RSOsPR10 Expression in Response to Environmental Stresses is Regulated Antagonistically by Jasmonate/Ethylene and Salicylic Acid Signaling Pathways in Rice Roots

Abstract

Plant roots play important roles not only in the absorption of water and nutrients, but also in stress tolerance. Previously, we identified RSOsPR10 as a root-specific pathogenesis-related (PR) protein induced by drought and salt treatments in rice. Transcripts and proteins of RSOsPR10 were strongly induced by jasmonate (JA) and the ethylene (ET) precursor 1-aminocyclopropane-1-carboxylic acid (ACC), while salicylic acid (SA) almost completely suppressed these inductions. Immunohistochemical analyses showed that RSOsPR10 strongly accumulated in cortex cells surrounding the vascular system of roots, and this accumulation was also suppressed when SA was applied simultaneously with stress or hormone treatments. In the JA-deficient mutant hebiba, RSOsPR10 expression was up-regulated by NaCl, wounding, drought and exogenous application of JA. This suggested the involvement of a signal transduction pathway that integrates JA and ET signals in plant defense responses. Expression of OsERF1, a transcription factor in the JA/ET pathway, was induced earlier than that of RSOsPR10 after salt, JA and ACC treatments. Simultaneous SA treatment strongly inhibited the induction of RSOsPR10 expression and, to a lesser extent, induction of OsERF1 expression. These results suggest that JA/ET and SA pathways function in the stress-responsive induction of RSOsPR10, and that OsERF1 may be one of the transcriptional factors in the JA/ET pathway.

RICE SALT SENSITIVE3 Forms a Ternary Complex with JAZ and Class-C bHLH Factors and Regulates Jasmonate-Induced Gene Expression and Root Cell Elongation

“…The link between this modulation and the RSS3-mediated regulation of JA-responsive genes remains unclear, although JAs are known to be involved in various stress responses (Wasternack, 2007;Takeuchi et al, 2011). Notably, Arabidopsis MYC2 was originally identified as a transcriptional activator of stress-responsive genes (Abe et al, 1997).…”

“…JAs are also involved in the responses to a variety of abiotic stresses, such as salinity and drought stress (Wasternack, 2007;Takeuchi et al 2011). In cultured Arabidopsis thaliana cells, JAs mediate the transcriptional reprogramming of genes, as reflected in the repression of the cell cycle and the biosynthesis of phenylpropanoids, including lignin precursors (Pauwels et al, 2008).…”

The Pathogenesis Related Class 10 proteins in Plant Defense against Biotic and Abiotic Stresses

“…Accumulations of JIOPR10 [73] and OsPR10 [74] transcripts were observed on application of JA and SA in rice leaves. The folding canon of PR-10 proteins is found in the ABA receptor family known as PYR/PYL/RCAR (pyrabactin resistance/PYR-like/regulatory component of ABA response) [21].…”

Overexpression of RSOsPR10, a root-specific rice PR10 gene, confers tolerance against drought stress in rice and drought and salt stresses in bentgrass

“…Subsequent studies revealed the involvement of the JA/ET signaling pathway in RSOsPR10 induction during responses to high salinity conditions and wounding, and the inhibition of expression by exogenous salicylic acid treatment (Takeuchi et al 2011). Immunohistochemical analyses indicated RSOsPR10 accumulated mainly in root cortical cells after stress exposure and treatments with JA and ET, suggesting it affects root growth in response to environmental stresses.…”