Routine overnight starvation and immunocytochemistry of hepatocyte albumin content

Av, LeBouton

doi:10.1007/bf00210896

Cited by 16 publications

(7 citation statements)

References 12 publications

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“…In contrast, hepatic swelling was observed in normal control mice. We assume that this swelling was due to overnight starvation, which is agreement with previous study (LeBouton, 1982).…”

Section: Discussionsupporting

confidence: 69%

Antroquinonol from ethanolic extract of mycelium of Antrodia cinnamomea protects hepatic cells from ethanol-induced oxidative stress through Nrf-2 activation

Kumar

Chu

Hsieh

et al. 2011

Journal of Ethnopharmacology

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“…In contrast, hepatic swelling was observed in normal control mice. We assume that this swelling was due to overnight starvation, which is agreement with previous study (LeBouton, 1982).…”

Section: Discussionsupporting

confidence: 69%

Antroquinonol from ethanolic extract of mycelium of Antrodia cinnamomea protects hepatic cells from ethanol-induced oxidative stress through Nrf-2 activation

Kumar

Chu

Hsieh

et al. 2011

Journal of Ethnopharmacology

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“…Plasma protein synthesis and secretion appear to be catalyzed normally by all hepa tocytes; a zonal heterogeneity in protein syn thesis may exist under certain conditions in some cases; e.g., after an overnight fast peri venous cells were seen to be more active in albumin synthesis [101][102][103].…”

Section: Milmentioning

confidence: 99%

Functional Heterogeneity of Periportal and Perivenous Hepatocytes

Jungermann¹

1986

Enzyme

131

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Periportal and perivenous hepatocytes differ in their content of many key enzymes and subcellular structures. The cells also receive different regulatory signals due to the gradients established during liver passage of oxygen, substrates and hormones. The signal heterogeneity is important not only for short-term regulation of metabolism but also for long-term control, i.e. the induction of liver cell heterogeneity. The zonal heterogeneity changes upon longer lasting physiological and pathological alterations of the metabolic situation such as starvation, diabetes or regeneration after partial hepatectomy; it develops only gradually during the first weeks of postnatal life. The model of‘metabolic zonation’ proposes a functional specialization for the two zones: (1) in the periportal zone oxidative energy metabolism with ß-oxidation and amino acid metabolism, ureagenesis, gluconeogenesis, cholesterol synthesis, bile formation and oxidation protection are the predominant activities, and (2) in the perivenous zone glycolysis, liponeogenesis, ketogenesis, glutamine formation and biotransformation are the prevalent processes.

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“…The volume of the hepatocytes and protein content in them decrease during fasting too (31)(32)(33). Administration of glucose to fasted rats resulted in an increase in DWH as early as 10 min after the start of refeeding in both animal groups.…”

Section: Discussionmentioning

confidence: 99%

Glycogen content in hepatocytes is related with their size in normal rat liver but not in cirrhotic one

et al. 2016

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Hepatocytes differ from one another by the degree of the ploidy, size, position in the liver lobule, and level of the DNA-synthetic processes. It is believed, that the cell size exerts substantial influence on the metabolism of the hepatocytes and the glycogen content in them. The aim of the present study was to test this hypothesis. Dry weight of hepatocytes, their ploidy and glycogen content were determined in the normal and the cirrhotic rat liver. Liver cirrhosis in rats was produced by chronic inhalation of CCl 4 vapours in the course of 6 months. A combined cytophotometric method was used. Dry weight of the cell, its glycogen and DNA content were successively measured on a mapped preparation. Hepatocytes of each ploidy class in the normal and the cirrhotic rat liver accumulated glycogen at the same rate. In the normal liver, there was a distinct correlation between the size of hepatocytes and glycogen content in them. This correlation was observed in each ploidy class, and was especially pronounced in the class of mononucleate tetraploid hepatocytes. In the cirrhotic liver, there was no correlation between the size of the cells and their glycogen content. The impairment of liver lobular structure probably explains the observed lack of correlation between hepatocyte size and their glycogen content in the cirrhotic liver. Key terms hepatocytes; glycogen; dry weight of hepatocytes; ploidy; liver cirrhosis GLUCOSTATIC activity is a major function of the liver. Hepatocytes contribute to the sustenance of a constant glucose level in the blood by synthesizing glycogen from glucose after a meal and breaking it down when necessary. Glycogenesis and glycogenolysis, ensured by several enzymes, are regulated by hormonal, nervous, substrate and other mechanisms (1,2) as well as tissue and cell factors (3,4).The population of hepatocytes is not homogeneous. These cells differ not only in their specific functional activity but also in ploidy level, size, position in the liver lobule, level of proliferative activity etc. (5-7). Glycogen content and enzymatic activity of hepatocytes are known to be determined by their location in the liver lobule, the ploidy level and the cell cycle phase (6,(8)(9)(10)(11). However, we know almost nothing about the relations between the functional activity of hepatocytes, including glycogen content and accumulation rate, and their size.The size of hepatocytes may considerably vary. Even hepatocytes of the same ploidy class may differ by several times as to volume and dry weight (12,13). The reasons of this variability are not quite clear. At the same time, the size of hepatocytes is crucial for their metabolism.It has been suggested that there is a certain relation between the size of hepatocytes and the glycogen content in them: larger cells are richer in glycogen (14). The aim of the present study was to test this hypothesis. The aim of the present study was

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Routine overnight starvation and immunocytochemistry of hepatocyte albumin content

Cited by 16 publications

References 12 publications

Antroquinonol from ethanolic extract of mycelium of Antrodia cinnamomea protects hepatic cells from ethanol-induced oxidative stress through Nrf-2 activation

Antroquinonol from ethanolic extract of mycelium of Antrodia cinnamomea protects hepatic cells from ethanol-induced oxidative stress through Nrf-2 activation

Functional Heterogeneity of Periportal and Perivenous Hepatocytes

Glycogen content in hepatocytes is related with their size in normal rat liver but not in cirrhotic one

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