Rp-HPLC Analysis of the Compounds in the Synthesis Mixtures of 2,2-Dimethyl-3-Hydroxypropionaldehyde by Two Methods Combined to One Instrument by a Column Switching Technique

Koivusalmi, Eija; Haatainen, E.; Nikkilä, Kaisa

doi:10.1081/jlc-100100500

Cited by 3 publications

(1 citation statement)

References 6 publications

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“…The most important technique used in the separation, identification, and determination of organic and inorganic compounds in biological, pharmacological, nutritional, environmental and industrial samples is high performance liquid chromatography (HPLC) [1]. A standard HPLC device consists of a mobile phase reservoir, pump, injector, column, detector, and recorder [2].…”

Section: Introductionmentioning

confidence: 99%

Applicability of Phenolic Profile Analysis Method Developed with RP-HPLC-PDA to some Bee Product

Kara

Can

Kolaylı

2022

Braz. arch. biol. technol.

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In this work, a reverse phase high-performance liquid chromatographic method (RP-HPLC) with photo diode array (PDA) at 4 different wavelengths (250, 280, 320 and 360) for the determination of some polyphenols in some bee products (honey, pollen, and propolis) is developed. The analyses were carried out on C18 column (250 mm x 4.6 mm, 5 µm; GL Sciences), and the gradient program was used with a mobile phase A reservoir with 70% acetonitrile and the B reservoir with 2% acetic acid. The HPLC method was founded that limit of detection was in the range 0.022-0.0908 µg/mL; the limit of quantification was in the range 0.074-0.3027 µg/mL, all calibration curves were linear R 2 >0.995 within the range, the recovery range was 91.43-111.37% for 10 ppm and 98.44-101.68% for 40 ppm and relative error levels 0.0330-0.0290 respectively. The developed method was applied to some bee products available on the Turkey market. The study aimed at phenolic profiles of the bee products extracts were revealed by using 25 phenolic standards. The proposed method was optimized, quickly, and simple validated by evaluating the linear range, the limits of detection and quantification, the accuracy, the precious, the repeatability, and recoveries suitable for the phenolic analysis. It is concluded that evaluated and the quantitative determination of the bee products can be made quickly and reliably with the optimized method.

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Section: Introductionmentioning

confidence: 99%

Applicability of Phenolic Profile Analysis Method Developed with RP-HPLC-PDA to some Bee Product

Kara

Can

Kolaylı

2022

Braz. arch. biol. technol.

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Determination of free formaldehyde in foundry resins as its 2,4-dinitrophenylhydrazone by liquid chromatography

Oliva-Teles¹,

Paíga²,

Delerue–Matos³

et al. 2002

Analytica Chimica Acta

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Formaldehyde is a toxic component that is present in foundry resins. Its quantification is important to the characterisation of the resin (kind and degradation) as well as for the evaluation of free contaminants present in wastes generated by the foundry industry. The complexity of the matrices considered suggests the need for separative techniques. The method developed for the identification and quantification of formaldehyde in foundry resins is based on the determination of free carbonyl compounds by derivatization with 2,4-dinitrophenylhydrazine (DNPH), being adapted to the considered matrices using liquid chromatography (LC) with UV detection. Formaldehyde determinations in several foundry resins gave precise results. Mean recovery and R.S.D. were, respectively, >95 and 5%. Analyses by the hydroxylamine reference method gave comparable results. Results showed that hydroxylamine reference method is applicable just for a specific kind of resin, while the developed method has good performance for all studied resins.

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Determination of Highly Polar Compounds in Reaction Mixtures of 2,2-Bis(hydroxymethyl)propionic Acid and 3-Hydroxy-2,2- Bis(hydroxymethyl)propionic Acid by HPLC

Koivusalmi

Tulisalo

2001

Journal of Liquid Chromatography & Related Technologies

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Rp-HPLC Analysis of the Compounds in the Synthesis Mixtures of 2,2-Dimethyl-3-Hydroxypropionaldehyde by Two Methods Combined to One Instrument by a Column Switching Technique

Cited by 3 publications

References 6 publications

Applicability of Phenolic Profile Analysis Method Developed with RP-HPLC-PDA to some Bee Product

Applicability of Phenolic Profile Analysis Method Developed with RP-HPLC-PDA to some Bee Product

Determination of free formaldehyde in foundry resins as its 2,4-dinitrophenylhydrazone by liquid chromatography

Determination of Highly Polar Compounds in Reaction Mixtures of 2,2-Bis(hydroxymethyl)propionic Acid and 3-Hydroxy-2,2- Bis(hydroxymethyl)propionic Acid by HPLC

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