Round cell variant of measles virus: Spontaneous conversion from productive to cell-associated state of infection

Carrigan, Donald R.

doi:10.1016/0042-6822(85)90276-4

Cited by 11 publications

(6 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Therefore, the hypothesis can be made that nonproductive, cell-associated forms of measles virus occur naturally (11), that they commonly infect the CNS during rubeola (18), and that they are usually suppressed by host defensive mechanisms, especially IFN. SSPE may occur when such suppression partially fails, which allows the virus to express low levels of its polypeptides and to slowly spread within the CNS.…”

mentioning

confidence: 99%

Identification of a nonproductive, cell-associated form of measles virus by its resistance to inhibition by recombinant human interferon

Carrigan¹,

Kabacoff²

1987

J Virol

View full text Add to dashboard Cite

Subacute sclerosing panencephalitis (SSPE) is a fatal disease in children and young adults that is caused by persistent infection of the central nervous system (CNS) by a nonproductive, cell-associated form of measles virus. Using an experimental model for SSPE (LEC viral strain in newborn hamsters), we have shown previously that establishment of such CNS infections involves selective elimination from the CNS of productively infected cells by host defensive mechanisms, coupled with the selective sparing of cells carrying nonproductive viral forms. That interferon (IFN) may play a role in this process was suggested by the disappearance of productively infected cells from the CNS tissues prior to the appearance of antiviral antibodies and by the demonstration of cell-associated, IFN-resistant viral variants in the virus stocks that were used. Results of this study support these conclusions by showing that similar IFN-resistant viral variants are present in the HBS strain of SSPE-derived measles virus and that these variants, in the presence of IFN, have properties that are similar to those of naturally occurring cell-associated'strains of SSPE viruses, e.g., DR, IP3, and Biken. These IFN-resistant forms of HBS virus were isolated and were shown to maintain their resistance to inhibition by IFN after cloning. However, on removal of IFN, they reverted to productive forms similar to the parental HBS virus. The potential role of such viral forms in the pathogenesis of SSPE is discussed.

show abstract

mentioning

confidence: 99%

Identification of a nonproductive, cell-associated form of measles virus by its resistance to inhibition by recombinant human interferon

Carrigan¹,

Kabacoff²

1987

J Virol

View full text Add to dashboard Cite

show abstract

“…This virus was derived from the LEC strain of SSPE measles virus [Barbanti-Brodano et al, 19701 and consists of two separate, but interconvertible, viral variants with different biological properties. These variants have been described in detail [Carrigan, 1985;Carrigan, 19861.…”

Section: Introductionmentioning

confidence: 99%

Chronic, relapsing encephalomyelitis associated with experimental measles virus infection

Carrigan

1987

Journal of Medical Virology

Self Cite

View full text Add to dashboard Cite

An experimental central nervous system (CNS) disease that bears some similarity to human multiple sclerosis and that is associated with infection by measles virus has been induced in hamsters. This disease has been described previously (Carrigan and Johnson: Proceedings of the National Academy of Sciences 77:4297-4300, 1980). At that time, it was believed that the disease was restricted to the spinal cords of affected animals. The present work demonstrates that the disease can also affect the brain, describes in more complete detail the types of clinical disease that occur, illustrates the histopathological changes found in diseased CNS tissues, and documents more accurately the incidence of the disease in the virally infected animals. In summary, the disease, which has now been termed chronic, relapsing encephalomyelitis (CRE) is associated with neonatal CNS infection of hamsters with a particular strain of measles virus. CRE occurs in approximately 12% of animals that survive the acute viral infection, and of these affected animals about half develop detectable clinical signs of neurological disease. The balance of the animals have subclinical disease detectable only by histopathologic changes within the CNS. These lesions are composed of varying degrees of demyelination, necrosis, mononuclear cell inflammation, and gliosis.

show abstract

“…Certain strains can induce different types of c.p.e. (Carrigan, 1985) and other strains have a low haemagglutinating capacity (Marusyk & Tyrrell, 1984). This work adds another variable, the capacity to infect and suppress functions of lymphoid cells, to the known biological variations of measles virus.…”

Section: Ipmentioning

confidence: 99%

Measles Virus Strain-dependent Variation in Outcome of Infection of Human Blood Mononuclear Cells

Ilonen

Salonen

Marusyk

et al. 1988

Journal of General Virology

View full text Add to dashboard Cite

SUMMARYEight measles virus strains including four subacute sclerosing panencephalitis (SSPE) isolates were compared on the basis of their growth characteristics in human peripheral blood mononuclear cells and effect on mitogen-stimulated lymphocyte proliferation. The Edmonston strain virus and some other strains of measles virus replicated in phytohaemagglutinin (PHA)-stimulated lymphocytes and released high titres of infectious virus into the culture medium. Inhibition of DNA synthesis was relatively low at the beginning of lymphocyte proliferation and could be detected only at high multiplicity of infection. Three to 4 days after initiation of proliferation a strong inhibition was seen also in cultures infected with low doses of virus, and was apparently related to the production of high titres of new infectious virus. Some virus strains originally isolated from SSPE patients produced only a small amount of infectious virus in lymphocytes at 37 °C but had a very strong inhibitory effect on lymphocyte proliferation leading to early cell death. The inhibitory effect was found over a wide range of virus concentrations, but was strictly dose-dependent and no increase in inhibition with low multiplicities of infection could be seen with longer culture times. The amount of interferon induced by different strains varied from 400 to 6400 international units/ml but the amount of interferon produced in the cultures did not correlate with the inhibitory effect on proliferation or with the amount of released new infectious virus. The Hall6 measles strain, originally isolated from an SSPE patient, differed most from the Edmonston strain in its characteristics, and was studied in greater detail. Although only a small amount of infectious virus was produced, the Hall6 strain had a very strong inhibiting effect on the proliferation of PHA-stimulated lymphocytes, and extensive syncytium formation was seen leading to cell death within 3 days.

show abstract

Round cell variant of measles virus: Spontaneous conversion from productive to cell-associated state of infection

Cited by 11 publications

References 41 publications

Identification of a nonproductive, cell-associated form of measles virus by its resistance to inhibition by recombinant human interferon

Identification of a nonproductive, cell-associated form of measles virus by its resistance to inhibition by recombinant human interferon

Chronic, relapsing encephalomyelitis associated with experimental measles virus infection

Measles Virus Strain-dependent Variation in Outcome of Infection of Human Blood Mononuclear Cells

Contact Info

Product

Resources

About