Rosuvastatin protects tissue perfusion in the experimental testicular torsion model

Abstract

Recently, anti-inflammatory and tissue protective effects of statins have been shown independent from its anti-hyperlipidemic effect. It has been shown that one of the statins, rosuvastatin, may reduce ischemia/reperfusion (I/R)-induced tissue injury in the brain, intestines, and heart. We planned an experimental study to evaluate the effect of rosuvastatin on I/R injury encountered after the detorsion of the testicular torsion. Rats were divided into three groups. In group 1, testis basal blood flow (basal value) was measured with LASER Doppler flowmeter (LDF). Testis was relocated into the scrotum without torsion. Two and 3 h after the basal measurement, testis was brought out from the same incision, and the second (second value) and third (third value) testicular blood flow measurements were done, respectively. In group 2, after the measurement of basal value testicular torsion was created. Second and third value measurements were obtained with LDF at the end of the 2 h of testicular torsion just before the detorsion and 1 h after detorsion. In group 3, same procedures in torsion/detorsion group were repeated in this group, but 10 mg/kg rosuvastatin was injected intraperitoneally 30 min before detorsion. Second values in groups 2 and 3 were significantly lower than group 1. Third values were significantly low in group 2 compared to groups 1 and 3. Regarding the third measurement, there was no significant difference between the groups 1 and 3. Tissue injury is closely related with condition of microvascular perfusion after I/R. Rosuvastatin can protect tissue perfusion in the experimental testicular torsion model.

Effects of adipose derived mesenchymal stem cells and erythropoietin in testicular torsion-induced the germ cell injury in the adult albino rat

“…Previous researchers (Yurtc et al 2009) observed a loosening of cell-cell contacts in the testicular I/R and attributed these separations to shrinkage of both germ and Sertoli cells. Former studies (Karakaya et al 2010) reported that there was disorganization of germ cell structure and affection of their function. They explained disorganization by elevated level of reactive oxygen species (ROS).…”

L-Alanyl-Glutamine dipeptide pretreatment attenuates ischemia-reperfusion injury in rat testis

“…To date, a number of chemicals and drugs, such as oxygen radical scavengers, have been successfully used to reduce I/R injuries in animal models of testicular torsion, but few of these substances are currently in clinical use 14 . Karakaya et al 15 evaluated the effects of Rosuvastatin, a synthetic statin, in rats torted testes (2 hours ischemia / reperfusion 1 hour) and concluded that Rosuvastatin can protect tissue perfusion in the experimental testicular torsion model. Erol et al 16 investigated the effect of intraperitoneal vardenafil (1 mg/kg) administration during an ischemic period in a rat model of testicular torsion/detorsion (1 hour ischemia/4 hours reperfusion) and concluded that vardenafil reduced MDA levels and decreased ischemia/reperfusion cellular damage.…”

Contemporary review of testicular torsion: New concepts, emerging technologies and potential therapeutics

“…The rosuvastatin group had return of blood flow following detorsion equal to the control group (sham operation) and significantly better than the torsion group. [59] Interpretation of these results indicates that rosuvastatin may preserve or salvage tissue perfusion in an experimental testicular torsion animal model.…”

“…The theory is that these drugs can lead to improvement of the testicular function (particularly germ cell function) following an episode of torsion when the testis is salvaged. Although not tested in human testicular torsion, drugs such as vardenafil [57], sildenafil [58], rosuvastatin [59] and poly(adenosine diphosphate-ribose) polymerase (PARP) inhibitors [60, 61], and supplements such as coenzyme Q10 [62], lycopene [63] and ginkgo biloba [64] have been studied in rat models of testicular torsion.…”