Rotation of fluorescent probes localized within lipid bilayer membranes

Jacobson, Kenneth A.; Wobschall, Darold

doi:10.1016/0009-3084(74)90049-8

Cited by 42 publications

(19 citation statements)

References 33 publications

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“…Perylene was obtained from Molecular Probes (Eugene, OR). The concentration of perylene stock solution (in chloroform) was determined using an extinction coefficient at 410 nm equal to 35,000 M Ϫ1 cm Ϫ1 (in ethanol) (Jacobson and Wobschall, 1974). The concentration of phospholipids was determined according to the method of Bartlett (1959).…”

Section: Methodsmentioning

confidence: 99%

Studies of Archaebacterial Bipolar Tetraether Liposomes by Perylene Fluorescence

Khan

Chong

2000

Biophysical Journal

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Membrane packing and dynamics of bipolar tetraether liposomes composed of the polar lipid fraction E (PLFE) from the thermoacidophilic archaebacterium Sulfolobus acidocaldarius have been studied by perylene fluorescence. At a probe-to-PLFE lipid ratio of 1:400, we have detected an unusual fluorescence intensity increase with increasing temperature, while the fluorescence lifetime changed little. As the ratio was decreased, the intensity anomaly was diminished. At 1:3200 and 1:6400, the anomaly disappeared. A remarkable perylene intensity anomaly was also observed in bilayers composed of saturated monopolar diester phosphatidylcholines at their main phase transition temperatures. These results suggest that the intensity anomaly may be due to probe aggregation caused by tight membrane packing. At the same probe-to-lipid ratio (1:400), however, 1, 2-diphytanoyl-sn-glycero-3-phosphocholine (DPhPC) and 1, 2-diphytanoyl-sn-glycero-3-phosphoglycerol (DPhPG) liposomes did not exhibit any intensity anomaly with increasing temperature. This suggests that DPhPC and DPhPG liposomes are more loosely packed than PLFE liposomes; thus the branched methyl groups are not the contributing factor of the tight membrane packing found in PLFE liposomes. Using a multiexcitation method, we have also determined the average (R), in-plane (R(ip)), and out-of-plane (R(op)) rotational rates of perylene in PLFE liposomes at various temperatures (20-65 degrees C). R and R(ip), determined at two different probe-to-lipid ratios (1:400 and 1:3200), both undergo an abrupt increase when the temperature is elevated to approximately 48 degrees C. These data suggest that PLFE liposomes are rigid and tightly packed at low temperatures, but they begin to possess appreciable "membrane fluidity" at temperatures close to the minimum growth temperature ( approximately 50 degrees C) of thermoacidophilic archaebacteria.

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Section: Methodsmentioning

confidence: 99%

Studies of Archaebacterial Bipolar Tetraether Liposomes by Perylene Fluorescence

Khan

Chong

2000

Biophysical Journal

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“…As shown in Table 2, membranes for which this correction is significant incorporate cholesterol. For example, Cogan et al [10] observed an order of magnitude increase in microviscosity for the mixed lipid, egg-PC-CHL, compared to the phospholipid alone, while for the same system Jacobsen and Wobschall [22] report a factor of three increase in microviscosity. Szabo [37] observed a factor of five decrease in the mobility of the trinactin-NH 2 complex in GMO-CHL(I:I) membranes in comparison with GMO membranes.…”

Section: Discussionmentioning

confidence: 96%

Effect of ionic polarizability on electrodiffusion in lipid bilayer membranes

Bradshaw

Robertson

1975

J. Membrain Biol.

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Ion-carrier complexes and organic ions of similar size and shape have mobilities in lipid bilayer membranes which span several orders of magnitude. In this communication, an examination is made of the hypothesis that the basis for this unusually wide range of ionic mobilities is the potential energy barrier arising from image forces which selectively act on ions according to their polarizability. Using Poisson's equation to evaluate the electrostatic interaction between an ion and its surroundings, the potential energy barrier to ion transport due to image effects is computed, with the result that the potential energy barrier height depends strongly on ionic polarizability. Theoretical membrane potential energy profile calculations are used in conjunction with Nernst-Planck electrodiffusion equation to analyze the available mobility data for several ion-carrier complexes and lipid-soluble ions in lipid bilayer membranes. The variation among the mobilities of different ions is shown to be in agreement with theoretical predictions based on ionic polarizability and size. Furthermore, the important influence exerted by image forces on ion transport in lipid bilayer membranes compared to the frictional effect of membrane viscosity is established by contrasting available data on the activation energy of ionic conductivity with that for membrane fluidity.

