RP‐HPLC determination of phenylalkanoids and monoterpenoids in Rhodiola rosea and identification by LC‐ESI‐TOF

Abstract

An HPLC method permitting the simultaneous determination of fourteen analytes (phenylalkanoids and monoterpenoids) from the roots of Rhodiola rosea was developed. A separation was achieved within 35 min using C(18) column material and a water-acetonitrile mobile phase, both containing a 0.05% phosphoric acid gradient system and a temperature of 53 degrees C. The method was validated for linearity, repeatability, limits of detection and limits of quantification. The limits of detection and limits of quantification of 14 phenylalkanoids and monoterpenoids were found to be 0.20-1.0 and 0.5-3.5 microg/mL, respectively. The wavelengths used for quantification of phenylalkanoids and monoterpenoids with a diode array detector were 205, 220 and 251 nm. The method was used to analyze the roots of two species of Rhodiola and commercial extracts of R. rosea and provides preliminary evidence of phytochemical differences between North American and Eurasian populations of R. rosea. LC-mass spectrometry coupled with electrospray ionization (ESI) interface method is described for the identification of phenylalkanoids and monoterpenoids in various Rhodiola samples. This method involved the use of the [M + H](+), [M + NH(4)](+) and [M + Na](+) ions in the positive ion mode with extractive ion monitoring (EIM).

LC-MS Profile, Gastrointestinal and Gut Microbiota Stability and Antioxidant Activity of Rhodiola rosea Herb Metabolites: A Comparative Study with Subterranean Organs

“…Benzyl alcohol ( 56 ) and three of its O -glucosides, benzyl alcohol O -hexoside 53 and two benzyl alcohol O -hexoside- O -pentosides 51 and 52, were also detected as simple phenolics in the whole plant. The known benzyl alcohol O -glucoside was isolated from the roots of R. rosea [ 62 ] along with benzyl alcohol O -(6″- O -arabinopyranosyl)-glucoside [ 63 ] and benzyl alcohol O -(6″- O -arabinofuranosyl)-glucoside [ 64 ].…”

Enhancing the Accumulation of Rosavins in Rhodiola rosea L. Plants Grown In Vitro by Precursor Feeding

“…Eluents consisted of 0.1% v/v formic acid (A) and acetonitrile (B). The gradient program of Avula et al [26] was applied: 0.0 min, 5% B; 30.0 min, 19% B; 35 min, 19% B. The solvent flow rate was 1.0 mL/min, and the column temperature was set to 25 • C; 10 µL extracts were subjected to analyses on the column.…”

A supercritical fluid workflow for the quality assessment of herbal drugs and commercial preparations from Rhodiola rosea

“…Commonly applied extraction procedures for rose root samples comprise sonication and maceration using solvents such as methanol or ethanol or hydroethanolic/methanolic mixtures ranging from 38% to 75% alcohol. 16 , 17 , 18 , 19 , 20 , 21 , 22 Moreover, accelerated solvent extraction with 85% methanol and 15% water was reported. 19 …”

“… 16 , 17 , 18 , 20 , 23 However, up until now, published protocols are suffering from drawbacks, e.g. long analysis times of more than 30 minutes 16 , 19 , 21 , 22 , 23 or they are limited to the analysis of only one or few compound classes. 18 , 20 , 24 …”