RP-HPLC method development for the simultaneous determination of timolol maleate and human serum albumin in albumin nanoparticles

Boiero, Carolina; Allemandi, Daniel Alberto; Longhi, Marcela R.; Llabot, Juan Manuel

doi:10.1016/j.jpba.2015.03.031

Cited by 22 publications

(7 citation statements)

References 11 publications

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“…The inset of Figure 3 B showed that the fluorescence intensity had a linear relationship (R 2 = 0.9976) with HSA concentration in the concentration range of 0.15–2.5 μM, and the regression equation was Y = −313.35 X + 1064.3, where Y was the fluorescence intensity at 610 nm and X was the HA concentration. The limit of detection (LOD) of the proposed strategy was estimated to be 0.082 μM, which was lower and more rapid than the other methods [ 38 , 39 , 40 ]. Therefore, a simple, rapid, effective and sensitive method of HA determination was established.…”

Section: Resultsmentioning

confidence: 94%

A Novel Detection Method of Human Serum Albumin Based on the Poly(Thymine)-Templated Copper Nanoparticles

Chen

Xiang

et al. 2017

Sensors

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In this work, we developed a facile fluorescence method for quantitative detection of human serum albumin (HSA) based on the inhibition of poly(thymine) (poly T)-templated copper nanoparticles (CuNPs) in the presence of HSA. Under normal circumstances, poly T-templated CuNPs can display strong fluorescence with excitation/emission peaks at 340/610 nm. However, in the presence of HSA, it will absorb cupric ion, which will prevent the formation of CuNPs. As a result, the fluorescence intensity will become obviously lower in the presence of HSA. The analyte HSA concentration had a proportional linear relationship with the fluorescence intensity of CuNPs. The detection limit for HSA was 8.2 × 10−8 mol·L−1. Furthermore, it was also successfully employed to determine HSA in biological samples. Thus, this method has potential applications in point-of-care medical diagnosis and biomedical research.

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Section: Resultsmentioning

confidence: 94%

A Novel Detection Method of Human Serum Albumin Based on the Poly(Thymine)-Templated Copper Nanoparticles

Chen

Xiang

et al. 2017

Sensors

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“…The TM high-performance-liquid-chromatography (HPLC) determination was performed as described by Boiero et al [56] with slight modifications. The HPLC system was composed by a separation module Waters Alliance 2695 and a Waters photodiode array 2996 (Barcelona, Spain).…”

Section: Tm Hplc Determinationmentioning

confidence: 99%

Gelatin Nanoparticles-HPMC Hybrid System for Effective Ocular Topical Administration of Antihypertensive Agents

et al. 2020

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The increment in ocular drug bioavailability after topical administration is one of the main challenges in pharmaceutical technology. For several years, different strategies based on nanotechnology, hydrogels or implants have been evaluated. Nowadays, the tolerance of ophthalmic preparations has become a critical issue and it is essential to the use of well tolerated excipients. In the present work, we have explored the potential of gelatin nanoparticles (GNPs) loaded with timolol maleate (TM), a beta-adrenergic blocker widely used in the clinic for glaucoma treatment and a hybrid system of TM-GNPs included in a hydroxypropyl methylcellulose (HPMC) viscous solution. The TM- loaded nanoparticles (mean particle size of 193 ± 20 nm and drug loading of 0.291 ± 0.019 mg TM/mg GNPs) were well tolerated both in vitro (human corneal cells) and in vivo. The in vivo efficacy studies performed in normotensive rabbits demonstrated that these gelatin nanoparticles were able to achieve the same hypotensive effect as a marketed formulation (0.5% TM) containing a 5-fold lower concentration of the drug. When comparing commercial and TM-GNPs formulations with the same TM dose, nanoparticles generated an increased efficacy with a significant (p < 0.05) reduction of intraocular pressure (IOP) (from 21% to 30%) and an augmentation of 1.7-fold in the area under the curve (AUC)(0–12h). On the other hand, the combination of timolol-loaded nanoparticles (TM 0.1%) and the viscous polymer HPMC 0.3%, statistically improved the IOP reduction up to 30% (4.65 mmHg) accompanied by a faster time of maximum effect (tmax = 1 h). Furthermore, the hypotensive effect was extended for four additional hours, reaching a pharmacological activity that lasted 12 h after a single instillation of this combination, and leading to an AUC(0–12h) 2.5-fold higher than the one observed for the marketed formulation. According to the data presented in this work, the use of hybrid systems that combine well tolerated gelatin nanoparticles and a viscous agent could be a promising alternative in the management of high intraocular pressure in glaucoma.

