RPS27, a sORF-Encoded Polypeptide, Functions Antivirally by Activating the NF-κB Pathway and Interacting With Viral Envelope Proteins in Shrimp

Diao, Meng-Qi; Li, Cang; Xu, Jie; Zhao, Xiao‐Fan; Wang, Jin-Xing

doi:10.3389/fimmu.2019.02763

Cited by 18 publications

(6 citation statements)

References 48 publications

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“…Human RPS27 is required for NF-κB phosphorylation and nuclear translocation [243]. RPS27 also regulates NF-κB signaling in shrimp [244]. Human RPS3A stimulates NF-κB nuclear translocation synergistically with hepatitis B virus X protein (HBx) [245].…”

Section: Roles Of Ctas In Transcriptional Regulationmentioning

confidence: 99%

Localization and Functional Roles of Components of the Translation Apparatus in the Eukaryotic Cell Nucleus

Kachaev

Ivashchenko

Kozlov

et al. 2021

Cells

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Components of the translation apparatus, including ribosomal proteins, have been found in cell nuclei in various organisms. Components of the translation apparatus are involved in various nuclear processes, particularly those associated with genome integrity control and the nuclear stages of gene expression, such as transcription, mRNA processing, and mRNA export. Components of the translation apparatus control intranuclear trafficking; the nuclear import and export of RNA and proteins; and regulate the activity, stability, and functional recruitment of nuclear proteins. The nuclear translocation of these components is often involved in the cell response to stimulation and stress, in addition to playing critical roles in oncogenesis and viral infection. Many components of the translation apparatus are moonlighting proteins, involved in integral cell stress response and coupling of gene expression subprocesses. Thus, this phenomenon represents a significant interest for both basic and applied molecular biology. Here, we provide an overview of the current data regarding the molecular functions of translation factors and ribosomal proteins in the cell nucleus.

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Section: Roles Of Ctas In Transcriptional Regulationmentioning

confidence: 99%

Localization and Functional Roles of Components of the Translation Apparatus in the Eukaryotic Cell Nucleus

Kachaev

Ivashchenko

Kozlov

et al. 2021

Cells

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“…These two sequences are not coding regions in the genome of SARS-CoV-2, as they are not in the coding reading frame of S gene. However, in the light of this significant evidence [ 24 ] and of the increasing importance of small open-reading frame (sORF)-encoded peptides (SEPs) [ 27 , 28 , 29 , 30 ], recurrent especially in microorganisms [ 31 , 32 , 33 ], we investigated whether the translation of these highly conserved base-pairing regions could give compounds with antiviral activity. We found that the two tripeptides are able to inhibit betacoronavirus entry mechanism by binding the RBD of SARS-CoV-2 Spike protein, as suggested by molecular docking and validated by biochemical studies.…”

Section: Introductionmentioning

confidence: 99%

Design of Three Residues Peptides against SARS-CoV-2 Infection

Zannella

Chianese

Greco

et al. 2022

Viruses

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The continuous and rapid spread of the COVID-19 pandemic has emphasized the need to seek new therapeutic and prophylactic treatments. Peptide inhibitors are a valid alternative approach for the treatment of emerging viral infections, mainly due to their low toxicity and high efficiency. Recently, two small nucleotide signatures were identified in the genome of some members of the Coronaviridae family and many other human pathogens. In this study, we investigated whether the corresponding amino acid sequences of such nucleotide sequences could have effects on the viral infection of two representative human coronaviruses: HCoV-OC43 and SARS-CoV-2. Our results showed that the synthetic peptides analyzed inhibit the infection of both coronaviruses in a dose-dependent manner by binding the RBD of the Spike protein, as suggested by molecular docking and validated by biochemical studies. The peptides tested do not provide toxicity on cultured cells or human erythrocytes and are resistant to human serum proteases, indicating that they may be very promising antiviral peptides.

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“…The healthy shrimp M. japonicus (8–10 g) were purchased from a shrimp farm near Qingdao, Shandong Province, China. A shrimp culture system in the laboratory was used to cultivate the shrimp ( 40 ). For tissue distribution analysis, different organs, including the heart, hepatopancreas, gill, intestines, and stomach, were dissected from shrimp for protein and total RNA extraction.…”

Section: Methodsmentioning

confidence: 99%

Nitric Oxide Synthase Regulates Gut Microbiota Homeostasis by ERK-NF-κB Pathway in Shrimp

et al. 2021

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The gut microbiota is a complex group of microorganisms that is not only closely related to intestinal immunity but also affects the whole immune system of the body. Antimicrobial peptides and reactive oxygen species participate in the regulation of gut microbiota homeostasis in invertebrates. However, it is unclear whether nitric oxide, as a key mediator of immunity that plays important roles in antipathogen activity and immune regulation, participates in the regulation of gut microbiota homeostasis. In this study, we identified a nitric oxide synthase responsible for NO production in the shrimp Marsupenaeus japonicus. The expression of Nos and the NO concentration in the gastrointestinal tract were increased significantly in shrimp orally infected with Vibrio anguillarum. After RNA interference of Nos or treatment with an inhibitor of NOS, L-NMMA, NO production decreased and the gut bacterial load increased significantly in shrimp. Treatment with the NO donor, sodium nitroprusside, increased the NO level and reduced the bacterial load significantly in the shrimp gastrointestinal tract. Mechanistically, V. anguillarum infection increased NO level via upregulation of NOS and induced phosphorylation of ERK. The activated ERK phosphorylated the NF-κB-like transcription factor, dorsal, and caused nuclear translocation of dorsal to increase expression of antimicrobial peptides (AMPs) responsible for bacterial clearance. In summary, as a signaling molecule, NOS-produced NO regulates intestinal microbiota homeostasis by promoting AMP expression against infected pathogens via the ERK-dorsal pathway in shrimp.

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RPS27, a sORF-Encoded Polypeptide, Functions Antivirally by Activating the NF-κB Pathway and Interacting With Viral Envelope Proteins in Shrimp

Cited by 18 publications

References 48 publications

Localization and Functional Roles of Components of the Translation Apparatus in the Eukaryotic Cell Nucleus

Localization and Functional Roles of Components of the Translation Apparatus in the Eukaryotic Cell Nucleus

Design of Three Residues Peptides against SARS-CoV-2 Infection

Nitric Oxide Synthase Regulates Gut Microbiota Homeostasis by ERK-NF-κB Pathway in Shrimp

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