Routine Cell Culture

Martin, Bernice M.

doi:10.1007/978-1-4612-0247-9_3

Cited by 3 publications

(3 citation statements)

References 15 publications

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“…Insulin, transferrin, selenium and putrescine are essential factors required for all mammalian cells (Martin, 1994 ;Roh et al, 1994). Basic fibroblast growth factor has a prominent effect on RPE proliferation rate and morphology (Song and Lui, 1990).…”

Section: Discussionmentioning

confidence: 99%

Serum-free Media for Culturing and Serial-Passaging of Adult Human Retinal Pigment Epithelium

Tezel

Priore

1998

Experimental Eye Research

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Section: Discussionmentioning

confidence: 99%

Serum-free Media for Culturing and Serial-Passaging of Adult Human Retinal Pigment Epithelium

Tezel

Priore

1998

Experimental Eye Research

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“…This enzyme is the most frequently used for isolation of MSC due to its ability to cleave collagen connections [27]. The optimum concentration of the enzyme, the incubation time, and the temperature must be carefully monitored during isolation [28].…”

Section: Mesenchymal Stem Cell Culturementioning

confidence: 99%

“…The use of antibiotics is important to prevent contamination, and it is necessary to evaluate the type of contamination that cells may be exposed to and potential toxicity of the dose when choosing which antibiotic to use. The most commonly used antibiotics are penicillin and streptomycin, making an effective and relatively non-toxic combination at the concentrations of 100 U/mL and 100 mg/mL, respectively [28].…”

Section: Mesenchymal Stem Cell Culturementioning

confidence: 99%

Applications of mesenchymal stem cell technology in bovine species

et al. 2019

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Mesenchymal stem cells (MSCs) have received a great deal of attention over the past 20 years mainly because of the results that showed regeneration potential and plasticity that were much stronger than expected in prior decades. Recent findings in this field have contributed to progress in the establishment of cell differentiation methods, which have made stem cell therapy more clinically attractive. In addition, MSCs are easy to isolate and have anti-inflammatory and angiogenic capabilities. The use of stem cell therapy is currently supported by scientific literature in the treatment of several animal health conditions. MSC may be administered for autologous or allogenic therapy following either a fresh isolation or a thawing of a previously frozen culture. Despite the fact that MSCs have been widely used for the treatment of companion and sport animals, little is known about their clinical and biotechnological potential in the economically relevant livestock industry. This review focuses on describing the key characteristics of potential applications of MSC therapy in livestock production and explores the themes such as the concept, culture, and characterization of mesenchymal stem cells; bovine mesenchymal stem cell isolation; applications and perspectives on commercial interests and farm relevance of MSC in bovine species; and applications in translational research.

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Molecular and Cytogenetic Characterization of Fish Cell Lines and its Application in Aquatic Research

Nagpure

Mishra

Ninawe

et al. 2015

Natl. Acad. Sci. Lett.

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Fish cell line has emerged as an important tool in fishery biotechnology. In recent years, various fish cell lines have been developed by different researchers across the country. National Repository on Fish cell lines, established with the aim to preserve fish cell lines for training and education to stakeholders, has started functioning at National Bureau of Fish Genetic Resources, Lucknow. This repository is supposed to characterize and preserve the fish cell lines developed across the country and serve as a national referral centre for Indian and exotic fish cell lines. Currently, the repository is maintaining 50 fish cell lines deposited by various research institutes in India, including the cell lines developed at cell culture facility of National Bureau of Fish Genetic Resources. The cell lines have been successfully cryopreserved after verifying its authenticity by sequence analysis of two mitochondrial genes, viz. 16S rRNA and cytochrome c oxidase sub-unit I. Chromosomal analysis, transfection efficiency and immunocytochemistry are also being used to characterize the cell lines. The facility is serviceable for the collection, deposition and distribution of fish cell lines. This paper discusses the status as well as the methodology adopted for fish cell lines development, characterization and storage at NRFC. Keywords Cell line repository Á Cryopreservation Á Fish cell line Á Molecular characterization Cell culture is the most important means available in life sciences to study the biological activities occurring inside cells of a living body and it refers to the in vitro growth of cells taken from tissue of multicellular organisms. The cells are bathed in a culture medium containing essential nutrients and energy sources necessary for the cells survival [1] The culture environment usually consists of a suitable glass or plastic culture vessel containing a liquid or semisolid medium that supplies nutrients to the growing cells. Fish cell lines have increased tremendously in number (283), since the development of the first permanent fish cell

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Routine Cell Culture

Cited by 3 publications

References 15 publications

Serum-free Media for Culturing and Serial-Passaging of Adult Human Retinal Pigment Epithelium

Serum-free Media for Culturing and Serial-Passaging of Adult Human Retinal Pigment Epithelium

Applications of mesenchymal stem cell technology in bovine species

Molecular and Cytogenetic Characterization of Fish Cell Lines and its Application in Aquatic Research

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