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“…TIM is used as antihypertensive agent as well [32]. It was quantitatively determined by HPLC [31][32][33][34], 36] in bulk, pharmaceuticals and physiological fluids, UPLC methods [37] and an electrochmical one [38].…”

Section: Introductionmentioning

confidence: 99%

Novel Spectrophotometric Methods for Simultaneous Determination of Rosuvastatin Calcium, Timolol Maleate and Diclofenac Sodium in Pharmaceutical Ternary Mixture and Spiked Human Plasma

Hassouna

Salem

2019

JPRI

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Three sensitive, times saving, precise and accurate spectrophotometric techniques have been established and confirmed for the simultaneous determination of ternary admixture of rosuvastatin calcium (ROSCa), timolol maleate (TIM) and diclofenac sodium (DICNa). Method A, first derivative of ratio spectra spectrophotometric (1st DD) which is the most simple simplest one. Using the spectrum of an aliquot of 5 μg/mL of DICNa as a divisor, ROSCa could be determined by measuring 1DD amplitudes at 239.5 and 266.5 nm for ROSCa, while TIM could be similarly determined at 307.7 and 331 nm. DICNa could be determined at 269.7 and 292 nm when 5 μg/mL aliquot of ROSCa was used as a divisor without interference. This method showed limit of detection of 0.556, 0.567 & 0.3 µg/mL and correlation coefficients of 0.9997, 0.9998 and 0.9995 for ROSCa, DICNa and TIM respectively. Method B, depends on subjecting the ratio spectra to mean centering (MCRS). The arithmetical description of this method was explained. The method was performed for the estimation of these drugs together in one run without preseparation in authentic laboratory prepared mixtures, bulk and spiked human plasma. The wave lengths 277.9, 313.1 and 333.12 nm were used for the quantitative estimation of each of the studied drugs with good correlation coefficients (0.9998, 0.997& 0.9999 and LOD of 0.297, 0.150 & 0.24 µg/mL for the studied drugs in the previously mentioned order. Method C, successive-ratio derivative spectra method (SRDS). The mathematical description of the method was explained. By using this method, ROSCa, TIM and DICNa could be determined at 264, 339 and 300 nm, respectively. Correlation coefficients were 0.9998, 0.9999 & 0.9995 and LOD values of 0.374, 0.258 & 0.769 µg/mL in the same order. The three methods were linear in the range 5-25 µg/mL. The obtained results has been statistically compared with those obtained by the published one, showing no significant difference regarding accuracy and precision at p = 0.05. The developed methods do not need sophisticated techniques or instruments, besides being sensitive, selective and eco-friendly.

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RP-HPLC method development for the simultaneous determination of timolol maleate and human serum albumin in albumin nanoparticles

Cited by 22 publications

References 11 publications

A Novel Detection Method of Human Serum Albumin Based on the Poly(Thymine)-Templated Copper Nanoparticles

A Novel Detection Method of Human Serum Albumin Based on the Poly(Thymine)-Templated Copper Nanoparticles

Gelatin Nanoparticles-HPMC Hybrid System for Effective Ocular Topical Administration of Antihypertensive Agents

Novel Spectrophotometric Methods for Simultaneous Determination of Rosuvastatin Calcium, Timolol Maleate and Diclofenac Sodium in Pharmaceutical Ternary Mixture and Spiked Human Plasma

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