Rotavirus Infection in Children with Diarrhea at Korle-Bu Teaching Hospital, Ghana

Damanka, Susan; Adiku, Theophilus; Armah, George; Rodrigues, Onike; Donkor, Eric S.; Nortey, David; Asmah, Richard Harry

doi:10.7883/yoken.jjid.2014.407

Cited by 22 publications

(26 citation statements)

References 26 publications

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“…The current study focused on the microbial pathogens associated with acute diarrhoea among children aged 5 years and below who visited some major hospitals within the Kumasi metropolis of Ghana. The current study suggested rota- and adenoviruses were the commonest diarrhoea causing pathogens emphasizing the important role of these pathogens in causing childhood diarrhoea and consistent with studies from Ghana and beyond [5, 11, 12]. Compared to control subjects, it was observed that being positive for any bacteria ( Salmonella spp.…”

Section: Discussionsupporting

confidence: 87%

Microbial pathogens associated with acute childhood diarrhoea in Kumasi, Ghana

et al. 2017

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BackgroundDiarrhoeal diseases are among the most frequent causes of morbidity and mortality in children worldwide, especially in sub-Saharan Africa. This case–control study was conducted to investigate the bacterial, viral and parasitic pathogens associated with acute diarrhoea among children attending three health facilities in Kumasi, Ghana.MethodsStool specimens were collected from 240 children under 5 years of age visiting hospitals in Kumasi, Ghana due to acute diarrhoea and from 107 healthy controls of similar age. Both intestinal and malaria parasites were diagnosed by microscopy whereas rota- and adenoviruses were identified by stool antigen immunochromatograhic testing. Bacterial enteropathogens were detected by conventional culture techniques.ResultsOf all subjects, 23 (6.6%) were positive for malaria parasitaemia, 139 (40.1%) had at least one bacterial agent in their stool and 25 (7.2%) had ova or parasites. Subjects infected with malaria had the highest odds of having diarrhoea [12.0 (95% CI 1.56, 92.35)] followed by those with rotaviruses [4.4 (95% CI 2.05, 9.47)] and bacterial infection [4.99 (95% CI 1.45, 17.17)].ConclusionIn conclusion, this study was unique as it looked at the three groups of pathogens (parasites, viruses and bacteria) that cause acute diarrhoea in children in the Kumasi metropolis of Ghana. This study has shown for the first time since 2004 that malaria parasitaemia, rotavirus and bacterial infections still remain common pathogens associated with acute childhood diarrhoea in the Kumasi metropolis of Ghana.

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Section: Discussionsupporting

confidence: 87%

Microbial pathogens associated with acute childhood diarrhoea in Kumasi, Ghana

et al. 2017

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“…Rotavirus gastroenteritis is highly contagious, and most children are infected with RVA before 5 years of age . The prevalence of rotavirus diarrhea in children varies among countries, from 6.5‐38% . While infections are observed throughout the year, cases predominate in the winter and spring in many countries …”

Section: Introductionmentioning

confidence: 99%

“…15 The prevalence of rotavirus diarrhea in children varies among countries, from 6.5-38%. [16][17][18][19] While infections are observed throughout the year, cases predominate in the winter and spring in many countries. 17,[20][21][22][23] In Turkey, acute gastroenteritis is a serious public health problem, affecting over 352 000 children younger than 5 years old annually (http://tsim.saglik.gov.tr/tsim).…”

Section: Introductionmentioning

confidence: 99%

Prevalence and diversity of rotavirus A genotypes cirulating in Turkey during a 2‐year sentinel surveillance period, 2014‐2016

