Routine application of enzyme-linked immunosorbent assay in comparison with complement fixation for the diagnosis of foot-and-mouth and swine vesicular diseases

Ferris, N.P.; Dawson, M.

doi:10.1016/0378-1135(88)90024-7

Cited by 216 publications

(147 citation statements)

References 12 publications

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“…Virus isolation was attempted from all samples in either primary bovine thyroid cell cultures for all epithelial and oropharyngeal samples or renal swine cell cultures (RS) for epithelial and pig whole-blood samples by the WRL standard protocol (18). All cultures showing FMDV cytopathic effect were harvested and serotyped with the WRL indirect sandwich enzyme-linked immunosorbent assay (12,18,27).…”

Section: Methodsmentioning

confidence: 99%

Molecular Epidemiology of Foot-and-Mouth Disease Viruses in the Adamawa Province of Cameroon

et al. 2004

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Foot-and-mouth disease virus (FMDV) causes a highly contagious viral disease of even-toed ungulates and is one of the most important economic diseases of livestock. Most studies of FMDV are done in countries where control measures are being implemented. In contrast, in areas such as sub-Saharan Africa, where FMDV is endemic and new strains are likely to emerge, there are only sporadic submissions to the World Reference Laboratory, Pirbright, United Kingdom. This paper describes the molecular epidemiology of FMDV in the Adamawa province of Cameroon based on a population sample of cattle herds. Serotypes SAT2 and A were isolated in the cross-sectional study. SAT2 isolates were all similar, with phylogenetic distances of <6%, and were most closely related to published sequences of isolates from Eritrea and Saudi Arabia. Serotype A isolates were more variable, with phylogenetic distances of 0 to 11%, and were most closely related to historic isolates from Cameroon. Use of a population-based sample gives a representative sample of virus diversity and will improve our understanding of the evolution of FMDV and its epidemiology. A supplementary study of pigs passing through the railhead collection yard at Ngaoundere detected a serotype O virus. A third pilot longitudinal study monitored viral persistence in three cattle herds over 12 months, and serotype O and A viruses were recovered from a herd 12 months after it was first recorded as being infected with SAT2 virus. The pig type O isolate was not closely related to that recovered from the cattle, suggesting that the pigs had not introduced the O virus into the cattle herds.

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Section: Methodsmentioning

confidence: 99%

Molecular Epidemiology of Foot-and-Mouth Disease Viruses in the Adamawa Province of Cameroon

et al. 2004

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“…The tubes were subsequently rolled continuously at 37 °C and the cell cultures examined microscopically for evidence of a cytopathic effect (CPE) daily for 3 days post inoculation, and recorded as positive or negative. The FMDV specificity of random samples producing a CPE was confirmed by ELISA [11] and the titre of the virus stock was expressed as 50% tissue culture infective doses (TCID 50 /mL; [13]). …”

Section: Virus Isolationmentioning

confidence: 99%

“…In contrast, the antigen-detection ELISA [11] currently used at the FAO World Reference Laboratory for FMD, Pirbright, is unsuitable for testing milk. Previous studies have demonstrated that automated realtime RT-PCR (rRT-PCR) is a valuable diagnostic tool for the laboratory detection of FMDV in vesicular epithelial tissue [15,16] and in serum, nasal swabs and oesophageal-pharyngeal scraping ("probang") samples [20].…”

Section: Introductionmentioning

confidence: 99%

Utility of automated real-time RT-PCR for the detection of foot-and-mouth disease virus excreted in milk

et al. 2006

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-Foot-and-mouth disease virus (FMDV) can be excreted in milk and thereby spread infection to susceptible animals in other holdings. The feasibility of using real-time reverse transcription polymerase chain reaction (rRT-PCR) as a diagnostic tool for detection of FMDV in milk was assessed by studying the excretion of virus from experimentally-infected cattle. Fore-and machine milk samples were collected over a 4-week period from two dairy cows infected with FMDV and from two in-contact cows held in the same pen. The whole, skim, cream and cellular debris components of the milks were tested by automated rRT-PCR and results compared to virus isolation (VI) in cell culture. The onset of clinical signs of FMD in all four cows correlated with viraemia, and the presence of FMDV in other clinical samples. rRT-PCR results matched closely with VI in detecting FMDV in all milk components and generally coincided with, but did not consistently precede, the onset of clinical signs. rRT-PCR detected FMDV in milk up to 23 days post inoculation which was longer than VI. Furthermore, the detection limit of FMDV in milk was greater by rRT-PCR than VI and, in contrast to VI, rRT-PCR detected virus genome following heat treatment that simulated pasteurisation. rRT-PCR was also able to detect FMDV in preservativetreated milk. In conclusion, this study showed that automated rRT-PCR is quicker and more sensitive than VI and can be used to detect FMDV in whole milk as well as milk fractions from infected animals. milk / milk components / foot-and-mouth disease virus / real-time RT-PCR / pasteurisation

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“…Virus isolation and titration. The infectivity of oropharyngeal fluid samples was measured by inoculation of monolayers of primary calf thyroid (BTY) cells, essentially as described by Ferris & Dawson (1988). Five BTY tubes were used for each dilution.…”

Section: Probang Antibody Measurementmentioning

confidence: 99%

Evidence of partial protection against foot-and-mouth disease in cattle immunized with a recombinant adenovirus vector expressing the precursor polypeptide (P1) of foot-and-mouth disease virus capsid proteins.

Sanz-Parra

V√°zquez

Sánchez‐Madrid

et al. 1999

Journal of General Virology

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A recombinant live vector vaccine was produced by insertion of cDNA encoding the structural proteins (P1) of foot-and-mouth disease virus (FMDV) into a replication-competent human adenovirus type 5 vaccine strain (Ad5 wt). Groups of cattle (n l 3) were immunized twice, by the subcutaneous and/or intranasal routes, with either the Ad5 wt vaccine or with the recombinant FMDV Ad5-P1 vaccine. All animals were challenged by intranasal instillation of FMDV 4 weeks after the second immunizations. In the absence of a detectable antibody response to FMDV, significant protection against viral challenge was seen in all of the animals immunized twice by the subcutaneous route with the recombinant vaccine. The observed partial protection against clinical disease was not associated with a reduction in titre of persistent FMDV infections in the oropharynx of challenged cattle.

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Routine application of enzyme-linked immunosorbent assay in comparison with complement fixation for the diagnosis of foot-and-mouth and swine vesicular diseases

Cited by 216 publications

References 12 publications

Molecular Epidemiology of Foot-and-Mouth Disease Viruses in the Adamawa Province of Cameroon

Molecular Epidemiology of Foot-and-Mouth Disease Viruses in the Adamawa Province of Cameroon

Utility of automated real-time RT-PCR for the detection of foot-and-mouth disease virus excreted in milk

Evidence of partial protection against foot-and-mouth disease in cattle immunized with a recombinant adenovirus vector expressing the precursor polypeptide (P1) of foot-and-mouth disease virus capsid proteins.

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