Rotavirus serotypes by serum neutralisation

Beards, G. M.; Pilfold, J. N.; Thouless, Margaret E.; Flewett, T. H.

doi:10.1002/jmv.1890050307

Cited by 119 publications

(90 citation statements)

References 9 publications

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“…Several subgroups Greenberg et al 1983) and serotypes (Beards et al 1980;Wyatt et al 1982) have been identified by complement fixation tests (Thouless et al 1977;Zissis & Lambert, 1978), ELISA techniques (Yolken et al 1978;Thouless, Beards & Flewett, 1982) and neutralization assays (Beards et al 1980;Wyatt et al 1982).…”

Section: Introductionmentioning

confidence: 99%

Molecular epidemiology of human rotaviruses: Analysis of outbreaks of acute gastroenteritis in Glasgow and the west of Scotland 1981/82 and 1982/83

Follett

Sanders

Beards

et al. 1984

J. Hyg.

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SUMMARYThe molecular epidemiology of rotavirus infections in Glasgow and the west of Scotland during 1981/82 and 1982/83 was investigated by electron microscopy, ELISA testing and RNA migration pattern analysis. In 1981/82, rotaviruses of both the 'long' and the 'short 'electropherotype (in different variants) co-circulated from the onset throughout the winter peak of the outbreak. Approximately 80 o of the children were infected during the first year of life. No differences in incidence were found between sexes. In 1982/83 the isolated rotaviruses were almost exclusively of the 'long' electropherotype (in different variants) and 360 of the children were infected beyond the first year of life. Rotaviruses of the 'long' electropherotype serologically were of subgroup ii and serotype 1 and those of the 'short' electropherotype of subgroup i and serotype 2.

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Section: Introductionmentioning

confidence: 99%

Molecular epidemiology of human rotaviruses: Analysis of outbreaks of acute gastroenteritis in Glasgow and the west of Scotland 1981/82 and 1982/83

Follett

Sanders

Beards

et al. 1984

J. Hyg.

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“…Recent studies on rotavirus have shown that there are several serotypes in human and avian rotaviruses discriminated by serum neutralization (SN) tests (2,5,11,13). Although considerable attention has been paid to proving the existence of rotaviral serotypes in other species (4), comparatively little is known about those of bovine rotaviruses.…”

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confidence: 99%

“…They suggest that there may be serotypes in bovine rotaviruses by the SN test, as in human and avian rotaviruses (2,5,11,13). It is another point of interest that even in the same herd suffering from diarrhea, the serum showed different neutralization patterns against the isolates.…”

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confidence: 99%

Neutralizing Patterns of Anti‐Bovine Rotavirus (Lincoln) Serum against Cytopathic Bovine Rotaviruses Isolated from Calves in Japan

Murakami

Nishioka

Hashiguchi

et al. 1981

Microbiology and Immunology

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Recent studies on rotavirus have shown that there are several serotypes in human and avian rotaviruses discriminated by serum neutralization (SN) tests (2,5, 11,13). Although considerable attention has been paid to proving the existence of rotaviral serotypes in other species (4), comparatively little is known about those of bovine rotaviruses. There is only one report that distinguished two bovine rotavirus strains by the hemagglutination inhibition (HI) test (9). Since bovine rotavirus strains do not always possess hemagglutinating properties (3, 9), it is difficult to distinguish serotypes in them by the HI test.The present report gives the results of SN and complement fixation (CF) tests with antiserum against Lincoln strain of bovine rotavirus when these tests were applied to the cytopathic bovine rotaviruses isolated from the feces of diarrheic calves in Japan.The Lincoln strain at the 17th passage in bovine kidney cells (7) was kindly supplied by Dr. M. Kodama, of the authors' institute. It was further passaged three times in a continuous line of fetal rhesus monkey kidney (MA 104) cells in the presence of trypsin (Type 1, Sigma Chemical Co.) at the authors' laboratory.Isolation and electron microscopic detection of rotaviruses were performed in essentially the same manner as described by Babiuk et al (1). Briefly, the saline extracts of feces obtained from diarrheic calves were treated with 10 ,ug/ml of trypsin at 37 C for 30 min and inoculated into eight tube cultures of MA 104 cells washed with Earle's solution, in 0.1 ml of a ten-fold serial dilution. Incubation was carried out in the presence of 1 ,ug/ml of trypsin. The tube culture with intense cytopathic effect (CPE) at the end of the serial dilution was used for more than three passages by the same method as described above. Fourteen cytopathic bovine rotaviruses were isolated from different calves of seven herds suffering from diarrhea, and identified by the CF test and electron microscopy (EM). The propagation of MA 104 cells was performed in the manner previously described (6). Viral titration and the SN test were also carried out with MA 104 cells. For the titration, 0.1 ml of ten-fold serial dilution of each virus was inoculated into four tube cultures washed with Earle's solution. After viral adsorption at 37 C for 1097

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“…It showed a two-way cross-reaction with B R V E4046 and a one-way crossreaction with BRV B223. However, cross-neutralization of BRV V1005 with B223-specific rabbit serum was not observed with the corresponding guinea-pig serum and cross-neutralization of BRV B223 with the V1005- * As determined in the fluorescent focus neutralization test (Beards et al, 1980). t The neutralization titre of UK/7 was > 10000 against both BRV UK and BRV NCDV.…”

Section: Discussionmentioning

confidence: 97%

“…The injections were repeated 14 days later and the rabbits were bled by cardiac puncture 7 to 10 days after the second injection. Neutralization of fluorescent focus production in MA-104 cells was used, essentially as described by Beards et al (1980). Titres are expressed as the reciprocal of the serum dilution reducing fluorescent foci by 50%…”

confidence: 99%

Antigenic and biochemical characterization of bovine rotavirus V1005, a new member of rotavirus serotype 10

Brüssow

Snodgrass

Fitzgerald

et al. 1990

Journal of General Virology

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Bovine rotavirus (BRV) V1005 is serologically distinct from rotavirus serotypes 1, 2, 3, 4, 5, 6, 8 and 9. BRV V1005 showed cross-reactions with BRV B223, the American prototype of serotype 10 rotavirus, and with BRV E4049, a British serotype 10 isolate. BRV V1005 was, however, not neutralized by four monoclonal antibodies directed against VP7 of BRV B223. Twoway cross-reactions were observed between BRV V1005 and a reassortant rotavirus containing the VP4 from BRV UK. In addition the major tryptic cleavage product of VP4, VP5*, from BRV V1005 is indistinguishable by peptide mapping and its isoelectric point from the homologous protein of BRV UK, hut is clearly different from VP5* of BRV NCDV. The peptide map of VP7 from BRV V1005 differed from that obtained for VP7 of BRV UK.

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Rotavirus serotypes by serum neutralisation

Cited by 119 publications

References 9 publications

Molecular epidemiology of human rotaviruses: Analysis of outbreaks of acute gastroenteritis in Glasgow and the west of Scotland 1981/82 and 1982/83

Molecular epidemiology of human rotaviruses: Analysis of outbreaks of acute gastroenteritis in Glasgow and the west of Scotland 1981/82 and 1982/83

Neutralizing Patterns of Anti‐Bovine Rotavirus (Lincoln) Serum against Cytopathic Bovine Rotaviruses Isolated from Calves in Japan

Antigenic and biochemical characterization of bovine rotavirus V1005, a new member of rotavirus serotype 10

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