rRNA gene restriction patterns as a characterization tool for Lactobacillus sake strains producing ropy slime

Abstract

The rRNA gene restriction patterns (ribotypes) of 69 ropy slime producing Lactobacillus sake strains isolated mainly from vacuumpackaged meat products of ten meat plants were determined. Ribotypes of these spoilage bacteria were compared to the corresponding patterns of non-ropy L. sake strains, and also to other species of the sake strains but the patterns alone cannot be used as markers of slime production capability. Comparison of ribotypes between different species of the genus Lactobacillus suggest that ribotyping may also be a suitable method for species identification within the genus Lactobacillus.

Characterisation of lactic acid bacteria from spoiled, vacuum-packaged, cold-smoked rainbow trout using ribotyping

“…When fish products have been studied, phenotyping of LAB has resulted in unreliable species identification. LAB originating from vacuum-packaged, salted, smoked herring gave atypical profiles (Gancel et al, 1997), and in a study of the spoilage flora of vacuum-packaged, salted, cold-smoked rainbow trout fillets (Lyhs et al, 1998), we also concluded that phenotyping is not always reliable for Several molecular methods have been used for strain typing or characterisation of LAB (Hill and Hill, 1986;Tannock et al, 1990;Welsh and McClelland, 1990;Williams et al, 1990;Duffner et al, 1993;Dykes et al, 1993;Jaho et al, 1993;Roussel et al, 1993;Björkroth et al, 1996aBjörkroth et al, , 1996b, but only some of these applications have been used for establishing species identification libraries. Paludan-Müller et al (1998), studying the role of Carnobacterium species in the spoilage of vacuum-packaged, cold-smoked salmon, used whole-cell-protein patterns for identification of the carnobacteria.…”

Streptococcus parauberis associated with modified atmosphere packaged broiler meat products and air samples from a poultry meat processing plant

“…The Streptococcus parauberis type and reference strains used were LMG 12174 T and its duplicate LMG 14376 T , LMG 12173 R and its duplicate LMG 14377 R . In addition, strains RM212.1 and RA149.1, isolated from diseased turbots (Romalde et al, 1999) DNA-based analyses was isolated as previously described by Björkroth and Korkeala (1996).…”

Lactobacillus alimentarius: a specific spoilage organism in marinated herring

“…Two isolates from each jar of herring were further subjected to ribotyping. HindIII was chosen because it has been found to provide species-specific patterns for spoilage LAB (Björkroth and Korkeala, 1996;Björkroth et al, 1998Björkroth et al, , 2000.…”

“…DNA was isolated according to the guanidium thiocyanate method of Pitcher et al (1989), modified by Björkroth and Korkeala (1996) with combined mutanolysin (Sigma Chemical Company, St. Louis, MO, USA) and lysozyme (Sigma) treatment.…”

“…REA, southern transfer, hybridisation and the cDNA probe for rRNA gene restriction patterns (ribotypes) were prepared as described by Björkroth and Korkeala (1996). …”