Roxithromycin promotes lymphocyte apoptosis in Dermatophagoides-sensitive asthma patients

Ogawa, N; Sugawara, Yoko; Fujiwara, Yoshitaka; Noma, Takeshi

doi:10.1016/s0014-2999(03)01998-8

Cited by 21 publications

(19 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Identifying mechanisms which modulate the populations of memory T-cell subsets has been suggested as a therapeutic strategy to reduce the inflammatory response in allergic asthma [27,28]. The local suppression or removal of memory lymphocytes may reduce the release of pro-inflammatory cytokines that could contribute to airways hyperresponsiveness, inflammation and remodelling in asthma.…”

Section: Discussionmentioning

confidence: 99%

Reduced apoptosis of memory T-cells in the inner airway wall of mild and severe asthma

Lamb

James²,

Carroll³

et al. 2005

Eur Respir J

View full text Add to dashboard Cite

Effector memory T-cells (CD45RO+) may provide pro-inflammatory signals that contribute to the persistent airway inflammation that is characteristic of asthma, and reduced apoptosis of these cells may prolong their effects.The present authors compared apoptosis of CD45RO+ T-cells in the inner airway wall in nonfatal asthma (n57), fatal asthma (n57) and control (n58) cases. Apoptotic cells were identified using both the terminal deoxynucleotidyl transferase dNTP nick end-labelling (TUNEL) technique and cell morphology.The percentage of CD45RO+ T-cells that were apoptotic was significantly greater in control cases compared with nonfatal and fatal cases of asthma, respectively, in small (42¡19, 16¡9, 7¡6%), medium (40¡12, 15¡11, 12¡8%) and large airways (42¡15, 23¡18, 18¡12%). The reduction in the percentage of apoptotic CD45RO+ cells in the cases of asthma was observed in both blood vessels and the interstitium in large airways.In conclusion, these data suggest that reduced apoptosis may prolong the active life of effector memory T-cells in the airways. It is possible that survival signals may be received before cells migrate into the interstitium of the inner airway wall.

show abstract

Section: Discussionmentioning

confidence: 99%

Reduced apoptosis of memory T-cells in the inner airway wall of mild and severe asthma

Lamb

James²,

Carroll³

et al. 2005

Eur Respir J

View full text Add to dashboard Cite

show abstract

“…Several studies have evaluated different macrolides administered either short-term or long-term, with or without corticosteroids and montelukast, in terms of their efficacy in reducing exacerbation rates and stabilising/increasing respiratory capacity (Table 2) [44, 136,140,[155][156][157][158][159][160][161][162][163][164][165][166][167][168][169][170]. Kutlin et al [166] assessed macrolide treatment with levofloxacin and obtained positive results.…”

Section: Macrolides and Asthmamentioning

confidence: 99%

“…Kutlin et al [166] assessed macrolide treatment with levofloxacin and obtained positive results. Ogawa et al [159] observed that roxithromycin promoted lymphocyte apoptosis in Dermatophagoides-sensitive asthma patients. Low concentrations of roxithromycin (1-500 ng/ml) augmented the early, but not the late, phase of apoptosis in Dermatophagoides farinae-stimulated peripheral blood mononuclear (PBM) cells.…”

Section: Macrolides and Asthmamentioning

confidence: 99%

Macrolides: from in vitro anti-inflammatory and immunomodulatory properties to clinical practice in respiratory diseases

Zarogoulidis

Παπάνας

Kioumis

et al. 2011

Eur J Clin Pharmacol

255

196

View full text Add to dashboard Cite

show abstract

“…3,4,28) Furthermore, RXM is used in clinical study and the treatment of chronic inflammatory diseases such as diffuse panbronchiolitis and dermatophagoides-sensitive asthma. 29,30) Although these reports suggest that macrolides have molecular targets in eukaryotes, the molecular mechanisms by which macrolides affect eukaryotes remain poorly understood. In this study, we aimed to determine the alternative targets of RXM in eukaryotes and to establish a novel approach to identify new targets for drugs using fungi.…”

Section: Discussionmentioning

confidence: 99%

“…[40][41][42][43][44] RXM was reported to inhibit proliferation in and to promote the apoptosis of human lymphocyte, and to have an anti-tumor effect. 5,30,[45][46][47] This suggests that CDC27 is an important target of RXM in eukaryotes.…”

Section: Discussionmentioning

confidence: 99%

A Eukaryotic Molecular Target Candidate of Roxithromycin: Fungal Differentiation as a Sensitive Drug Target Analysis System

Ishii

Kumasaka

Nagashima

et al. 2013

Bioscience, Biotechnology, and Biochemistry

View full text Add to dashboard Cite

Roxithromycin (RXM), active against prokaryotes, has beneficial side effects such as anti-cancer activities on mammalian cells, but the mechanisms underlying these effects remain unclear. We found that RXM inhibited the cellular differentiation of the rice blast fungus Magnaporthe oryzae. Hence, we screened the targets of RXM by the T7 phage display method with fungal genomic DNA, and identified MoCDC27 (M. oryzae Cell Division Cycle 27) as a candidate. We generated mocdc27 knockdown mutants that the appressoria formation was less affected by RXM. A complemented mutant restored sensitivity against RXM to the level of the wild type. These results suggest that MoCDC27 was involved in the inhibition of appressorium formation by RXM, and that the complex of RXM-MoCDC27 affected another molecule involved in appressorium formation. The T7 phage display method with fungal genomic DNA can be a useful tool in the quest for drug target.

show abstract

Roxithromycin promotes lymphocyte apoptosis in Dermatophagoides-sensitive asthma patients

Cited by 21 publications

References 34 publications

Reduced apoptosis of memory T-cells in the inner airway wall of mild and severe asthma

Reduced apoptosis of memory T-cells in the inner airway wall of mild and severe asthma

Macrolides: from in vitro anti-inflammatory and immunomodulatory properties to clinical practice in respiratory diseases

A Eukaryotic Molecular Target Candidate of Roxithromycin: Fungal Differentiation as a Sensitive Drug Target Analysis System

Contact Info

Product

Resources

About