Durmaz

Bakkaloğlu²,

Ünaldı³

et al. 2017

Journal of Medical Virology

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Human rotavirus A (RVA) is the main etiological agent of watery diarrhea among children under 5 years of age worldwide. The aims of this study were to investigate the prevalence and diversity of RVA genotypes circulating in Turkey during a 2-year sentinel surveillance study. A total of 1639 rotavirus antigen-positive stool samples were obtained from children younger than 5 years of age hospitalized with acute gastroenteritis. Rotavirus G and P genotypes were determined by reverse transcription polymerase chain reaction (RT-PCR) with consensus primers for the VP7 and VP4 genes, followed by semi-nested type-specific multiplex PCR. Rotavirus RNA was detected in 1396 (85.3%) of the samples tested. The highest detection rate (38.2%) was obtained among children in the 0-12 months age group, followed by children in the 13-24 months age group (36.2%). The most prevalent genotype was G1P[8] (24.6%) followed by G3P[8] (19.6%), G9P[8] (12.2%), G2P[4] (9.5%), G2P[8] (6.5%), and G4P[8] (4.8%). The proportions of uncommon and mixed genotypes were 21.5% and 1.14%, respectively. The large number of genotypes observed, including common, uncommon, and mixed types, indicates a high heterogeneity of RVA strains circulating in Turkey. The current study also exhibited dramatic fluctuations on the prevalences of the common genotypes, with increases in G3 and G1 and decreases in G9 and G2 from 2014-2016.

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“…14 In keeping with the findings from this study, other studies from Ghana have also reported genotypes P [4], P [6], and P [8], with the P [6] genotype being the most prevalent. 7,15 Strains with the VP7/VP4 genotypes: G3P [6], G12P [8], G1P [8], and G9P [4] were the most prevalent and accounted for 68% of the characterized strains, which differs somewhat from genotype prevalence reported previously-G2P [6], G3P [4] and G9P [8] as the most prevalent strains in northern Ghana, 16 and G1P [8], G3P [6], and G2P [6] as the most prevalent strains in southern Ghana. 14 The global emergence of the G12 strains in the postvaccine introduction era 17 probably explains the high incidence of the G12P [8] strain found in this study.…”

Section: Rva Prevaccine Introduction Era Surveillance Studies Havementioning

confidence: 67%

“…5 However, recent studies in developing countries have shown a greater diversity of RVA genotypes with several novel combinations. 6,7 The most effective measure of reducing the burden due to RVAs is by the use of vaccines. 8 Two licensed RVA vaccines (Rotarix and RotaTeq) have been recommended by the World Health Organization (WHO) for use globally.…”

Section: Introductionmentioning

confidence: 99%

Distribution of rotavirus genotypes in the postvaccine introduction era in Ashaiman, Greater Accra Region, Ghana, 2014‐2016

Letsa

Damanka

Dennis

et al. 2019

Journal of Medical Virology

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Group A Rotaviruses (RVAs) are the most important etiological agents of acute gastroenteritis (AGE) in children less than 5 years of age. Mortality resulting from RVA gastroenteritis is higher in developing countries than in developed ones, causing a huge public health burden in global regions like Africa and South‐East Asia. This study reports RVA genotypes detected in Ashaiman, Greater Accra Region, Ghana, in the postvaccine introduction era for the period 2014‐2016. Stool samples were collected from children less than 5 years of age who visited Ashaiman Polyclinic with AGE from November 2014 to May 2015 and from December 2015 to June 2016. The samples were tested by enzyme immunoassay (EIA), and one‐step multiplex reverse transcription polymerase chain reaction was performed on the EIA positive samples for gel‐based binomial genotyping. Of the 369 stool samples collected from children with AGE, 145 (39%) tested positive by EIA. Five VP7 (G1, G3, G9, G10, and G12) and three VP4 (P[4], P[6] and P[8]) genotypes were detected. Eight G/P combinations were identified of which, G3P[6], G12P[8], G1P[8], and G9P[4] were the most prevalent and responsible for 93 (68%) of the AGE cases, and seven mixed‐types were detected which represented 8% of the RVA cases. High prevalence, diversity, and mixed‐types of RVAs were detected from Ashaiman with the emergence of unusual genotypes.

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Rotavirus Infection in Children with Diarrhea at Korle-Bu Teaching Hospital, Ghana

Cited by 22 publications

References 26 publications

Microbial pathogens associated with acute childhood diarrhoea in Kumasi, Ghana

Microbial pathogens associated with acute childhood diarrhoea in Kumasi, Ghana

Prevalence and diversity of rotavirus A genotypes cirulating in Turkey during a 2‐year sentinel surveillance period, 2014‐2016

Distribution of rotavirus genotypes in the postvaccine introduction era in Ashaiman, Greater Accra Region, Ghana, 2014‐2016

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