Rotaviruses in Venezuelan Children with Gastroenteritis

Electron Microscopy of Tubular Assemblies Associated with Naturally Occurring Bovine Rotavirus

“…Longitudinal striations are evident in the human tubes (Esparza et al, 1977;Kimura & Murakami, 1977) but this lattice direction is not present in the bovine tubes. The apparent difference may, however, be simply a result of gross distortion of the flattened human particles, and more detailed investigation is indicated.…”

“…Many samples in which rotaviruses are found also contain tubular forms. These tubes, reported in material from human (Flewett et al, 1974;Holmes et al, 1975;Woode et al, 1976;Esparza et al, 1977;Kimura & Murakami, 1977), bovine, equine and murine (Woode et al, 1976) sources, have diameters of about 75 nm to 80 nm which is comparable with that of the associated rotavirus particles, and are presumed to be aberrant assemblies of virus protein, similar in nature to, for example, papilloma virus tubes (Breedis et al, 1962) and the T4 bacteriophage 'polyheads' (Epstein et al, 1963). In addition, agglutination in vitro of the tubes with rotavirus-specific antiserum has indicated that they are composed of proteins related to the virus particles (Flewett et al, 1974;Kimura & Murakami, 1977).…”

The 30‐ to 54‐nm rotavirus‐like particles in gastroenteritis: Incidence and antigenic relationship to rotavirus

“…The 54-nm particles found in patients 10 and 11 most probably represent homogeneous populations of singleshelled or "rough" particles of the classic rotavirus, since both the inner core and overall diameters were compatible with measurements given for rotavirus. This phenomenon has not been described in any of the 1,707 stool specimens reported by laboratories where ultrastructural details of rotavirus particles were given or where the rotavirus particles were described as being "typical" [Flewett et al, 1973;Bishop et al, 1974;Flewett et al, 1974;Holmes et al, 1974;Bryden et al, 1975;Davidson et al, 1975;Schoub et al, 1975;Albrey and Murphy, 1976;Kapikian et al, 1976;Von Bonsdorff et al, 1976;Birch et al, 1977;Esparza et al, 1977;Madeley et al, 1977;McLean et al, 1977;Middleton et al, 1977;: Chrystie et al, 1978Esparza and Gil, 1978;Viera de Torres et al, 1978;Goldwater, 1979;Taraska et al, 19791. When even a few double-shelled particles are present in a stool specimen, the identification of the smaller particles as probable single-shelled rotavirus particles becomes apparent and no "new" candidate viruses are considered.…”

“…Ever since Kapikian et a1 [1972] identified a 27-nm particle as the etiological agent of Norwalk gastroenteritis, electron microscopy has revealed a number of other gastroenteritis-associated viruses in human stool specimens [Flewett et al, 1973;Appleton and Higgins, 1975;Caul and Clarke, 1975;Davidson et al, 1975;Madeley and Cosgrove, 1975;Patterson et al, 1975;Schoub et al, 1975;Caul et al, 1977;Middleton et al, 1977;McLean et al, 1977;Kjeldsberg, 1977;Madeley et al, 1977;Echeverria et al, 1977;Appleton et al, 1977;Cameron et al, 1978;Spratt et al, 1978;Chiba et al, 1979;Suzuki et al, 1979;Taraska et al, 1979;Taniguchi et al, 1979;Puel et al, 1979;Goldwater, 19791, including the common-ly described rotavirus [Bishop et al, 1974;Flewett et al, 1974;Holmes et al, 1974;Kapikian et al, 1974;Middleton et al, 1974;White et al, 1974;Bryden et al, 1975;Holmes et al, 1975;Murphy et al, 1975;Albrey et al, 1976;Kapikian et al, 1976;Totterdell et al, 1976;Von Bonsdorff et al, 1976;Birch et al, 1977;Esparza et al, 1977;Murphy et al, 1977;Portnoy et al, 1977;Rodriguez et al, 1977;<...>…”

A reação de fixação do complemento na identificação de rotavírus humano

“…Dentre esta já longa série de vírus, os rotavírus surgem como relacionados com a etiologia de mais da metade de todos os casos de gastroenterites infantis que ocorrem durante os meses de inverno 8 . Nos chamados países em desenvolvimento as gastroenterites por rotavírus parecem constituir-se num quadro de relativa freqüência e, provavelmente, de marcada importância 1,2,6,9,15,22,26 . Um diagnóstico laboratorial rápido e simples das gastroenterites por rotavírus adquire, assim, um interesse muito particular.…”