Routine HLA DRB/DQB oligonucleotide typing by a non-radioactive dot-blot micromethod

García-Pacheco, José Marcos; Mantilla, P.; Olivares, Enrique G.; Manzano-Fernández, M.N.

doi:10.1016/0022-1759(94)00296-9

Cited by 6 publications

(2 citation statements)

References 9 publications

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“…The PCR-SSOP can be set up in two different formats, the product dot blot [98][99][100][102][103][104][105][107][108][109][110][111][112]117,119,[121][122][123][124][125][126][127] and the reverse dot blot [101,106,[113][114][115][116]118]. In the conventional product dot blot approach, the PCR products of different samples are immobilized on a carrier matrix (e.g.…”

Section: Sequence-specific Oligonucleotide Probing (Pcr-ssop)mentioning

confidence: 99%

“…Many alternative methods for conventional product dot blot PCR-SSOP have been described in the literature which differ mainly in the length and sequence of probes, the solvent used for washing steps, the reporter molecule and its detection [98][99][100][102][103][104][105][107][108][109][110][111][112]117,119,[121][122][123][124][125][126][127]. The most relevant product dot blot protocol for serology-equivalent HLA class I and highresolution HLA class II PCR-SSOP typing has been established and extensively evaluated during the 12th IHWC.…”

Section: Sequence-specific Oligonucleotide Probing (Pcr-ssop)mentioning

confidence: 99%

See 1 more Smart Citation

New Diagnostic Methods in Oncology and Hematology

Huhn¹

1998

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Section: Sequence-specific Oligonucleotide Probing (Pcr-ssop)mentioning

confidence: 99%

Section: Sequence-specific Oligonucleotide Probing (Pcr-ssop)mentioning

confidence: 99%

New Diagnostic Methods in Oncology and Hematology

Huhn¹

1998

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New HLA Typing Methods

Blasczyk¹

1998

New Diagnostic Methods in Oncology and Hematology

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Screening for the IDDM high‐risk genotype. A rapid microtitre plate method using serum as source of DNA

Cinek

Wilkinson

Paltiel³

et al. 2000

Tissue Antigens

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Norwegian babies born with the HLA-DRB1*0401-DQA1*03-DQB1*0302/DRB1*03-DQA1+ ++*05-DQB1*0201 genotype have an estimated 17% lifetime risk of developing insulin-dependent diabetes mellitus (IDDM). Identifying these children is important for future prevention, and for studies of the non-genetic factors involved in IDDM. The aim of the study was to develop a rapid screening method for this high-risk genotype. DNA was extracted from serum collected during routine newborn screening for phenylketonuria and hypothyreosis. The second exons of HLA-DQA1 and DQB1 were co-amplified using biotinylated primers, amplicons were hybridized to a set of seven probes immobilized on a microtitre plate using a single hybridisation temperature, and detected colorimetrically by streptavidin-HRP conjugate and tetramethylbenzidine substrate. The DRB1*04 subtyping was performed using six different probes at identical conditions. The prevalence of the DRB1*0401-DQA1*03-DQB1*0302/DRB1*03-DQA1*0 5-DQB1*0201 genotype among 1,026 Norwegian babies was 2.7% (CI 95%: 1.7-3.7%). The new high-throughput genetic screening method for IDDM risk can easily be automated and included in newborn screening programs.

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Routine HLA DRB/DQB oligonucleotide typing by a non-radioactive dot-blot micromethod

Cited by 6 publications

References 9 publications

New Diagnostic Methods in Oncology and Hematology

New Diagnostic Methods in Oncology and Hematology

New HLA Typing Methods

Screening for the IDDM high‐risk genotype. A rapid microtitre plate method using serum as source of DNA

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