Routine use of hair root or buccal swab specimens for PCR analysis: Advantages over using blood

Thomson, Darelle; Brown, Nigel N.; Clague, Alan

doi:10.1016/0009-8981(92)90116-8

Cited by 50 publications

(27 citation statements)

References 8 publications

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“…The intrinsic low (1) DNA extraction on an old or degraded sample yields a low number of template molecules, one of which has undergone a post mortem damage associate cytosine (C) to thymine (T) deamination. (2) Due to the total low number of template molecules in the extract, a resulting PCR from an aliquot starts from only two amplifiable molecules, one of which is the damaged sequence. (3) All resultant amplicons derive from one of the two founder templates.…”

Section: Introductionmentioning

confidence: 99%

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Histological correlates of post mortem mitochondrial DNA damage in degraded hair

Gilbert

Janaway

Tobin

et al. 2006

Forensic Science International

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Section: Introductionmentioning

confidence: 99%

“…Hair has been used as a mitochondrial DNA (mtDNA) source for a range of genetic analyses, including forensic casework [1], medical screening [2], population genetic investigations [3] and recently, ancient DNA extractions [4,5]. On average, an individual sheds 100 hairs each day, making hair important forensic trace evidence [6].…”

Section: Introductionmentioning

confidence: 99%

Histological correlates of post mortem mitochondrial DNA damage in degraded hair

Gilbert

Janaway

Tobin

et al. 2006

Forensic Science International

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“…Blood is currently the most commonly used source of DNA for genetic testing. The disadvantages of using blood include invasive collection, need for a trained phlebotomist, special storage, and time consuming DNA extraction [8].…”

Section: Resultsmentioning

confidence: 99%

An Efficient Procedure for DNA Isolation and Profiling of the Hyper Variable MtDNA Sequences

Akbar¹,

Ahmad²,

Nadeem³

et al. 2015

JLS

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Present study describes the results of an efficient protocol for the isolation of good quality DNA from human saliva. The protocol includes collection of saliva in sterile specimen tubes, followed by the cell lysis. After formation of cell lysate, proteins wereextracted by phenol chloroform treatment for purification of DNA. The purified DNA was precipitated by adding equal volume of isopropanol to the treated supernatant. After isolation DNA pellet was washed with 70% ethanol, air-dried and was suspended in 30 µL of double distilled water. Best quality of DNA was extracted from the saliva samples and the PCR product was amplified for hyper-variable regions (HV1& HV2) of the mitochondrial DNA. The genes were cleaned with GeneAll gel elution kit (Gel SV) (Cat. No. 102-10) and sequenced accordingly. The DNA isolation protocol presented here is recommended for the isolation, best quality and yield of DNA from the human saliva.

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“…DNA was extracted from routine blood samples and/or opportunistically obtained hair follicle samples (LAHIRI & NURNBERGER, 1991;THOMPSON et al, 1992) collected from chimpanzees held in colonies throughout the Australasian region. Familial relationships between individuals and the numbers assigned to animals are taken from the regional studbook (CASLICK, 1996), compiled using SPARKS (ScOBIE, 1994).…”

Section: Sample Collection and Dna Extractionmentioning

confidence: 99%

Captive breeding, paternity determination, and genetic variation in chimpanzees (Pan troglodytes) in the Australasian region

1997

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ABSTRACT. DNA "fingerprinting" using polymorphic (CA)-repeat microsatellite markers was used to quantify the level of genetic variation present in chimpanzees (Pan troglodytes) in the Australasian region. These markers were also used to determine the paternity of chimpanzees born at Taronga Zoo over a 20-year period. The results suggested that the dominant male in the colony was responsible for siring most, but not all, of the offspring. Where the dominant male was excluded from paternity, the sire was identifiable if all candidate males were available for typing. This enabled us to prove the captive origin of offspring born in the colony during this period. Thus, microsatellite analysis was a useful tool for assignment of familial relationships and improving genetic management of breeding colonies.

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Routine use of hair root or buccal swab specimens for PCR analysis: Advantages over using blood

Cited by 50 publications

References 8 publications

Histological correlates of post mortem mitochondrial DNA damage in degraded hair

Histological correlates of post mortem mitochondrial DNA damage in degraded hair

An Efficient Procedure for DNA Isolation and Profiling of the Hyper Variable MtDNA Sequences

Captive breeding, paternity determination, and genetic variation in chimpanzees (Pan troglodytes) in the Australasian region

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