RP-HPLC analysis of diterpene lactones in leaves and stem of different species of <i>Andrographis</i>

Dalawai, Dayanand; Aware, Chetan; Jadhav, Jyoti P.; Murthy, Hosakatte Niranjana

doi:10.1080/14786419.2019.1662004

Cited by 17 publications

(12 citation statements)

References 10 publications

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“…Although Andrographis paniculata is the primary source for these compounds, increasing demand for this species necessitates searching for alternate sources. Another species of Andrographis , A. alata , is rich in neoandrographolide and shows antipyretic, anti-inflammatory, and antiviral properties ( Balu et al, 1993 , Balu and Alagesaboopathi, 1993 , Dalawai et al, 2021 ). However, compared to A. paniculata , research on A. alata is extremely limited.…”

Section: Discussionmentioning

confidence: 99%

“…Ethanol extract from A. alata was reported to have antipyretic and anti-inflammatory activities in male albino rats ( Balu et al, 1993 , Balu and Alagesaboopathi, 1993 ). A. alata leaves are an excellent source of neoandrographolide with 33.21 mg/g dry weight ( Dalawai et al, 2021 ). In vitro regenerated A. alata plants contained 32.21 mg/g dry weight neoandrographolide ( Kadapatti and Murthy, 2021 ).…”

Section: Introductionmentioning

confidence: 99%

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Whole transcriptome analysis identifies full-length genes for neoandrographolide biosynthesis from Andrographis alata, an alternate source for antiviral compounds

Tanuja

Parani²

2023

Gene

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Whole transcriptome analysis identifies full-length genes for neoandrographolide biosynthesis from Andrographis alata, an alternate source for antiviral compounds

Tanuja

Parani²

2023

Gene

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“…Therefore, plant regeneration protocols have been developed for A. paniculata [10], A. alata [11], A. macrobotrys [12], A. echioides [13], and A. lineata [14]. In addition to the facts mentioned above, conventional propagation of Andrographis producta through seeds is hampered by poor seed germination and short seed viability [15,16], and there are no regeneration protocols for the micropropagation of Andrographis producta. Given the above, the In vitro propagation method was adopted for the large-scale production of Andrographis producta plants.…”

Section: Introductionmentioning

confidence: 99%

Micropropagation of Andrographis producta through axillary and adventitious shoot regeneration

Kadapatti

Murthy

2022

Journal of Genetic Engineering and Biotechnology

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Background Andrographis producta (C. B. Clarke) Gamble is a valuable medicinal plant that yields several therapeutic compounds. In addition, this species is endemic to the Western Ghats regions of South India. Natural populations of Andrographis producta have dwindled due to the overexploitation of this species. The objective of the present study was to develop a reliable In vitro propagation protocol for this plant species. Results In vitro plant regeneration protocol has been developed in Andrographis producta using nodal and shoot tip explants. The highest axillary shoots (14.60) were regenerated from nodal explants on MS medium amended with 10 μM 2-iP. Similarly, on MS media amended with 5 μM BAP, 17.50 shoots were regenerated from shoot tip explants. Optimal of 27.66 adventitious shoots were regenerated from the cut end of shoot tip explants on MS medium amended with 10 μM 2-iP. Medium amended with 10 μM 2-iP was optimum for regeneration of multiple axillary shoots from nodal explants and for the adventitious shoots regeneration from shoot tip explants. Shoot tips were ideal explants for the micropropagation of A. producta. Qarter sthrength MS media supplemented with 10 μM IBA has resulted in maximum rooting of the shoots. Conclusions A reliable In vitro micropropagation method was developed in Andrographis producta through direct organogenesis, and this method is helpful for the multiplication, conservation, and utilization of this plant.

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“…Clarke is an underexplored species, though it has a notable quantity of andrographolide. 9 Given the above, the present study was carried out with the objective isolation, characterization of andrographolide from Andrographis lineata Wall. ex Nees var.…”

Section: Introductionmentioning

confidence: 99%

Isolation of Andrographolide from Andrographis lineata Wall. ex Nees var. lawii C.B. Clarke and its Anticancer Activity against Human Ovarian Teratocarcinoma

Bhat¹,

Murthy²

2021

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Introduction: Andrographolide is a well-known anticancer phytochemical often isolated from Andrographis paniculata (Burm. f.) Nees. (Acanthaceae). Though Andrographis lineata Wall. ex Nees var. lawii C.B. Clarke (ALw) which also belongs to the same family has an adequate amount of andrographolide; remained untouched for isolation of andrographolide and anticancer studies. Therefore, this study was targeted to isolate the andrographolide from the leaves of ALw and to assess its role inthe induction of apoptosis against the human ovarian teratocarcinoma (PA-1) cell line. Methods: Column chromatography, thin-layer chromatography (TLC), preparative TLC were used for the isolation and purification while melting point, ultraviolet (UV)-visible spectroscopy, Fourier transform infrared (FTIR), proton nuclear magnetic resonance ( 1 H NMR), carbon-13 (C13) nuclear magnetic resonance ( 13 C NMR) analysis were carried out for characterization of the compound. 3-(4,5-dimethylthiaxo-2yl) 2, 5-diphenyl tetrazolium bromide (MTT) assay was carried out for cytotoxicity test and further Annexin-V staining, caspase 3 activity, B-cell lymphoma-2 (Bcl-2) activity, cell cycle analysis, and DNA damage study by terminal deoxynucleotidyl transferase (dUTP) nick end labeling (TUNEL) assays were carried out for apoptosis study. Results: Andrographolide was isolated from the methanolic extract of leaves of ALw which had a melting point of 230 ºC, λ max at 223 nm. FTIR results proved the presence of hydroxyl group, alkanes, carbon-carbon double bond, and a characteristic gamma lactone carbonyl. NMR data confirmed the 20 carbon structure. In the MTT assay cytotoxicity against PA-1 was at 3.7 µg/ml with other apoptotic assays supporting the induction of apoptosis by the compound at that concentration. Conclusion: ALw is proved to be an alternate source of andrographolide with potential abilities to induce apoptosis in ovarian cancer cells.

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RP-HPLC analysis of diterpene lactones in leaves and stem of different species of Andrographis

Cited by 17 publications

References 10 publications

Whole transcriptome analysis identifies full-length genes for neoandrographolide biosynthesis from Andrographis alata, an alternate source for antiviral compounds

Whole transcriptome analysis identifies full-length genes for neoandrographolide biosynthesis from Andrographis alata, an alternate source for antiviral compounds

Micropropagation of Andrographis producta through axillary and adventitious shoot regeneration

Isolation of Andrographolide from Andrographis lineata Wall. ex Nees var. lawii C.B. Clarke and its Anticancer Activity against Human Ovarian Teratocarcinoma

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