Rotavirus NSP4: A Multifunctional Viral Enterotoxin

Ball, Joseph A.; Mitchell, Deanne M.; Gibbons, Thomas F.; Parr, Rebecca D.

doi:10.1089/vim.2005.18.27

Cited by 85 publications

(79 citation statements)

References 128 publications

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“…These changes occur at amino acid positions 131 and 133, which are in the region (amino acid 131-140) reported to be responsible for altered pathogenesis mediated by the NSP4 protein (Zhang et al, 1998). Also observed are amino acid substitutions in the four previously described NSP4 antigenic sites (Ball et al, 2005). Antigenic sites AS IV, AS III, AS II and AS I had 1, 1, 5, 3 amino acids substitutions, respectively, when compared with strains in the E1 genotype.…”

Section: Discussionmentioning

confidence: 74%

“…Three strains exhibited changes at position H131Y and the other four at position N133S. Amino acid substitutions were observed within previously described antigenic sites (Ball et al, 2005;Borgan et al, 2003): ASIV (aa 1-24) at position L7 V; ASIII (aa 112-133) at positions H131Y and N133S; ASII (aa136-150) at positions I136A, P138S, V139A, V141I and I142V and ASI (151-169 aa) at positions E160N, S161N, and L169I when aligned with other E1 genotype strains (Fig. 4).…”

Section: Analysis Of Nsp4 Nucleotide Sequencesmentioning

confidence: 72%

“…The nucleotide (amino acid) identity among Cameroonian strains ranged between 90.9%-100% (94.3%-100%). Analysis of deduced amino acid sequences of Cameroonian strains and rotaviruses detected worldwide demonstrated that seven of the fifteen strains detected in Cameroon showed an amino acid substitution within the enterotoxin domain (aa 114-135) (Ball et al, 2005). Three strains exhibited changes at position H131Y and the other four at position N133S.…”

Section: Analysis Of Nsp4 Nucleotide Sequencesmentioning

confidence: 99%

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Human G9P[8] rotavirus strains circulating in Cameroon, 1999–2000: Genetic relationships with other G9 strains and detection of a new G9 subtype

Esona

Mijatovic-Rustempasic

Foytich

et al. 2013

Infection, Genetics and Evolution

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Group A rotaviruses (RV-A) are the leading cause of viral gastroenteritis in children worldwide and genotype G9P[8] is one of the five most common genotypes detected in humans. In order to gain insight into the degree of genetic variability of G9P [8] HHS Public Access BackgroundChildhood mortality has been declining worldwide as a result of socioeconomic development and implementation of prevention and survival interventions (Claeson et al., 2000). Group A rotaviruses (RV-A) are the main etiologic agent of acute gastroenteritis in infants and young children worldwide (Estes and Kapikian, 2007) and an estimated 453,000 children aged <5 years die from rotavirus diarrhea each year, with >85% of these deaths occurring in low-income countries of Africa and Asia (Parashar et al., 2009;Tate et al., 2011). Rotaviruses belong to the family Reoviridae, and the rotavirus genome consists of 11 double-stranded RNA gene segments that encode six structural (VP) and six non-structural proteins (NSP). Based on the two genes that encode the outer capsid proteins, VP4 (P-type) and VP7 (G-type), a widely used binary classification system was established for RV-A (Estes and Kapikian, 2007). This system has been recently standardized and extended to all 11 genes (Matthijnssens et al., 2008b). To date, at least 27 G, 35 P, 16 I, 9 R, 9 C, 8 M, 16 A, 9 N, 12 T, 14 E and 11 H genotypes have been identified based on the eleven rotavirus A genes (Esona et al., 2010b;Matthijnssens et al., 2011). In humans, at least five RV-A G types (G1-G4 and G9), and two common P types (P[8] and P[4]) circulate worldwide (Banyai et al., 2012;Gentsch et al., 2005;Santos and Hoshino, 2005). G9 strains emerged in 1990s, and there has been a global description of the appearance and dominance of this genotype (Gentsch et al., 2005;Laird et al., 2003;Matthijnssens et al., 2009;Santos and Hoshino, 2005). Genotype G9 strains with a Wa-like or a DS-1-like genomic configuration or a mixture thereof have been detected sporadically in localized outbreaks (Page et al., 2010). In Cameroon, the first molecular identification of genotype G9 in human samples was reported in a study conducted by colleagues in 2003 (Steele andIvanoff, 2003). At least seven major phylogenetic lineages and eleven minor lineages within G9 VP7 genes have been described (Phan et al., 2007;Wu et al., 2011). A molecular evolutionary analysis study utilizing Bayesian inference supported the idea that one single sub-lineage introduced in the 1980s was responsible for all the worldwide spread of G9 in the 1990s .In order to gain insight into the degree of genetic variability of G9P [8] Viral RNA extraction, amplification, and sequencingViral RNA from each of the 15 specimens was extracted from a 10% stool suspension made from 0.1 g or 100 μl stool in 2 ml of a 1:1 Vertrel/Water solution using either a commercial RNA extraction kit (NucliSens automated extractor, BIOMERIEUX, Durham, NC) according to the protocol specified by the manufacturer or a silica binding method described pr...

