Rotifers in Ecotoxicology

Rico‐Martínez, Roberto; Pérez‐Legaspi, Ignacio Alejandro; Arias-Almeida, Juan Carlos; Santos-Medrano, Gustavo Emilio

doi:10.1007/978-94-007-5704-2_89

Cited by 10 publications

(7 citation statements)

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“…The total number of rotifers in each well was counted on the 5th day and used to calculate the net reproductive rate (r, offspring per rotifer per day) for each treatment, using the formula r = (ln N t – ln N 0 )/T . ln N t is the natural log of the number of Philodina present after 5 days, ln N 0 is the natural log of the initial number of Philodina in the well, and T is 5 days. For a test to be valid, control treatments should have an average of at least 10 rotifers per well after 5 days.…”

Section: Methodsmentioning

confidence: 99%

“…Interest in rotifers as tools for assessing toxicity in aquatic environments continues to increase. Several review papers have appeared in the last few years highlighting the activity in this research area . These summarize the broad range of approaches from molecular and physiological to behavioral and population endpoints being used to characterize toxicity.…”

Section: Introductionmentioning

confidence: 99%

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Freshwater toxicity testing using rehydratedPhilodinasp. (Rotifera) as test animals

Snell

Johnston

Matthews

2017

Environmental Toxicology

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Rotifers have become widely used in aquatic toxicology as a rapid screening test for toxicity. The commercial availability of diapausing embryos (cysts) have facilitated their popularity because test animals can be obtained without having to master the details of culturing. Other rotifer species have life stages capable of surviving desiccation and also could be used in non-culture systems for toxicity assessment. In this article, we describe a system for toxicity testing in freshwater based on rehydrating desiccated bdelloid rotifers in the genus Philodina. These animals can remain in this anhydrobiotic state for more than one year and then rehydrate within hours to provide animals for toxicity tests. We describe three endpoints: a 1.5 h ingestion test, a 24 h mortality test, and a five day reproductive test. The latter test requires feeding and a method using a dried commercial product is explained. Using desiccated rotifers and dried food in toxicity tests make this system especially attractive because of its flexibility and low threshold of biological expertise required to execute the tests. The use of the Philodina toxicity test is illustrated with four metals: copper, lead, mercury and cadmium. Reproduction generally was the most sensitive endpoint, with EC50s of 0.33, 0.44, 0.60, and 0.12 mg/L, respectively. Ingestion was a close second with EC50s of 0.13, 1.64, 0.64, and 6.26 mg/L, respectively.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Freshwater toxicity testing using rehydratedPhilodinasp. (Rotifera) as test animals

Snell

Johnston

Matthews

2017

Environmental Toxicology

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“…For example, in B. calyciflorus and B. plicatilis the LC50 value is 4.0 mg L -1 , while in B. patulus it is 6.15. In contrast, in L. quadridentata the LC50 it is 3.7 mg L -1 , in L. hamata it is 0.68, and in L. luna it is 0.14 mg L -1 [11]. It seems by looking at the small set of data that the Brachionus species are more tolerant to lead than species of Lecane.…”

Section: Resultsmentioning

confidence: 89%

Bioconcentration of Lead and x-ray Microanalysis with SEM in the Freshwater Rotifer Lecane quadridentata (Rotifera: Monogononta)

Alvarado-Flores¹,

Rico‐Martínez²

2017

Pol. J. Environ. Stud.

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“…The ability to produce cysts has allowed the development of toxicity kits, called Rotoxkits, employed for acute/chronic marine and freshwater toxicity testing [179]. Cyst production is an outstanding characteristic that has enabled the development of several toxicity protocols using rotifers that have been used worldwide [180]. Rotifers are not directly represented in the legislation of several countries (as the cladocerans Daphnia magna and Ceriodaphnia dubia do).…”

Section: Rotifer Tests Already Used Worldwide and In Certain Regionsmentioning

confidence: 99%

Rotifers as Models in Toxicity Screening of Chemicals and Environmental Samples

Rico‐Martínez¹,

Arzate-Cárdenas²,

Robles-Vargas³

et al. 2016

Invertebrates - Experimental Models in Toxicity Screening

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An important objective of aquatic ecotoxicology is to determine the effects of toxic compounds in organisms that play a central role in aquatic communities where rotifers have a large impact on several important ecological processes. The contribution of the rotifers to secondary production in many aquatic communities is substantial as they are often the larger fraction of zooplankton biomass at certain times of the year. In addition to the importance of their ecological roles in aquatic communities, the rotifers are attractive organisms for ecotoxicological studies by its short life cycles and rapid reproduction, their small size, and little volumes needed for culture and toxicity assays. The main end points used in ecotoxicological studies are mortality, reproduction, behavior, and biomarkers. Such parameters are included in international regulations from all over the world, where different species are used to evaluate the effect of environmental samples or chemical compounds. The high diversity of rotifers is an important issue because it can modify their relative susceptibility to toxicants. Thus, more studies are needed to know the relations and mechanisms involved in clonal variation, sensitivity, and development, which can be all assessed by state-of-the-art procedures.

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Rotifers in Ecotoxicology

Cited by 10 publications

References 0 publications

Freshwater toxicity testing using rehydratedPhilodinasp. (Rotifera) as test animals

Freshwater toxicity testing using rehydratedPhilodinasp. (Rotifera) as test animals

Bioconcentration of Lead and x-ray Microanalysis with SEM in the Freshwater Rotifer Lecane quadridentata (Rotifera: Monogononta)

Rotifers as Models in Toxicity Screening of Chemicals and Environmental Samples

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