Rotavirus serotypes 6 and 10 predominate in cattle

Abstract

Calf fecal rotavirus strains were serotyped in enzyme-linked immunosorbent assays, using monoclonal antibodies to the VP7s of serotypes 1, 2, 3, 5, and 6 and to the VP4 of B223 (designated serotype 10). Sixty-six percent of 162 samples were typed as serotype 6, and 7% were serotyped as serotype 10. Most of the untyped strains did not react with a monoclonal antibody directed to a common VP7 epitope, indicating insufficient virus present in the samples. However, seven untyped samples that did react with this antibody were adapted to culture and typed, and six of these also proved to belong to serotype 6 or 10. Two of these viruses belonged to a monotype within serotype 6 that did not react with the serotype 6 monoclonal antibody. The seventh isolate reacted in cross-neutralization tests with serotype 8 viruses. Bovine rotaviruses from the United Kingdom, Federal Republic of Germany, and Japan that had been shown previously to be distinct from serotype 6 were compared in neutralization tests with B223 from the United States. These viruses proved to be a closely reacting group distinct from all other rotavirus serotypes, justifying the establishment of serotype 10 as the second major type of bovine rotavirus.

Serological and genotypic characterization of group a rotavirus reassortants from diarrheic calves born to dams vaccinated against rotavirus

“…At least 8 different BRV G-types have been associated with neonatal calf diarrhea (Mebus et al 1971;Woode et al, 1983;Matsuda et al, 1990, Snodgrass et al, 1984, Snodgrass et al, 1990Hussein et al 1993;Parwani et al, 1993). In the United States, type G6 appears to be most widespread, with type G10 accounting for a lower percentage of infections ~ .…”

Ovine rotavirus strain LLR-85-based bovine rotavirus candidate vaccines: Construction, characterization and immunogenicity evaluation

“…Today rotaviral infections are proven to be a common, economically important cause of calf diarrhea throughout the world. Genotypic analyses of a major neutralization protein VP7 of a bovine rotavirus in diarrheal stools collected in different cattle populations throughout the world have shown that the majority of typeable rotavirus isolates belong to G genotypes 6 and 10 (Alfieri et al, 2004;Chang et al, 1996;Falcone et al, 1999;Garaicoechea et al, 2006;Monini et al, 2008;Parwani et al, 1993;Reidy et al, 2006;Snodgrass et al, 1990). Since the first licensed bovine rotavirus vaccine was a monovalent one, monovalent rotavirus vaccine cannot completely protect against heterologous rotavirus infection (Feng et al, 1994).…”

“…Field surveys have demonstrated that, so far, G genotype G6 and G10 are the epidemiologically most important bovine rotavirus genotypes worldwide, accounting for 90% of the rotavirus-caused diarrhea approximately. Next to the G6 and G10 genotypes, G8 is the third typical bovine rotavirus genotype (Alfieri et al, 2004;Chang et al, 1996;Falcone et al, 1999;Garaicoechea et al, 2006;Monini et al, 2008;Parwani et al, 1993;Reidy et al, 2006;Snodgrass et al, 1990). In China, our epidemiologic surveillance has shown that rotavirus diarrhea is one of the most important diseases of neonatal calves, and that G6 and G10 were also determined to be the most prevalent genotype of the bovine rotaviruses using nested RT-PCR and sequencing (Chang et al, 2008).…”

Chapter 8 Control of intestinal diseases by dietary supplementation with antibodies

“…Orally administered specific antibodies against enteropathogenic viruses block the development of diarrhea resulting from infections with rota-and coronaviruses (Besser et al, 1988;Snodgrass et al, 1990;Heckert et al, 1999;Erhard et al, 1993;Ikemori et al, 1997).…”