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Section: Discussionmentioning

confidence: 74%

Section: Analysis Of Nsp4 Nucleotide Sequencesmentioning

confidence: 72%

Section: Analysis Of Nsp4 Nucleotide Sequencesmentioning

confidence: 99%

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Human G9P[8] rotavirus strains circulating in Cameroon, 1999–2000: Genetic relationships with other G9 strains and detection of a new G9 subtype

Esona

Mijatovic-Rustempasic

Foytich

et al. 2013

Infection, Genetics and Evolution

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“…Rotavirus acts on epithelial cells by altering protein trafficking, disrupting cell-cell interactions, and damaging tight junctions, thereby increasing paracellular permeability (Obert et al, 2000). The toxic rotavirus unstructured protein-4 induces age-and calcium ion-dependent chloride secretion and disrupts sodium-dependent glucose transporter-1 mediated reabsorption of water (Ball et al, 2005).…”

Section: Infectious Diarrhoeamentioning

confidence: 99%

The Role of Intestinal Barrier Function in Early Life in the Development of Colitis

Anderson¹,

Dalziel²,

Gopal³

et al. 2012

Colitis

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“…Moreover, the adjuavancity of NSP4 could increase the efficacy of ORF2 (11,12). In this study, the fusion of NSP4 to the truncated ORF2 was successfully assembled to VLP in BES.…”

Section: Discussionmentioning

confidence: 89%

A Study on the Virus-Like Particle Formation of Hepatitis E Virus ORF2 and Rotavirus NSP4 Protein in the Eukaryotic and Prokaryotic Expression Systems

Makvandi

Teimoori

Neisi

et al. 2017

Jundishapur J Microbiol

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Background: Hepatitis E virus (HEV) is able to induce fulminant hepatitis E infection in immunosuppressed individuals. Previous studies showed that the Baculovirus expression system (BES) could be used for developing hepatitis E virus-like particles (VLPs). Objectives: In the present study, the formation of VLPs of the recombinant proteins of HEV truncated open reading frame 2 (ORF2; 112 -607) and Rotavirus non-structural protein 4 (NSP4) (OSU-a) were investigated in BES and Escherichia. coli. Methods: To construct VLPs, the truncated ORF2-NSP4 protein was expressed in BES. The expression of protein was confirmed by western blot. This protein was expressed in E. coli. The truncated ORF2-NSP4 gene was subcloned in pET28a, and then transformed into E. coli DH5α. The confirmed colonies were transformed into E. coli BL21. The solubility of protein were checked by SDS-PAGE and western blot analysis. In the final step, to verify the VLP formation in BES and E. coli, the recombinant proteins were stained with 2% uranyl acetate and checked by transmission electron microscopy (TEM). Results:The 75 KDa truncated ORF2-NSP4 protein was successfully expressed in Sf9 cells, assembled, and formed VLP according to TEM results. In the prokaryotic expression system (E. coli), the ORF2-NSP4 gene was successfully subcloned and expressed in BL21 but VLP was not detected in TEM analysis. Conclusions:The truncated ORF2-NSP4 VLP were efficiently expressed in the SF9 cells, as a potential mucosal vaccine against HEV and Rotavirus. In the prokaryotic expression system (E. coli), the ORF2-NSP4 gene was successfully expressed.

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Rotavirus NSP4: A Multifunctional Viral Enterotoxin

Cited by 85 publications

References 128 publications

Human G9P[8] rotavirus strains circulating in Cameroon, 1999–2000: Genetic relationships with other G9 strains and detection of a new G9 subtype

Human G9P[8] rotavirus strains circulating in Cameroon, 1999–2000: Genetic relationships with other G9 strains and detection of a new G9 subtype

The Role of Intestinal Barrier Function in Early Life in the Development of Colitis

A Study on the Virus-Like Particle Formation of Hepatitis E Virus ORF2 and Rotavirus NSP4 Protein in the Eukaryotic and Prokaryotic Expression Systems

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