collected during 2008 and 2009. Additionally, both sublineages contained older viruses from India collected in 2000 and 2001. In South Asia, there is extensive cross-border cattle movement from Nepal and India to Bangladesh. Both these findings have implications for the control of FMD in Bangladesh. Because of the porous borders, a regional FMD control strategy should be developed. Further, animal identification and monitoring animal movements are necessary to identify the cross-border movements and market chain interactions of ruminants, leading to improved border and movement controls. Additionally, a vaccination strategy should be developed with the initial objective of protecting small-scale dairy herds from disease. For any successful FMD control programme, long-term Government commitment and adequate resources are necessary. A sustainable programme will also need farmer education, commitment and financial contributions.

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Section: Resultsmentioning

confidence: 99%

Molecular Characterization of Foot-and-Mouth Disease Virus: Implications for Disease Control in Bangladesh

Loth¹,

Osmani²,

Kalam³

et al. 2011

Transboundary and Emerging Diseases

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“…Detection of FMDV PCR has been described , 2015; Hossen et al, 2014;Loth et al, 2011;Saeed et al, 2011). We have performed PCR after synthesis of cDNA from the extracted RNA of the FMD virus and found positive bands at 866bp after electrophoresis in 2% agarose gel which was specific for FMDV serotype "A" (Figure 2 Present study proved that FMDV serotype "A" is present in the Pabna district of Bangladesh.…”

mentioning

confidence: 93%

RT-PCR based detection and adaptation of foot and mouth disease virus serotype A in BHK-21 cell line

et al. 2016

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The economic consequences of an outbreak of Foot and Mouth Disease (FMD) in Bangladesh is devastating. A sensitive, reliable and rapid diagnosis is crucial for the effective control of FMD. The present research was conducted for quick molecular detection and adaptation of FMD virus (FMDV) in BHK-21 (Baby Hamster Kidney) cell line. A two-step RT-PCR method was applied for the detection of the FMDV. Without prior adaption into BHK-21 cell culture, it is difficult to detect FMDV directly from the field samples by RT-PCR method. Samples were collected from the tongue epithelium (N=19) and inter digital tissues (N=4) of the suspected animals in Sujanagar, Bera, Santhia of Pabna district and Kotowali, Trishal of Mymensingh districts of Bangladesh during the year 2014. BHK-21 cell subculture was done from a previously cultured bottle containing BHK-21 cells. Prepared inocula were inoculated into BHK-21 cell culture and incubated at 37ºC for 24 h. After 36 h, cytopathic effects (CPE) were observed in BHK-21 cell line characterized by rounding and flattening of the cells, multinucleated giant cells formation, breaking down of the intracellular bridges and finally cell death indicated the presence of FMDV. Clear infectious BHK-21 cell culture fluid was collected and preserved at -20ºC temperature for virus detection by RT-PCR with serotype specific primers. Viral RNA was extracted from the clear infectious cell culture fluid for cDNA synthesis and used for PCR. Out of 23 samples tested, 3(13.04%) were positive for FMDV serotype A. The findings of this study can be helpful for the selection of vaccine having specific FMDV type, and it may help in controlling FMD in Bangladesh.Progressive Agriculture 27 (1): 64-69, 2016

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“…Однако, на сегодняшний день синдром папулёзно-геморрагической сыпи в виде перчаток и носков больше всего характерен для энтеровирусной группы инфекций (ECHO (5, 9, 17, 22) и Коксаки А16) и развивается довольно часто. Наибольшую распространенность заболевания, ассоциированного с энтеровирусами, отмечают в Южной Азии [10]. Известно, что чаще болеют дети дошкольного возраста.…”

Section: результаты и их обсуждениеunclassified

“…Характерна цикличность данного инфекционного процесса с подъемами заболеваемости каждые 3-5 лет. При наличии только кожного синдрома специфическая вирусологическая диагностика не требуется [9,10].…”

Section: результаты и их обсуждениеunclassified

Rare Forms of Exanthems in Pediatric Clinical Practice

2018

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The infant roseola, viral pemphigus, papular-hemorrhagic rash as a form of gloves and socks syndrome, and one-sided laterothoracic exanthema can be referred to the rare types of infectious rash in the pediatrician’s practice. These diseases, despite certain signs peculiar to everyone, are often not diagnosed during the initial admission to a pediatrician. The study describes the characteristics of each type of rash, the course of the disease, and discusses possible errors in the primary diagnosis of rare infectious rashes.

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RT-PCR evaluation for identification and sequence analysis of foot-and-mouth disease serotype O from 2006 to 2007 in Punjab, Pakistan

Cited by 19 publications

References 17 publications

Molecular Characterization of Foot-and-Mouth Disease Virus: Implications for Disease Control in Bangladesh

Molecular Characterization of Foot-and-Mouth Disease Virus: Implications for Disease Control in Bangladesh

RT-PCR based detection and adaptation of foot and mouth disease virus serotype A in BHK-21 cell line

Rare Forms of Exanthems in Pediatric Clinical Practice

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RT-PCR evaluation for identification and sequence analysis of foot-and-mouth disease serotype O from 2006 to 2007 in Punjab, Pakistan

Cited by 19 publications

References 17 publications

Molecular Characterization of Foot-and-Mouth Disease Virus: Implications for Disease Control in Bangladesh

Molecular Characterization of Foot-and-Mouth Disease Virus: Implications for Disease Control in Bangladesh

RT-PCR based detection and adaptation of foot and mouth disease virus serotype A in BHK-21 cell line

Rare Forms of Exanthems in Pediatric Clinical Practice

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RT-PCR based detection and adaptation of foot and mouth disease virus serotype A in BHK-21 cell line