Rt-PCR Detection of Cytokine Transcripts in a Series of Cultured Human Meningiomas

Boyle-Walsh, Eilis; Birch, Mark; Gallagher, James A.; Speirs, Valerie; White, Michael C.; Shenkin, Alan; Fraser, William D.

doi:10.1002/(sici)1096-9896(199604)178:4<442::aid-path521>3.0.co;2-i

Cited by 12 publications

(2 citation statements)

References 16 publications

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“…Numerous studies have demonstrated that interleukin-1 beta (IL-1b), a pro-inflammatory cytokine in chronic inflammation, has a higher level in several tumor entities, including meningiomas, and plays necessary roles in tumor growth, metastasis, and angiogenesis [10][11][12][13]. Clinical studies have also shown that patients with IL-1b positive tumors had generally worse prognoses [14].…”

Section: Introductionmentioning

confidence: 99%

The role and regulatory mechanism of IL‐1β on the methylation of the NF2 gene in benign meningiomas and leptomeninges

Wang

Cui

Zhong

et al. 2016

Molecular Carcinogenesis

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Methylation of the neurofibromatosis type 2 (NF2) gene in low-grade meningioma (WHO grade I) has crucial roles in tumorigenesis and development. Meningioma formation might also occur in the setting of an inflammatory microenvironment. However, the association between inflammation and the methylation of NF2 remains unclear. The present study investigates the role and regulatory mechanism of IL-1β, one of the most important pro-inflammatory cytokines, in the methylation of NF2 in benign meningioma. Three primary low-grade meningioma cells and leptomeningeal cells were cultured. CCK-8 and BrdU assays demonstrated that proliferation of meningioma/leptomeningeal cells treated with IL-1β occurred in a dose- and time-dependent manner. Methylation-specific PCR verified that IL-1β induced methylation of the NF2 promoter and decreased NF2/merlin expression in meningioma/leptomeningeal cells. Real-time PCR, western blotting, and immunofluorescence showed that IL-1β up-regulated DNMT1 in meningioma cells and DNMT1/3b in leptomeningeal cells but did not up-regulate DNMT3a. After co-treatment with the DNMT inhibitor 5-Aza-2'-deoxycytidine and DNMT siRNA, methylation of NF2 induced by IL-1β was attenuated and merlin expression was restored. Furthermore, we showed that DNMT1 in meningiomas and DNMT1/3b in leptomeninges were regulated via activation of the MAPK (p38, ERK, JNK) and NF-κB pathways. These results suggest that IL-1β induces methylation of NF2 by up-regulating DNMT1 in benign meningioma cells and DNMT1/3b in leptomeningeal cells via MAPK and NF-κB pathways. Therefore, NF2 methylation is a linker between IL-1β and tumor development, and DNMTs might be potential therapeutic targets in meningioma for regulating NF2 and inhibiting tumor development. © 2016 Wiley Periodicals, Inc.

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Section: Introductionmentioning

confidence: 99%

The role and regulatory mechanism of IL‐1β on the methylation of the NF2 gene in benign meningiomas and leptomeninges

Wang

Cui

Zhong

et al. 2016

Molecular Carcinogenesis

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“…However, there is a lack of definitive research evidence regarding the association between TNF-beta and the prognosis of meningioma patients. Some studies have shown higher levels of TNF-beta in the peripheral blood of meningioma patients compared to normal individuals ( Boyle-Walsh et al, 1996 ), which may be associated with increased tumor invasiveness, higher risk of recurrence, and enhanced resistance to treatment. Increased expression of CXCL1 is associated with tumor development and progression in meningiomas.…”

Section: Discussionmentioning

confidence: 99%

Inflammatory factors and risk of meningiomas: a bidirectional mendelian-randomization study

et al. 2023

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BackgroundMeningiomas are one of the most common intracranial tumors, and the current understanding of meningioma pathology is still incomplete. Inflammatory factors play an important role in the pathophysiology of meningioma, but the causal relationship between inflammatory factors and meningioma is still unclear.MethodMendelian randomization (MR) is an effective statistical method for reducing bias based on whole genome sequencing data. It’s a simple but powerful framework, that uses genetics to study aspects of human biology. Modern methods of MR make the process more robust by exploiting the many genetic variants that may exist for a given hypothesis. In this paper, MR is applied to understand the causal relationship between exposure and disease outcome.ResultsThis research presents a comprehensive MR study to study the association of genetic inflammatory cytokines with meningioma. Based on the results of our MR analysis, which examines 41 cytokines in the largest GWAS datasets available, we were able to draw the relatively more reliable conclusion that elevated levels of circulating TNF-β, CXCL1, and lower levels of IL-9 were suggestive associated with a higher risk of meningioma. Moreover, Meningiomas could cause lower levels of interleukin-16 and higher levels of CXCL10 in the blood.ConclusionThese findings suggest that TNF-β, CXCL1, and IL-9 play an important role in the development of meningiomas. Meningiomas also affect the expression of cytokines such as IL-16 and CXCL10. Further studies are needed to determine whether these biomarkers can be used to prevent or treat meningiomas.

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LETTER TO THE EDITOR. RT-PCR detection of cytokine transcripts in a series of cultured human meningiomas

Robinson

Geddes

1997

J. Pathol.

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Rt-PCR Detection of Cytokine Transcripts in a Series of Cultured Human Meningiomas

Cited by 12 publications

References 16 publications

The role and regulatory mechanism of IL‐1β on the methylation of the NF2 gene in benign meningiomas and leptomeninges

The role and regulatory mechanism of IL‐1β on the methylation of the NF2 gene in benign meningiomas and leptomeninges

Inflammatory factors and risk of meningiomas: a bidirectional mendelian-randomization study

LETTER TO THE EDITOR. RT-PCR detection of cytokine transcripts in a series of cultured human meningiomas

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