Rotavirus-induced miR-142-5p elicits proviral milieu by targeting non-canonical transforming growth factor beta signalling and apoptosis in cells

Chanda, Shampa; Nandi, Satabdi; Chawla‐Sarkar, Mamta

doi:10.1111/cmi.12544

Cited by 39 publications

(40 citation statements)

References 54 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…A latest research reported that up-regulated miR-142-5p inhibits TGF-β-induced apoptosis via targeting TGFBR2 and SMAD3 in rotavirus infection model [33]. Our studies indicated that miR-142-5p overexpression significantly downregulated SMAD3 to diminish TGF-β-induced growth arrest, supporting Chanda et al research from several tumor cell lines.…”

Section: Discussionsupporting

confidence: 87%

“…We analyzed the upstream sequence of the miR-142 gene and also found a MYC-binding site in its promoter, suggesting that MYC regulates the biosynthesis of miR-142 precursors. Collectively, our and other studies indicated that miR-142-5p/3p attenuate TGF-β signal transduction by targeting TGFBRI/II and SMAD3 [33, 41]. A combinatorial miRNA-TF feedback loop could be constructed to modulate the TGF-β signaling.…”

Section: Discussionmentioning

confidence: 62%

See 1 more Smart Citation

MicroRNA regulatory pathway analysis identifies miR-142-5p as a negative regulator of TGF-β pathway via targeting SMAD3

Huang

et al. 2016

Oncotarget

View full text Add to dashboard Cite

MicroRNAs (miRNAs) are non-coding RNAs with functions of posttranscriptional regulation. The abnormally expressed miRNAs have been shown to be crucial contributors and may serve as biomarkers in many diseases. However, determining the biological function of miRNAs is an ongoing challenge. By combining miRNA targets prediction, miRNA and mRNA expression profiles in TCGA cancers, and pathway data, we performed a miRNA-pathway regulation inference by Fisher's exact test for enrichment analysis. Then we constructed a database to show the cancer related miRNA-pathway regulatory network (http://bioinfo.life.hust.edu.cn/miR_path). As one of the miRNAs targeting many cancer related pathways, miR-142-5p potentially regulates the maximum number of genes in TGF-β signaling pathway. We experimentally confirmed that miR-142-5p directly targeted and suppressed SMAD3, a key component in TGF-β signaling. Ectopic overexpression of miR-142-5p significantly promoted tumor cell proliferation and inhibited apoptosis, while silencing of miR-142-5p inhibited the tumor cell proliferation and promoted apoptosis in vitro. These findings indicate that miR-142-5p plays as a negative regulator in TGF-β pathway by targeting SMAD3 and suppresses TGF-β-induced growth inhibition in cancer cells. Our study proved the feasibility of miRNA regulatory pathway analysis and shed light on combining bioinformatics with experiments in the research of complex diseases.

show abstract

Section: Discussionsupporting

confidence: 87%

Section: Discussionmentioning

confidence: 62%

MicroRNA regulatory pathway analysis identifies miR-142-5p as a negative regulator of TGF-β pathway via targeting SMAD3

Huang

et al. 2016

Oncotarget

View full text Add to dashboard Cite

show abstract

“…Some of the miRNA that were altered in the circulation e.g. miRs 142-5p, 150-5p and 21-5p have been described in anti-apoptotic responses and may signify cellular compensation for increased hematopoietic death induced by radiation21222324.…”

Section: Discussionmentioning

confidence: 99%

Changes in miRNA in the lung and whole blood after whole thorax irradiation in rats

Gao

Liu

Narayanan

et al. 2017

Sci Rep

View full text Add to dashboard Cite

We used a rat model of whole thorax x-ray irradiation to profile the microRNA (miRNA) in lung and blood up to 4 weeks after radiation. MiRNA from normal and irradiated Wistar rat lungs and whole blood were analyzed by next-generation sequencing and the changes by radiation were identified by differential deRNA-seq 1, 2, 3 and 4 weeks after irradiation. The average total reads/library was 2,703,137 with a mean of 88% mapping to the rat genome. Detailed profiles of 100 of the most abundant miRNA in rat blood and lung are described. We identified upregulation of 4 miRNA, miR-144-5p, miR-144-3p, miR-142-5p and miR-19a-3p in rat blood 2 weeks after radiation that have not previously been shown to be altered after radiation to the lung. Ingenuity Pathway Analysis identified signaling of inflammatory response pathways. These findings will support development of early detection methods, as well as mechanism(s) of injury and mitigation in patients after radiotherapy or radiological accidents.

show abstract

“…84) or IL-22 and IFN 85 , have been proposed to mediate the clearance of rotaviruses by the innate immune system in mice 77 . In addition, rotavirus infection of human Caco-2 cells in vitro 86,87 and in piglets 88 induces transforming growth factor-β (TGFβ) expression, which can downregulate the adaptive immune response to rotavirus infection (see Recurrent infections). In general, rotaviruses have several features that enable them to efficiently evade the innate immune response, leaving the adaptive immune response as a final mechanism for viral clearance.…”

Section: Mechanisms/pathophysiologymentioning

confidence: 99%

Rotavirus infection

Crawford

Ramani

Tate

et al. 2017

Nat Rev Dis Primers

526

558

View full text Add to dashboard Cite

Rotavirus infections are a leading cause of severe, dehydrating gastroenteritis in children <5 years of age. Despite the global introduction of vaccinations for rotavirus over a decade ago, rotavirus infections still result in >200,000 deaths annually, mostly in low-income countries. Rotavirus primarily infects enterocytes and induces diarrhoea through the destruction of absorptive enterocytes (leading to malabsorption), intestinal secretion stimulated by rotavirus non-structural protein 4 and activation of the enteric nervous system. In addition, rotavirus infections can lead to antigenaemia (which is associated with more severe manifestations of acute gastroenteritis) and viraemia, and rotavirus can replicate in systemic sites, although this is limited. Reinfections with rotavirus are common throughout life, although the disease severity is reduced with repeat infections. The immune correlates of protection against rotavirus reinfection and recovery from infection are poorly understood, although rotavirus-specific immunoglobulin A has a role in both aspects. The management of rotavirus infection focuses on the prevention and treatment of dehydration, although the use of antiviral and anti-emetic drugs can be indicated in some cases.

show abstract

Rotavirus-induced miR-142-5p elicits proviral milieu by targeting non-canonical transforming growth factor beta signalling and apoptosis in cells

Cited by 39 publications

References 54 publications

MicroRNA regulatory pathway analysis identifies miR-142-5p as a negative regulator of TGF-β pathway via targeting SMAD3

MicroRNA regulatory pathway analysis identifies miR-142-5p as a negative regulator of TGF-β pathway via targeting SMAD3

Changes in miRNA in the lung and whole blood after whole thorax irradiation in rats

Rotavirus infection

Contact Info

Product

Resources

About