RP-HPLC analysis of phenolic antioxidant compound 6-gingerol from different ginger cultivars

Pawar, Nilesh V.; Pai, Sandeep R.; Nimbalkar, Mansingraj S.; Dixit, G. B.

doi:10.1016/j.foodchem.2010.11.090

Cited by 95 publications

(87 citation statements)

References 24 publications

(21 reference statements)

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“…Our values were higher than those reported by [32], who compared TPC of 12 different ginger varieties, and found that the TPC ranging from (0.7-1.58 mg tannic acid eq./ g dry weight), but lower than those reported by ( [33]), who found that the total phenols of the alcohol extracts of ginger were 870.1 mg/g dry extract.…”

Section: Total Phenolic and Flavonoid Contentscontrasting

confidence: 88%

“…TFC ranged from (0.24 to 0.15 mg QE/g DW) (Table 2), the initial content is comparable to the results reported by [32] who obtained a TFC ranged from 0.13 to 0.38mg quercetin eq./g DW. Based on our finding, TFC was not affected by drying temperature; nevertheless, the meteorological conditions, season and post-harvest conditions have been reported as additional source of variance in the TFC [34].…”

Section: Total Phenolic and Flavonoid Contentssupporting

confidence: 85%

See 1 more Smart Citation

Impact of Infra-red drying temperature on total phenolic and flavonoid contents, on antioxidant and antibacterial activities of ginger (Zingiber officinale Roscoe).

Jihène¹

2013

IOSR-JESTFT

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Section: Total Phenolic and Flavonoid Contentscontrasting

confidence: 88%

Section: Total Phenolic and Flavonoid Contentssupporting

confidence: 85%

Impact of Infra-red drying temperature on total phenolic and flavonoid contents, on antioxidant and antibacterial activities of ginger (Zingiber officinale Roscoe).

Jihène¹

2013

IOSR-JESTFT

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“…Gingerols and shogaols give ginger its pungency and consist of a homologous series of aldols each containing a phenolic group. Its major pungent constituent, [6]-gingerol has been reported to exhibit antioxidative activity against linoleic acid autoxidation and peroxidation of phospholipid liposomes and to scavenge trichloromethylperoxyland 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals (Aeschbach et al, 1994;Sekiwa et al, 2000;Pawar et al, 2011). In addition to these antioxidative effects, our recent study (Ippoushi et al, 2003;Ippoushi et al, 2005) revealed that [6]-gingerol inhibits nitric oxide synthesis in activated J774.1 macrophages and prevents oxidation and nitration reactions induced by peroxynitrite (Radi et al, 2001), a strong reactive nitrogen species.…”

Section: Introductionmentioning

confidence: 99%

RP-HPLC analysis of phenolic antioxidant compound 6-gingerol from in vitro cultures of Zingiber officinale Roscoe

et al. 2015

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Relation between 6-gingerol content and antioxidant activity in in vitro grown cultures of ginger was studied. Reverse phase HPLC analysis revealed that rhizome derived callus culture and micropropagated plants produced lowest amount of 6-gingerol compare to conventionally grown plants. The antioxidant activity of extracts was determined using 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging assay and Ferric Reducing power assay (FRAP) and correlated with the content of total phenolics and total flavonoids in the extracts. Strong correlation was found between antioxidant activity, total phenolics and 6-gingerol content.

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“…The proposed methods such as linearity, precision, repeatability and reproducibility, accuracy, limit of detection (LOD) and limit of quantification (LOQ) was validated according to the International Conference on Harmonization (ICH) guidelines [7][8][9][10][11][12][13][14][15] .…”

Section: Validation Of the Proposed Methodsmentioning

confidence: 99%

“…Weighted amount of gingerol (10 mg) was transferred in 10 ml volumetric flask and dissolved in methanol to get 1000 µg mL -1 concentration which was used for forced degradation [7][8][9][10][11][12][13][14][15] .…”

Section: Force Degradation Studiesmentioning

confidence: 99%

Stability-Indicating Rp-HPLC Method Development for the Estimation of Gingerol

Singh¹,

Gangadharappa²,

Narke³

et al. 2017

Int. Res. J. Pharm.

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Gingerol is an analgesic, hypoglycemic, hepatoprotective, immune-stimulant, anti-inflammatory agents. In traditional medicine, gingerol has been proven plenty of pharmacological properties such as bactericidal, microbicidal, potent antioxidant, antifertility, tuberculostatic and anticancer. The key objective of this work was to develop, validate and estimate gingerol by RP-HPLC method. According to the ICH guidelines, a stabilityindicating RP-HPLC method was developed and validated. In this method, reverse phase enable Cosmosil C18 column (250 × 4.6 mm, 5μm) was used in isocratic mode. The mobile phase consisted of acetonitrile: methanol: water (52: 8: 40 v/v) delivered at a flow rate of 1.0 mL min -1 . The elute injection volume was 20 µL that was analyzed by a UV detector at 280 nm. Linearity range of the concentration of gingerol was 20 μg mL -1 -140 μg mL -1 and the correlation coefficient R 2 value was found to be 0.997 ± 0.11. Recovery was found to be in the range of 98.16 -100.07 %. The limit of detection (LOD) of gingerol was found to be 2.23 μg mL -1 and limit of quantitation (LOQ) was 5.02 μg mL -1 . Gingerol was subjected to stress conditions including acidic, alkaline, neutral, oxidation, and dry heat degradation. Gingerol was more sensitive to alkaline, dry heat and oxidative degradation and it is stable at acidic conditions. The method was validated according to ICH guidelines.

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RP-HPLC analysis of phenolic antioxidant compound 6-gingerol from different ginger cultivars

Cited by 95 publications

References 24 publications

Impact of Infra-red drying temperature on total phenolic and flavonoid contents, on antioxidant and antibacterial activities of ginger (Zingiber officinale Roscoe).

Impact of Infra-red drying temperature on total phenolic and flavonoid contents, on antioxidant and antibacterial activities of ginger (Zingiber officinale Roscoe).

RP-HPLC analysis of phenolic antioxidant compound 6-gingerol from in vitro cultures of Zingiber officinale Roscoe

Stability-Indicating Rp-HPLC Method Development for the Estimation of Gingerol

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