Royal jelly affects collagen crosslinking in bone of ovariectomized rats

Kaku, Masaru; Rocabado, Juan Marcelo Rosales; Kitami, Megumi; Ida, Takako; Uoshima, Katsumi

doi:10.1016/j.jff.2014.01.019

Cited by 13 publications

(14 citation statements)

References 35 publications

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“…Rats were randomly divided into two groups: six rats in the control group and seven rats in the ovariectomy-induced osteoporosis group (OVX group). Bilateral ovariectomy [ 21 , 22 ] was performed in the OVX group at 10 weeks of age according to the protocol and the ethical guidelines of Niigata University (36-9). The animals were provided with commercially available pellet food and water ad libitum and kept under controlled light conditions of 12/12-h light/dark cycles throughout the experiment.…”

Section: Methodsmentioning

confidence: 99%

A multi-factorial analysis of bone morphology and fracture strength of rat femur in response to ovariectomy

et al. 2018

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BackgroundPostmenopausal osteoporosis develops due to a deficiency of estrogen that causes a decrease in bone mass and changes in the macro- and micro-architectural structure of the bone, leading to the loss of mechanical strength and an increased risk of fracture. Although the assessment of bone mineral density (BMD) has been widely used as a gold standard for diagnostic screening of bone fracture risks, it accounts for only a part of the variation in bone fragility; thus, it is necessary to consider other determinants of bone strength. Therefore, we aimed to comprehensively evaluate the architectural changes of the bone that influence bone fracture strength, together with the different sensitivities of cortical and trabecular bone in response to ovariectomy (OVX).MethodsBone morphology parameters were separately analyzed both in cortical and in trabecular bones, at distal-metaphysis, and mid-diaphysis of OVX rat femurs. Three-point bending test was performed at mid-diaphysis of the femurs. Correlation of OVX-induced changes of morphological parameters with breaking force was analyzed using Pearson’s correlation coefficient.ResultsOVX resulted in a decline in the bone volume of distal-metaphysis trabecular bone, but an increase in distal-metaphysis and mid-diaphysis cortical bone volume. Tissue mineral density (TMD) remained unchanged in both the trabecular and cortical bone of the distal metaphysis but decreased in cortical bone of the mid-diaphysis. The OVX significantly increased the breaking force at mid-diaphysis of the femurs.ConclusionsOVX decreased the trabecular bone volume of the distal-metaphysis and increased the cortical bone volume of the distal-metaphysis and mid-diaphysis. Despite the reduction in TMD and increased cortical porosity, bone fracture strength increased in the mid-diaphysis after OVX. These results indicate that analyzing a single factor, i.e., BMD, is not sufficient to predict the absolute fracture risk of the bone, as OVX-induced bone response vary, depending on the bone type and location. Our results strongly support the necessity of analyzing bone micro-architecture and site specificity to clarify the true etiology of osteoporosis in a clinical setting.

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Section: Methodsmentioning

confidence: 99%

A multi-factorial analysis of bone morphology and fracture strength of rat femur in response to ovariectomy

et al. 2018

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“…Actually, bone fragility is strongly related with collagen metabolism in post-menopausal women [ 18 ]. In contrast, it is reported that RJ could contribute bone quality due to modulating the posttranslational modification of type I collagen [ 11 ]. Then, RJP might involve bone strength via other factor of bone volume such as collagen metabolism, but that possibility needs further consideration.…”

Section: Discussionmentioning

confidence: 99%

“…In contrast, royal jelly protein (RJP) is a latest source of fresh royal jelly 10 . It may have possibilities such as osteoblast activation 11 and osteoclast differentiation through attenuating the induction of nuclear factor of activated T cells (NFAT) c1 (a key transcription factor for osteoclast differentiation) 9 like a RJ ingestion. Thus, RJP may also have the beneficial effects of bone Satoshi Hattori 1, § / Naomi Omi 1, §, * health, whereas the effectiveness is not well known.…”

Section: Introductionmentioning

confidence: 99%

The effects of royal jelly protein on bone mineral density and strength in ovariectomized female rats

Hattori

Omi

2021

Phys Act Nutr

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[Purpose] Sex hormones deficiency leads to dramatically bone loss in particular postmenopausal women. Royal jelly has anti-osteoporosis effect due to maintain bone volume in that condition. We hypothesized that royal jelly protein (RJP, a latent residue after extracting royal jelly) also prevents bone deficient in ovariectomized (OVX) female rats, the animal model of postmenopausal women. [Methods] Female Sprague-Dawley rats (n = 30, 6 weeks age old) were sham operated (Sham; sham operated group, n = 7), OVX control group (OC, n = 7), OVX with low RJP intake group (ORL, n = 8), and OVX with high RJP intake group (ORH, n = 8) during 8 weeks experimental periods. In the end point of this experiment, the bone samples (lumbar spine, tibia, and femur) were surgically removed under anesthesia. These bone samples were evaluated bone mineral density (BMD) and bone strength.[Results] BMD of lumbar spine in RJP intake groups (ORL, ORH) were higher than that in OC group (p < 0.05 and p < 0.01) in RJP intake volume dependent manner. BMD of tibial proximal metaphysis and diaphysis in RJP intake groups were also higher than these in OC group (p < 0.01 and p < 0.01 / p < 0.05 and p < 0.001). In addition, breaking force of femur in RJP intake groups were significantly increase compared with that in OC group (p < 0.001 respectively). [Conclusion] These findings indicate that RJP contribute to prevent sex hormone related bone abnormality

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“…RJ presented an improvement in the bone quality of ovariectomized rats by a reduction in collagen crosslink (pyridinoline and deoxypyridinoline) and stimulation of the expression of collagen-modifying enzymes. These effects produced posttranslational modifications in type I collagen [ 166 ]. To find the compounds involved and the mechanism of this activity, a study in vitro with 10-hydroxy-2-decenoic acid (10H2DA) was recently performed.…”

Section: Biological and Health Promoting Propertiesmentioning

confidence: 99%

Health Promoting Properties of Bee Royal Jelly: Food of the Queens

et al. 2021

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Royal jelly (RJ) demand is growing every year and so is the market for functional foods in general. RJ is formed by different substances, mainly carbohydrates, proteins, and lipids, but also vitamins, minerals, and phenolic or volatile compounds in lower proportion. Major royal jelly proteins (MRJP) are, together with 10-hydroxy-2-decenoic acid (10-HDA), key substances of RJ due to their different biological properties. In particular, 10-HDA is a unique substance in this product. RJ has been historically employed as health enhancer and is still very relevant in China due to the traditional medicine and the apitherapy. Nowadays, it is mainly consumed as a functional food or is found in supplements and other formulations for its health-beneficial properties. Within these properites, anti-lipidemic, antioxidant, antiproliferative, antimicrobial, neuroprotective, anti-inflammatory, immunomodulatory, antiaging, and estrogenic activities have been reported for RJ or its specific components. This manuscript is aimed at reviewing the current knowledge on RJ components, their assessment in terms of authenticity, their biological activities, and related health applications.

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Royal jelly affects collagen crosslinking in bone of ovariectomized rats

Cited by 13 publications

References 35 publications

A multi-factorial analysis of bone morphology and fracture strength of rat femur in response to ovariectomy

A multi-factorial analysis of bone morphology and fracture strength of rat femur in response to ovariectomy

The effects of royal jelly protein on bone mineral density and strength in ovariectomized female rats

Health Promoting Properties of Bee Royal Jelly: Food of the Queens

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