Rotavirus disease burden among children &lt;5 years of age – Santa Rosa, Guatemala, 2007–2009

Arvelo, Wences; López, Beatriz; Reyes, Lissette; Kerin, Tara; Gautam, Rashi; Patel, Manish; Parashar, Umesh D.; Lindblade, Kim A.

doi:10.1111/j.1365-3156.2011.02911.x

Cited by 16 publications

(16 citation statements)

References 12 publications

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“…Rotavirus is the agent most commonly causing diarrhea in children between six months and two years of age [8, 9], with incidence and severity diminishing after age five [10, 11]. Our study was consistent with the epidemiological studies, those in the literature in that most of the patients were younger than two years old (with the mean of 20.9 months).…”

Section: Discussionsupporting

confidence: 89%

The Comparition of the Efficacy of Two Different Probiotics in Rotavirus Gastroenteritis in Children

Erdoğan

Tanyeri

Torun

et al. 2012

Journal of Tropical Medicine

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Objectives. The aim of the study is to compare the clinical effectiveness of the probiotics—Saccharomyces boulardii and Bifidobacterium lactis—in children who had been diagnosed with rotavirus gastroenteritis. Materials and methods. Seventy five patients aged between 5 months–5 years diagnosed as rotavirus gastroenteritis were included in the study. The patients diagnosed as rotavirus gastroenteritis by latex agglutination test in stool were divided into 3 groups of twenty-five patients each: First group was given oral rehydration therapy and rapid refeeding with a normal diet with Saccharomyces boulardii (spp. I-745), second group was given oral rehydration therapy and rapid refeeding with a normal diet with Bifidobacterium lactis (spp. B94, culture number:N°118529) and third group received only oral rehydration therapy and rapid refeeding with a normal diet. Results. The duration of diarrhea was shorter in the group given oral rehydration therapy and rapid refeeding with a normal diet with Bifidobacterium lactis and Saccharomyces boulardii than the group given only oral rehydration therapy and rapid refeeding with a normal diet. Conclusion. Bifidobacterium lactis has a complemental role in the treatment of rotavirus gatroenteritis and other probiotics may also have a beneficial effect in rotavirus gastroenteritis compared with the therapy included only oral rehydration therapy and rapid refeeding with a normal diet.

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Section: Discussionsupporting

confidence: 89%

The Comparition of the Efficacy of Two Different Probiotics in Rotavirus Gastroenteritis in Children

Erdoğan

Tanyeri

Torun

et al. 2012

Journal of Tropical Medicine

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“…Stool samples (n = 91) from surveillance studies and outbreak investigations in the USA and international surveillance studies (Cardemil et al, 2012; Cortes et al, 2012; Gentsch et al, 2009; Hull et al, 2011) were tested in this study. All samples were de-identified and could not be traced back to patient or hospital case identifiers.…”

Section: Methodsmentioning

confidence: 99%

Sensitive and specific nested PCR assay for detection of rotavirus A in samples with a low viral load

Mijatovic-Rustempasic

Esona

Williams

et al. 2016

Journal of Virological Methods

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Techniques such as the real-time reverse transcription-polymerase chain reaction (qRT-PCR) can detect RNA in samples with a low viral load. However, these amplicons typically are either too short or at insufficient concentrations for use in subsequent sequencing reactions for genotyping and detection confirmation. The assay developed in this study detects rotavirus G genotypes and P genotypes with viral loads as low as 6.2 and 8.2 copies per reaction, respectively. The assay was validated using a panel of 91 stool samples, 32 reference rotavirus strains, and 6 non-target enteric virus samples.

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“…This time period was selected as it corresponds to the rotavirus season based on laboratory-based surveillance data [9]. Both specimen types were collected simultaneously and within 24 h of admission to the hospital or during the ambulatory clinic visit.…”

Section: Methodsmentioning

confidence: 99%

Diagnostic performance of rectal swab versus bulk stool specimens for the detection of rotavirus and norovirus: Implications for outbreak investigations

Arvelo

Hall

Estévez

et al. 2013

Journal of Clinical Virology

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Background In January of 2008, during the peak of the rotavirus season in Guatemala, a gastroenteritis outbreak with high mortality among infants was reported in Guatemala. Despite extensive efforts, the investigation was limited by the lack of bulk stool specimens collected, particularly from the more severely dehydrated or deceased children. Objectives We evaluated the diagnostic performance of rectal swab specimens compared with bulk stool for the detection of rotavirus and norovirus. Study design Patients with diarrhea (≥3 loose stools in 24 h) were enrolled through an ongoing surveillance system in Guatemala. From January through March 2009, we attempted to enroll 100 patients <5years old captured by the diarrhea surveillance, and collected paired bulk stool and rectal swabs specimens from them. Specimens were tested for norovirus using real-time reverse transcription-polymerase chain reaction and for rotavirus via enzyme immunoassay. Results We enrolled 102 patients with paired specimens; 91% of 100 paired specimens tested for rotavirus yielded concordant results positive for rotavirus with a negativity rate of 83%. Among 100 paired specimens tested for norovirus, 86% were concordant norovirus detection and the negativity rate was 85%. The diagnostic performance for rotavirus and norovirus detection did not differ significantly between the two specimen types. Conclusions Testing of properly collected fecal specimens using rectal swabs may be a viable alternative to bulk stool for detection of rotavirus and norovirus, particularly during outbreaks where collection of bulk stool may be difficult.

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Rotavirus disease burden among children <5 years of age – Santa Rosa, Guatemala, 2007–2009

Cited by 16 publications

References 12 publications

The Comparition of the Efficacy of Two Different Probiotics in Rotavirus Gastroenteritis in Children

The Comparition of the Efficacy of Two Different Probiotics in Rotavirus Gastroenteritis in Children

Sensitive and specific nested PCR assay for detection of rotavirus A in samples with a low viral load

Diagnostic performance of rectal swab versus bulk stool specimens for the detection of rotavirus and norovirus: Implications for outbreak investigations

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