Routine use of μ‐antibody‐capture elisa for the serological diagnosis of coxsackie b virus infections

McCartney, R.A.; Banatvala, J. E.; Bell, Eleanor J.

doi:10.1002/jmv.1890190302

Cited by 82 publications

(20 citation statements)

References 4 publications

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“…This tech nique enabled us to identify specific viral antigens present in circulating complexes, and thereby to identify the infectious cause. The association of the enteroviruses with postviral fatigue syndrome has been de scribed [Behan et al" 1985: McCartney et al, 1986, Figure 2 shows that about 50% of the patients with this syndrome have IgM com plexes which react with the peroxidase-labelled 5-D8/I antibody strongly, indicating that the antigen in the complexes has speci ficity for the enterovirus group-reactive anti body.…”

Section: Discussionmentioning

confidence: 99%

“…Complexes Sera were obtained from 87 patients with chronic postviral fatigue syndrome, a condition in which an enteroviral etiology was suspected [Behan et al, 1985;McCartney et al, 1986]. Another group of sera was obtained from 32 patients with other diseases asso ciated with the formation of immune complexes [e.g., subacute bacterial endocarditis, systemic lupus erithematosus, rheumatoid arthritis etc.].…”

Section: Detection O F Antigens In Circulating Immunementioning

confidence: 99%

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Clinical and Research Application of an Enterovirus Group-Reactive Monoclonal Antibody

Yousef¹,

Mann

Brown

et al. 1987

Intervirology

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Diagnostic methods employed in enterovirus laboratories are generally laborious, slow and expensive. This is largely because type-specific neutralization tests still play the major role in identification and diagnostic serology. In the companion paper we describe the derivation of monoclonal antibodies against epitopes of the VPI peptide which are shared by all of the enteroviruses tested to date, with the exception of hepatitis A virus. This study describes the application of one of these monoclonal antibodies in several research and diagnostic procedures, illustrating a special utility in a wide variety of assay systems. This monoclonal antibody has proved particularly useful in the detection of enterovirus antigens in circulating immune complexes, and in identifying field isolates of this group of viruses. Immunohistochemistry, previously almost impossible in enterovirus diagnosis and research due to the large number of serotypes, is now shown to be practical and informative when this monoclonal antibody is used.

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Section: Discussionmentioning

confidence: 99%

Section: Detection O F Antigens In Circulating Immunementioning

confidence: 99%

Clinical and Research Application of an Enterovirus Group-Reactive Monoclonal Antibody

Yousef¹,

Mann

Brown

et al. 1987

Intervirology

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“…To detect IgM antibodies, a sandwich technique is used, with anti-antibodies on the plate to capture IgM from the test serum, followed by addition of EV antigen. The antigen may be labeled (32,43) or there may be detection reagents added, such as labeled mAbs (3) or mouse anti-CVB followed by labeled anti-mouse (42). An indirect ELISA for IgG detection, where the antigen is bound directly to the plate, is more sensitive than sandwich IgG ELISA (32) ( Table 2).…”

Section: Immunoassaysfor Detection Of Enteroviral Antibodiesmentioning

confidence: 99%

The Role of Enteroviral Infections in the Development of IDDM: Limitations of Current Approaches

et al. 1997

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Enteroviruses have been examined for their possible role in the etiology of IDDM for nearly 40 years, yet the evidence remains inconclusive. The mechanism of acute cytolytic infection of beta-cells, proposed by earlier studies, appears to be incompatible with the long preclinical period of autoimmunity preceding IDDM. Advances in molecular biology have improved our understanding of enteroviral biology and of potential alternative pathogenic mechanisms through which enteroviruses may cause diabetes. The focus of future human studies will likely shift from people with IDDM to those with prediabetic autoimmunity to determine whether acute enteroviral infections can promote progression from autoimmunity to overt diabetes. We propose that such studies use assays to detect enteroviral RNA, in addition to IgM serology. RNA assays can overcome sensitivity and type-specificity limitations of IgM assays as well as identify diabetogenic strains of enteroviruses, if such exist. Evaluation of the role of enteroviruses in triggering beta-cell autoimmunity in humans will require large prospective studies of young children. The Diabetes Autoimmunity Study in the Young--one of very few such studies currently underway--is focusing on potential interactions between HLA class II genes and enteroviral infections. Future studies will likely examine interactions between viral infections and non-HLA IDDM candidate genes, including those that may determine beta-cell tropism of candidate viruses.

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“…The detection of specific IgM has been found to be useful in the early diagnosis of enteroviral infections [McCartney et al, 1986;Magnius et al, 1988;Frisk et al, 1989;Muir and Banatvala, 1990;Blomberg and Pipkorn, 1991;Buxbaum et al, 2001;Craig et al, 2003;Imagawa et al, 2005]. A novel technique for EV IgM detection, quantitative PCR-enhanced immunoassay (QPIA) was recently introduced [Elfaitouri et al, 2005].…”

Section: Introductionmentioning

confidence: 98%

Recent enterovirus infection in type 1 diabetes: Evidence with a novel IgM method

Elfaitouri¹,

Berg

Frisk

et al. 2007

Journal of Medical Virology

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Enterovirus (EV) infection has been associated with Type 1 (T1D) diabetes and on a few occasions virus could be isolated at onset of the disease. Using two such isolates as antigens in a quantitative PCR enhanced immunoassay (T1D-EV-QPIA) we have measured IgM antibodies against such potentially diabetogenic viruses in serum from 33 newly diagnosed T1D children, 24 siblings, and 27 healthy children. Sera were also analysed with regard to autoantibodies against GAD65, the cytokine TNF-alpha and the chemokine IP-10. EV-RNA detection was performed on peripheral blood mononuclear cells (PBMC). IgM antibodies against this "new" EV antigen were more frequent in serum from T1D children than in serum from siblings and/or controls (P < 0.001). EV-RNA was detected more frequently in PBMC from T1D children than in healthy control children (P < 0.001) and also compared to the siblings (P < 0.003). The cytokine TNF-alpha was less frequently detected in serum from the T1D children compared with serum from siblings and/controls (P < 0.001). A positive correlation was found between the results obtained with the T1D-EV-QPIA and the EV-PCR (P < 0.001). These findings are in line with earlier findings of an increased frequency of enteroviral infections in newly diagnosed T1D patients. In addition, we found that T1D children at onset of the disease had lower frequencies of the chemokine TNF-alpha in their serum than age- and sex-matched controls had, suggesting an impaired immune response.

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Routine use of μ‐antibody‐capture elisa for the serological diagnosis of coxsackie b virus infections

Cited by 82 publications

References 4 publications

Clinical and Research Application of an Enterovirus Group-Reactive Monoclonal Antibody

Clinical and Research Application of an Enterovirus Group-Reactive Monoclonal Antibody

The Role of Enteroviral Infections in the Development of IDDM: Limitations of Current Approaches

Recent enterovirus infection in type 1 diabetes: Evidence with a novel IgM method

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