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“…Penetration of foreign molecules into lipid bilayers also seems to be increased near a phase transition point, äs high lateral compressibility of the monolayer is associated with the onset of a gel-like transition. In turn, large density fluctüations and enhanced compressibility probably occur in the vicinity of a phase transition point, so dye binding should be altered in any disturbed lipid region (26)(27)(28).…”

Section: Discussionmentioning

confidence: 99%

1-Anilino-8-naphthalenesulphonate Binding Parameters in Red Cell Membranes. Does Diabetes Mellitus Affect Cell Membrane Dynamics?

Watała

Jóźwiak²

1989

Clinical Chemistry and Laboratory Medicine

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Summary:In this study we report the use of l-anilino-8-naphthalenesulphonate äs a fluorescence probe to investigate the properties of plasma membranes derived from normal and diabetic red blood cells.The binding of l-anilino-8-naphthalenesulphonate to diabetes-affected erythrocyte membranes, äs compared with controls, was measured by means of fluorescence polarization and fluorescence titration techniques.These measurements demonstrated anomalous l-anilino-^S-naphthalenesulphonate binding to pathological red cell membranes. The amount of l-anilino-8-naphthalenesulphonate bound to diabetic erythrocyte membranes was greater than that of controls. This fluorometric study indicated that the outer monolayer binding of l-anilino-8-naphthalenesulphonate was markedly augmented in the erythrocyte membranes of diabetic patients. Significant correlations were found between l-anilino-8-naphthalenesulphonate binding parameters and membrane lipid composition. The correlation between these binding parameters and non-enzymatic protein glycation was poor or moderate.Though the fluorescence intensity and emission maximum were quite similar in both groups investigated, binding studies reveäled that there were approximately 1.2 times the number of l-anilino-8-naphthalenesulphonate binding sites and a 33% increase in the K O value in diabetic membranes, suggesting significant differences in the environment of the l-anilino-8-naphthalenesulphonate binding sites in these two groups of patients.The results presented in this report indicate that (a) l-aniliüo-8-naphthalenesulphonate is a sensitive probe of membrane architecture alterations, and can be used to elucidate the perturbating effects of sterols in membrane Systems, and (b) that significant differences in membrane dynamics exist between normal and diabetic red cell membranes.The possibility that the increase in bound dye in diabetics was caused by enhanced lateral compressibility, or deiisity flüctuations, of whether it was due to additional binding sites at the boundary of heterogeneous lipid chisters is discussed. troductiono f fluorescence spectroscopy has been The revival of interest in fluorescence probes in recent used to probe many parameters of membrane strucyears has resulted in numerous studies dealing with ture using a variety of extrinsic probe molecules (2, thepertinehcepfselectedflüorophoresformonitoring 3), and several cheinicals have been especially dechanges of membrane structure and dynamics (l, 2), signed for this purpose. Amongst them, l-anilino-8-

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Rotation of fluorescent probes localized within lipid bilayer membranes

Cited by 42 publications

References 33 publications

Studies of Archaebacterial Bipolar Tetraether Liposomes by Perylene Fluorescence

Studies of Archaebacterial Bipolar Tetraether Liposomes by Perylene Fluorescence

Effect of ionic polarizability on electrodiffusion in lipid bilayer membranes

1-Anilino-8-naphthalenesulphonate Binding Parameters in Red Cell Membranes. Does Diabetes Mellitus Affect Cell Membrane Dynamics?

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