Rotten-skin disease significantly changed giant spiny frog(Paa spinosa) gut microbiota

Abstract

The composition and abundance of gut microbiota is essential for host health 22 and immunity. Gut microbiota is symbiotic with the host, so changes in the host diet, 23 development, and health will lead to changes in the gut microbiota. Conversely, changes 24 in the gut microbiota also affect the host conditions. In this experiment, 16S rRNA high-25 throughput sequencing was used to compare the gut microbiota composition of 5 26 healthy Paa Spinosa and 6 P. spinosa with rotten-skin disease. Results: the gut 27 microbiota composition was significant difference between diseased P. spinosa and the 28 healthy P. spinosa; LEfSe analysis showed that the relative abundance of 29 Methanocorpusculum, Parabacteroides, AF12, PW3, Epulopiscium, and Oscillospira 30 were significantly higher in the diseased P. spinosa, while the relative abundance of 31 Serratia, Eubacteium, Citrobacter, and Morganella were significantly lower. 32 Conclusion: Rotten-skin disease changed P. spinosa gut microbiota significantly; The 33 relative abundance of Epulopiscium and Oscillospira might be related to the health 34 conditions of the host skin and gallbladder; The relative abundance of Serratia and 35 Eubacteium might be important for maintaining the gut microbiota ecosystem. 36 37 38 39 40 41

The Circadian Rhythm of the Behavior and Gut Microbiota in Dybowski’s Frogs (Rana dybowskii) during the Autumn Migration Period

“…Research on Asiatic toads ( Bufo gargarizans ) has revealed considerable structural differences between the large and small intestines [ 26 ]. Moreover, rotten-skin disease is shown to significantly alter the gut microbiota of the giant spiny frog ( Paa spinosa ) [ 27 ]. As a variable temperature vertebrate, the larvae (tadpoles) of frogs live in water, while the majority of adults live on land.…”