Rotavirus-like particles primary recovery from insect cells in aqueous two-phase systems☆

Benavides, Jorge; Mena, Jimmy A.; Cisneros-Ruiz, Mayra; Ramı́rez, Octavio T.; Palomares, Laura A.; Rito‐Palomares, Marco

doi:10.1016/j.jchromb.2006.05.006

Cited by 88 publications

(84 citation statements)

References 26 publications

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“…The molecular weight of the majority of the proteins in the 11S (glycinin) and 7S (β-conglycinin) fractionated extracts is around 360 KDa and 180 KDa, respectively. 13 -16 It has been reported 23,29 that proteins with high molecular weight generally concentrate in the bottom phase. An increase in system TLL had no effect on the partition behaviour of the proteins from the 11S fractionated soybean extract.…”

Section: Resultsmentioning

confidence: 99%

“…A long tie-line length usually affects protein solubility and causes proteins present on the top and bottom phases to accumulate at the interface. 23,31 The results obtained from the processing of fractionated soybean extracts in ATPS may suggest several recovery strategies for the potential purification of a recombinant protein. It is clear that ATPS can easily be implemented for the recovery of recombinant proteins expressed in soybean that exhibit top phase preference (e.g.…”

Section: Resultsmentioning

confidence: 99%

“…Potential processing of the interface of ATPS for the recovery of biological products has been addressed previously. 23 However, particular conditions to process the interface for the recovery of GUS from soybean proteins need to be established using conventional techniques such as precipitation or sequential ATPS extractions.…”

Section: Recovery Of Gus From Fractionated Soybean Extracts In Atpsmentioning

confidence: 99%

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Processing of soybean (Glycine max) extracts in aqueous two‐phase systems as a first step for the potential recovery of recombinant proteins

Aguilar

Rito‐Palomares

2007

J of Chemical Tech & Biotech

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BACKGROUND: The potential use of plants as production systems to establish bioprocesses has been established over the past decade. However, the lack of efficient initial concentration and separation procedures affect the generic acceptance of plants as economically viable systems. In this context the use of aqueous two-phase systems (ATPS) can provide strategies to facilitate the adoption of plants as a base for bioprocesses. Among the crops, soybeans (Glycine max) represent an attractive alternative since potentially they can produce high levels of recombinant protein. In this paper the processing of fractionated soybean extracts using ATPS is evaluated as a first step to recover recombinant proteins expressed in plants, using β-glucuronidase (GUS; E.C. 3.2.1.31) as a model protein.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Recovery Of Gus From Fractionated Soybean Extracts In Atpsmentioning

confidence: 99%

See 1 more Smart Citation

Processing of soybean (Glycine max) extracts in aqueous two‐phase systems as a first step for the potential recovery of recombinant proteins

Aguilar

Rito‐Palomares

2007

J of Chemical Tech & Biotech

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“…In this context, the recovery and purification of viruslike particles as delivery vectors for gene therapy represents an interesting case study. Benavides et al [17] reported the recovery and partial purification of double layered Rotavirus-Like Particles (dlRLP) produced by insect cells, i.e., baculovirus expression system using ATPS PEG-potassium phosphate. dlRLP have several applications, including vaccination against rotavirus, potential vectors in gene therapy, construction of functional nanoparticles, etc.…”

Section: Bio-nanoparticlesmentioning

confidence: 99%

“…These results demonstrated the great potential of ATPS for the recovery of these particles, particularly when compared with the yields achieved by commonly used virus-like particle recovery techniques such as cesium chloride and glucose gradients. In addition, the process developed using ATPS had advantages concerning total processing time, energy consumption and scaling up capability [17]. An area of increasing interest is the use of ATPS for the fractionation, assembly and recovery of bionanoparticles.…”

Section: Bio-nanoparticlesmentioning

confidence: 99%

Extraction and Purification of Bioproducts and Nanoparticles using Aqueous Two‐Phase Systems Strategies

et al. 2008

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Aqueous Two-Phase Systems (ATPS) is a primary recovery technique that has shown great potential for the efficient extraction and purification of high value biological compounds. The main advantages of this technique include scaling up feasibility, process integration capability and biocompatibility. In this review, the efficient use of ATPS for the extraction of proteins, genetic material, low molecular weight compounds, bioparticles, nanoparticles and cells is highlighted. The important role of ATPS in process integration, i.e., extractive conversion, extractive fermentation, cell disruption integrated with product recovery, and extractive purification, is discussed. A novel approach to protein molecular characterization combining ATPS and 2-dimension electrophoresis (2-DE) is introduced as a first step in the process development. Novel approaches for downstream processing using ATPS and dielectrophoresis are presented. Finally, trends concerning the application of ATPS strategies to address the future challenges of bioseparation are discussed.

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Aqueous Two‐Phase Systems (ATPSs) for Extraction and Purification of Biomolecules and Bioparticles

Marques

Molino

Mazzola

et al. 2013

Encyclopedia of Industrial Biotechnology

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The study of biomolecules and bioparticles has achieved great advances, thus increasing the productivity with upstream improvements. Nowadays, downstream processes are the bottleneck in the production of some biopharmaceuticals, a change from the previous years' scene. Current purification platforms will reach their physical limits at some point, creating the necessity for new approaches. This article reviews an alternative method for the extraction and purification of biomolecules/bioparticles denominated as aqueous two‐phase systems (ATPSs) . Biocompatibility and readiness to scale up are some of the characteristics of ATPSs and we also discuss its application in the biotechnology field.

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Rotavirus-like particles primary recovery from insect cells in aqueous two-phase systems☆

Cited by 88 publications

References 26 publications

Processing of soybean (Glycine max) extracts in aqueous two‐phase systems as a first step for the potential recovery of recombinant proteins

Processing of soybean (Glycine max) extracts in aqueous two‐phase systems as a first step for the potential recovery of recombinant proteins

Extraction and Purification of Bioproducts and Nanoparticles using Aqueous Two‐Phase Systems Strategies

Aqueous Two‐Phase Systems (ATPSs) for Extraction and Purification of Biomolecules and Bioparticles

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