RSL Class II Transcription Factors Guide the Nuclear Localization of RHL1 to Regulate Root Hair Development

Moon, Sunok; Cho, Lae Hyeon; Kim, Yu Jin; Gho, Yun Shil; Jeong, Hee Jeong; Hong, Woo Jong; Lee, Chanhui; Park, Hyon; Jwa, Nam‐Soo; Dangol, Sarmina; Chen, Yafei; Park, Hayeong; Cho, Hyun Soo; An, Gynheung; Jung, Ki‐Hong

doi:10.1104/pp.18.01002

Cited by 27 publications

(14 citation statements)

References 49 publications

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“…Besides auxin, genetic factors play important roles in the development of RHs. RSL Class II family members are expressed in root epidermis and RH cells and as essential regulators of RH development in rice [34]. In this study, compared with WT, the expression of RSL4/5/6/9 were up-regulated in OE lines under control and LP condition (Figure 6).…”

Section: Discussionmentioning

confidence: 57%

Overexpression of OsPIN2 Regulates Root Growth and Formation in Response to Phosphate Deficiency in Rice

Sun

Guo

et al. 2019

IJMS

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The response of root architecture to phosphate (P) deficiency is critical in plant growth and development. Auxin is a key regulator of plant root growth in response to P deficiency, but the underlying mechanisms are unclear. In this study, phenotypic and genetic analyses were undertaken to explore the role of OsPIN2, an auxin efflux transporter, in regulating the growth and development of rice roots under normal nutrition condition (control) and low-phosphate condition (LP). Higher expression of OsPIN2 was observed in rice plants under LP compared to the control. Meanwhile, the auxin levels of roots were increased under LP relative to control condition in wild-type (WT) plants. Compared to WT plants, two overexpression (OE) lines had higher auxin levels in the roots under control and LP. LP led to increased seminal roots (SRs) length and the root hairs (RHs) density, but decreased lateral roots (LRs) density in WT plants. However, overexpression of OsPIN2 caused a loss of sensitivity in the root response to P deficiency. The OE lines had a shorter SR length, lower LR density, and greater RH density than WT plants under control. However, the LR and RH densities in the OE lines were similar to those in WT plants under LP. Compared to WT plants, overexpression of OsPIN2 had a shorter root length through decreased root cell elongation under control and LP. Surprisingly, overexpression of OsPIN2 might increase auxin distribution in epidermis of root, resulting in greater RH formation but less LR development in OE plants than in WT plants in the control condition but levels similar of these under LP. These results suggest that higher OsPIN2 expression regulates rice root growth and development maybe by changing auxin distribution in roots under LP condition.

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Section: Discussionmentioning

confidence: 57%

Overexpression of OsPIN2 Regulates Root Growth and Formation in Response to Phosphate Deficiency in Rice

Sun

Guo

et al. 2019

IJMS

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“…We measured root hairs length from the seminal roots as we previously described (Moon et al ., 2019b). Briefly, the root hairs were photographed with a BX61 microscope camera (Olympus, Tokyo, Japan) and more than 60 root hairs were quantified with analySIS LS Research 5.0 software.…”

Section: Methodsmentioning

confidence: 99%

CAFRI‐Rice: CRISPR applicable functional redundancy inspector to accelerate functional genomics in rice

Hong

Kim

et al. 2020

The Plant Journal

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Rice (Oryza sativa L.) is a staple crop with agricultural traits that have been intensively investigated. However, despite the variety of mutant population and multi-omics data that have been generated, rice functional genomic research has been bottlenecked due to the functional redundancy in the genome. This phenomenon has masked the phenotypes of knockout mutants by functional compensation and redundancy. Here, we present an intuitive tool, CRISPR applicable functional redundancy inspector to accelerate functional genomics in rice (CAFRI-Rice; cafri-rice.khu.ac.kr). To create this tool, we generated a phylogenetic heatmap that can estimate the similarity between protein sequences and expression patterns, based on 2,617 phylogenetic trees and eight tissue RNA-sequencing datasets. In this study, 33,483 genes were sorted into 2,617 families, and about 24,980 genes were tested for functional redundancy using a phylogenetic heatmap approach. It was predicted that 7,075 genes would have functional redundancy, according to the threshold value validated by an analysis of 111 known genes functionally characterized using knockout mutants and 5,170 duplicated genes. In addition, our analysis demonstrated that an anther/pollen-preferred gene cluster has more functional redundancy than other clusters. Finally, we showed the usefulness of the CAFRI-Rice-based approach by overcoming the functional redundancy between two root-preferred genes via loss-of-function analyses as well as confirming the functional dominancy of three genes through a literature search. This CAFRI-Rice-based target selection for CRISPR/Cas9-mediated mutagenesis will not only accelerate functional genomic studies in rice but can also be straightforwardly expanded to other plant species.

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“…However, fundamental players like RSL genes work in a similar way. Both RSL class I and class II genes are known in rice and other species that positively regulate root hair development (Y. Han et al, 2016; Kim et al, 2017; Moon et al, 2019). Further, various mutant screens have identified many root hair developmental genes.…”

Section: Evolution Of Rsl Family Genes and Regulation In Controlling ...mentioning

confidence: 99%

Significance of root hairs in developing stress‐resilient plants for sustainable crop production

Kohli

Maurya

Thakur

et al. 2021

Plant Cell & Environment

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Root hairs represent a beneficial agronomic trait to potentially reduce fertilizer and irrigation inputs. Over the past decades, research in the plant model Arabidopsis thaliana has provided insights into root hair development, the underlying genetic framework and the integration of environmental cues within this framework. Recent years have seen a paradigm shift, where studies are now highlighting conservation and diversification of root hair developmental programs in other plant species and the agronomic relevance of root hairs in a wider ecological context. In this review, we specifically discuss the molecular evolution of the RSL (RHD Six‐Like) pathway that controls root hair development and growth in land plants. We also discuss how root hairs contribute to plant performance as an active physiological rooting structure by performing resource acquisition, providing anchorage and constructing the rhizosphere with desirable physical, chemical and biological properties. Finally, we outline future research directions that can help achieve the potential of root hairs in developing sustainable agroecosystems.

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RSL Class II Transcription Factors Guide the Nuclear Localization of RHL1 to Regulate Root Hair Development

Cited by 27 publications

References 49 publications

Overexpression of OsPIN2 Regulates Root Growth and Formation in Response to Phosphate Deficiency in Rice

Overexpression of OsPIN2 Regulates Root Growth and Formation in Response to Phosphate Deficiency in Rice

CAFRI‐Rice: CRISPR applicable functional redundancy inspector to accelerate functional genomics in rice

Significance of root hairs in developing stress‐resilient plants for sustainable crop production

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