RPS2 of
            <i>Arabidopsis thaliana</i>
            : a Leucine-Rich Repeat Class of Plant Disease Resistance Genes

Bent, Andrew F.; Kunkel, Barbara N.; Dahlbeck, Douglas; Brown, Kenneth E.; Schmidt, Renate; Giraudat, Jérôme; Leung, Jeffrey; Staskawicz, Brian J.

doi:10.1126/science.8091210

Cited by 875 publications

(540 citation statements)

References 36 publications

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“…This insert was ligated into the SalI, SmaI sites of pBINAR-Hyg. The two constructs were introduced into Agrobacterium tumefaciens C58C1 and used to transform Arabidopsis Col-2 plants via vacuum infiltration (Bechtold et al, 1993;Bent et al, 1994). Transformed plants (T 1 generation) were selected on MS medium containing 1% (w/v) sucrose and 25 µg ml -1 hygromycin B (Calbiochem, La Jolla, CA, USA).…”

Section: Plant Transformationmentioning

confidence: 99%

The role of UDP‐glucose epimerase in carbohydrate metabolism of Arabidopsis

Dörmann

Benning

1998

The Plant Journal

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SummaryUridine 5Ј-diphospho-glucose-4-epimerase (UDP-Glc epimerase) catalyses the reversible epimerization of UDPgalactose and UDP-glucose. In contrast to bacteria and yeast, expression of the UDP-Glc epimerase gene in Arabidopsis was found not to be induced by galactose. To elucidate the metabolic role of this enzyme, transgenic Arabidopsis plants expressing the respective cDNA in sense or antisense orientation were constructed, leading to a range of plant lines with different UDP-Glc epimerase activities. No alterations in morphology were observed and the relative amounts of different galactose-containing compounds were not affected if the plants were raised on soil. However, on agar plates in the presence of galactose, the growth of different lines was increasingly repressed with decreasing enzyme activity, and an increase in the UDP-Gal content was observed in parallel, whereas the UDP-Glc content was nearly constant. The amount of galactose in the cell wall was increased in plants with low UDP-Glc epimerase activity grown on galactose, whereas the cellulose content in the leaves was not altered. Furthermore, starch determined at different times of the day was highly abundant in plants with low UDP-Glc epimerase activity in the presence of galactose. It is proposed that low endogenous UDP-Glc epimerase activity is responsible for the galactose toxicity of the wild-type. Possible mechanisms by which the starch content might be modulated are discussed.

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Section: Plant Transformationmentioning

confidence: 99%

The role of UDP‐glucose epimerase in carbohydrate metabolism of Arabidopsis

Dörmann

Benning

1998

The Plant Journal

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“…The discovery of the RXC3 gene encoding for Xanthomonas recognition/reaction extends the number of pathogen specificities located within this interval. Cloning and characResistance in Arabidopsis to X. co campestris 27 terization of RXC3 will allow comparison of a digenic resistance gene not only to other recently cloned resistance genes (Bent et al, 1994;Grant et al, 1995;Jones et al, 1994;Lawrence et aL, 1995;Mindrinos et aL, 1994;Song et al, 1995;Whitham et al, 1994), but also to other resistance genes located in the MRC-J interval (Lee et aL, 1996;McDowell et al, 1996).…”

Section: Discussionmentioning

confidence: 99%

“…RPS2 and RPM1/RPS3, which confer resistance to Pseudomonas syringae pathovars, encode proteins with a leucine-rich repeat domain (Bent et al, 1994;Grant et al, 1995;Mindrinos et al, 1994). This feature is conserved in several other disease resistance genes, such as the Cfo9, N, L e and Xa21 genes cloned from other angiosperms, indicating a conservation in resistance mechanisms to bacterial, viral and fungal pathogens in flowering plants (Jones et al, 1994;Lawrence et al, 1995;Song et aL, 1995;Whitham et aL, 1994).…”

Section: Introductionmentioning

confidence: 99%

“…The advantages of Arabidopsis thaliana as a model for plant pathology studies have resulted in the identification and mapping of loci conferring resistance to bacterial, fungal, and viral pathogens as well as the cloning of the RPS2 and the RPM1/RPS3 resistance genes (Bent et al, 1994;Grant et aL, 1995;Kunkel, 1996;Mindrinos et al, 1994). RPS2 and RPM1/RPS3, which confer resistance to Pseudomonas syringae pathovars, encode proteins with a leucine-rich repeat domain (Bent et al, 1994;Grant et al, 1995;Mindrinos et al, 1994).…”

Section: Introductionmentioning

confidence: 99%

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Use of Arabidopsis recombinant inbred lines reveals a monogenic and a novel digenic resistance mechanism to Xanthomonas campestris pv campestris

Buell

Somerville

1997

The Plant Journal

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SummaryInfiltration of the Arabidopsis thaliana accession Landsberg erecta (Ler) with Xanthomonas campestris pv campestris isolate 2D520 results in extensive necrosis and limited chlorosis within 5-6 days post-inoculation (d.p.i.}, which can lead to systemic necrosis within 23 d.p.i. In contrast, the accession Columbia (Col) remains asymptomatic after infiltration. Although both accessions support bacterial growth, 5-28-fold more bacteria are present in Let than in Col leaf tissue. Inheritance studies indicate that three independent, dominant or partially dominant, nuclear genes condition resistance to X. c. campestris 2D520. The major gene, termed RXC2, conditions monogenic resistance to X. c. campestris and was mapped to a 5.5 cM interval of chromosome V. Segregation data indicate that the locus RXC3 in conjunction with RXC4 confers digenic resistance to X. c. campestris. The combined action of RXC3 and RXC4 is correlated with a suppression of in planta bacterial levels and a suppression of symptoms relative to Ler. The RXC3 + RXC4-mediated resistance is novel in that although the Col allele of RXC4 contributes positively to resistance, it is the Ler and not the Col allele of RXC3 that contributes positively to resistance. RXC3 was mapped to the bottom arm of chromosome V in a 2.7 cM interval within the major recognition gene complex MRC-J, a cluster of genes involved in disease resistance. RXC4 was mapped to a 12 cM interval on chromosome II that also contains RXCl, a gene conferring tolerance to X. c. campestris.

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“…Examples of members of plant resistance genes from NBS-LRR gene family are Prf, I2CI and Mi from tomato [18], [19], [20], N from tobacco [21], RPM1 from Arabidopsis [22], M and L6 from flax [23], [24], RGC2 from lettuce [25], Xa1 from rice [26] and Cre3 from wheat [27].…”

Section: Introductionmentioning

confidence: 99%