RP-HPLC Method Development and Validation for Simultaneous Estimation of Lignocaine Hydrochloride and Clotrimazole Hydrochloride in Ear Drops

gandla, kumaraswamy; Kumar, Dhiraj; Praveen, Joru; Suman, Emmadi

doi:10.5958/2231-5675.2017.00026.6

Cited by 9 publications

(2 citation statements)

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“…Lidocaine was official in British Pharmacopoeia which recommends potentiometric non-aqueous method for its analysis [4]. Literature survey reveals that numerous methods have been published for quantitative analysis of Lidocaine hydrochloride alone and in combination with other drugs such as high-performance liquid chromatography HPLC [5][6][7][8], gas chromatography [9], capillary electrophoresis [10], selective membrane electrode [11] and spectrophotometric methods [12][13][14][15]. The present work describes a new, simple spectrophotometric method for the determination of Lidocaine hydrochloride in pure form, pharmaceutical formulations and in industrial wastewater samples.…”

Section: Introductionmentioning

confidence: 99%

Beneath the Surface: A Case of Intussusception due to a Submucosal Lipoma in an Adult Patient

Alsobyani,

Akbar,

Jastaniah

et al. 2024

oajs

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A simple, rapid, accurate and sensitive spectrophotometric method for determination of Lidocaine hydrochloride has been developed. The proposed method is based on the reaction between chloride ion and mercuric thiocyanate, formation of a colored complex by the reaction between released thiocyanate and ferric ions to form red soluble product with maximum absorption at 460 nm. Beer's law is obeyed over the concentration range of 2-28µg/ml, with molar absorptivity of 0.659×104 l/mol.cm. The present method is simple because it does not need either heating or hydrolysis or solvent extraction steps. The method has been successfully applied for the determination of Lidocaine hydrochloride in pure form, pharmaceutical preparations (Gels and Ampules) and environmental wastewater sample.

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Section: Introductionmentioning

confidence: 99%

Beneath the Surface: A Case of Intussusception due to a Submucosal Lipoma in an Adult Patient

Alsobyani,

Akbar,

Jastaniah

et al. 2024

oajs

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show abstract

“…Bu yaygın kullanılan antifungal ajanların kalitatif ve kantitatif tayini için en yaygın kullanılan analitik teknik yüksek performans sıvı kromatografidir. Bu teknik içerisinde bu grubun analizi için en çok ters faz sıvı kromatografi (RPLC) metodu tercih edilmektedir [5][6][7][8][9]. RPLC metot optimizasyonunda en önemli amaç en iyi ayırma koşullarını sağlamaktır.…”

Section: Introductionunclassified

Reverse Phase Liquid Chromatography Method Optimization for Analysis of Some Azole Group Antifungal Compounds

BAŞAT DERELİ,

ÇUBUK DEMİRALAY,

KONÇE

2023

Süleyman Demirel Üniversitesi Sağlık Bilimleri Dergisi

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Bu çalışmada azol türevi antifungal ilaç etken maddeleri klotrimazol, lanokonazol ve klimbazolün ters faz sıvı kromatografi (RPLC) yöntemi kullanılarak geliştirilen metot optimizasyonunda, bileşiklerin kapasite faktörü değerleri ve analizlendikleri hareketli fazdaki metanol derişimi ilişkisi esas alınmıştır. İyonlaşan gruba sahip bu bileşiklerin optimizasyonunda hareketli faz pH değeride optimizasyonda bir diğer etkendir. İzokratik bir ayırma için kapasite faktörü değerlerinin 1-5 aralığında olması tercih edilmektedir. RPLC yöntemiyle gerçekleştirilen klotrimazol, lanokonazol ve klimbazol bileşiklerinin aynı anda tayini için yapılan optimizasyonda %50, %55 ve %60 (v/v) metanol içeren metanol-su ikili karışımları kullanılmıştır. Ayırma için çalışılan ortamlarda iki hareketli faz pH değerindeki (pH 4,0 ve 5,5) alıkonma zamanları belirlenmiştir. Hidrofobik özellikteki bileşiklerin tayini için Zorbax SB-CN (4,6x150mm) kolon tercih edilmiştir. Kolon sıcaklığı 37oC’de ve akış hızı da 1 mL/dakikada sabitlenmiştir. Optimizasyon koşullarının belirlenmesinde logk- % (v/v) metanol ilişkisi ve bileşiklerin ayrılmalarını gösteren seçicilik faktörü (α) parametresinden yararlanılmıştır.

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Retention behaviour of analytes in reversed‐phase high‐performance liquid chromatography—A review

V.¹,

Basuri²,

Sahini³

et al. 2022

Biomedical Chromatography

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The understanding of principles that drive the separation in reversed‐phase chromatography plays an important role in the prediction of the elution of solutes in RP‐HPLC. The separation in RP‐HPLC is based on the principle of adsorption and partition. In addition, the logP value, the pKa value of the drug and chromatographic parameters like mobile phase pH, buffer concentration, organic modifier and mobile phase additives also influence the retention and selectivity of the analyte. It was found that hydrophobic, electrostatic, hydrogen bonding and other specific interactions between the stationary phase and the solutes, along with the hydrophobicity of an analyte molecule (logP), modify the retention behaviour of the analytes. This article gives special attention to the influence of ionization and ion interaction on the separation of analytes. The drug molecules with different logP values containing protonated and deprotonated acids, bases and zwitterions are selected as examples and this article addresses various issues related to the method development, relationships between analyte retention and mobile phase pH and the pKa value of the analyte. The advances in this regard, with highlights on topics such as mechanisms of retention and various factors that influence the retention behaviour of analytes, are also updated with suitable examples.

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RP-HPLC Method Development and Validation for Simultaneous Estimation of Lignocaine Hydrochloride and Clotrimazole Hydrochloride in Ear Drops

Cited by 9 publications

References 0 publications

Beneath the Surface: A Case of Intussusception due to a Submucosal Lipoma in an Adult Patient

Beneath the Surface: A Case of Intussusception due to a Submucosal Lipoma in an Adult Patient

Reverse Phase Liquid Chromatography Method Optimization for Analysis of Some Azole Group Antifungal Compounds

Retention behaviour of analytes in reversed‐phase high‐performance liquid chromatography—A review

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