Rosuvastatin promotes angiogenesis and reverses isoproterenol-induced acute myocardial infarction in rats: Role of iNOS and VEGF

Zaitone, Sawsan A.; Abogresha, Noha M.

doi:10.1016/j.ejphar.2012.06.022

Cited by 75 publications

(53 citation statements)

References 51 publications

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“…The results of the majority of studies suggest that strengthening the antioxidant defenses may be one of the strongest proposed therapeutic candidates against cardiac remodeling (17).…”

Section: Discussionmentioning

confidence: 99%

“…It is well-established that angiogenesis plays a critical role in various pathological settings, including tumor growth, wound repair and MI, and may promote cardiac growth and maintain contractile function (17). Several angiogenic factors, including vascular endothelial growth factor (VEGF), hypoxia-inducible factor-1a (HIF-1a), eNOS and others are involved in this process (5,17).…”

Section: Discussionmentioning

confidence: 99%

“…Several angiogenic factors, including vascular endothelial growth factor (VEGF), hypoxia-inducible factor-1a (HIF-1a), eNOS and others are involved in this process (5,17).…”

Section: Discussionmentioning

confidence: 99%

“…To induce experimental heart failure, after 8 weeks of study, ISO was dissolved in normal saline and injected subcutaneously to rats (130 mg/kg) daily for two consecutive days (17).…”

Section: Methodsmentioning

confidence: 99%

See 3 more Smart Citations

Regular Exercise or Food Restriction, Which is Better in the Event of Heart Failure? An Approach to Oxidative Stress and Angiogenesis

Moradi

Imani

Shakoori

et al. 2016

Ann. Appl. Sport Sci

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The aim of present study was to investigate whether food restriction combined with exercise training could attenuate the oxidative stress and promote angiogenesis in a rat model of heart failure. 50 male wistar rats weighing 250-300 g were randomly divided into 5 groups including: 1) sham; they were fed ad libitum food, n=10. 2) Heart failure group; 3) Exercise group; they run on a treadmill 5 days per week for 4 weeks, n=10. 4) Food restricted group; they were fed with 60% of their daily average food intake, n=10. 5) Food restricted plus exercise group; as well as feeding with 60% of their daily average food intake for 8 weeks and run on a treadmill 5 days for the 4 next weeks, n=10. Subcutaneous injection of isoproterenol (130 mg/kg) was used to induce experimental heart failure. Echocardiographic parameters were monitored. Plasma levels of malondialdehyde (MDA) and prooxidant/ antioxidant balance (PAB), as oxidative parameters were measured. In continue gene expression of angiogenic factors such as hypoxia inducible factor-1a (HIF-1a), vascular endothelial growth factor A (VEGFA) and endothelial nitric oxide synthase (eNOS) as well as histopathological examination were investigated. Isoproterenol-treated hearts showed lower functional indexes including LVEDd; Left Ventricular End Diastolic dimension (p<0.05), FS; Fractional Shortening (p<0.001), EF; Ejection Fraction, (p<0.001). In addition, significant increase in plasma levels of MDA (p<0.001) and PAB (p<0.001) were observed. Food restriction and exercise significantly improved all measured parameters. The protective role of food restriction and exercise training on myocardial damage was further confirmed by promoting the gene expression of angiogenic factors (p<0.001) in left ventricle and reducing the myocardial fibrosis (p<0.05). Our results suggest that combined food restriction with exercise training is superior to either therapy alone for improving functional indexes, strengthen balance of antioxidative defense system, as well as gene expression of angiogenic factors and decreasing myocardial fibrosis.

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“…The results of the majority of studies suggest that strengthening the antioxidant defenses may be one of the strongest proposed therapeutic candidates against cardiac remodeling (17).…”

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

“…Several angiogenic factors, including vascular endothelial growth factor (VEGF), hypoxia-inducible factor-1a (HIF-1a), eNOS and others are involved in this process (5,17).…”

Section: Discussionmentioning

confidence: 99%

“…To induce experimental heart failure, after 8 weeks of study, ISO was dissolved in normal saline and injected subcutaneously to rats (130 mg/kg) daily for two consecutive days (17).…”

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Regular Exercise or Food Restriction, Which is Better in the Event of Heart Failure? An Approach to Oxidative Stress and Angiogenesis

Moradi

Imani

Shakoori

et al. 2016

Ann. Appl. Sport Sci

View full text Add to dashboard Cite

show abstract

“…In addition to having a proliferative effect, statins have also been shown to exert positive effects on cell survival and differentiation by reducing apoptosis and suppressing apoptotic cytokines. 15,29 Rosuvastatin facilitated local release of endothelium-related cytokines in aneurysm necks, which may promote endothelialization. In addition, the paracrine effect of EPCs may contribute to the acceleration of endothelialization.…”

Section: Discussionmentioning

confidence: 99%

Rosuvastatin for enhancement of aneurysm neck endothelialization after coil embolization: promotion of endothelial progenitor cells in a rodent model

Liu

Chen

et al. 2016

JNS

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OBJECT Coil embolization is a safe, efficient, and minimally invasive technique for the treatment of intracranial aneurysms. However, coil embolization is associated with a higher risk of recurrence than clip ligation. In this study, the authors explore a new approach through the promotion of endothelial progenitor cells (EPCs) to optimize endothelialization of the aneurysm neck and reduce the risk of recurrence. METHODS A coiled aneurysm model was created in 48 adult male Sprague-Dawley rats via microsurgery. Half of these animals were treated with rosuvastatin (20 mg/kg) in saline via gavage for 10, 20, or 30 days. The other half were administered saline without rosuvastatin. An additional 15 rats underwent “mock surgery” (identical anesthesia and saline gavage but no surgery). The endothelial repair process in the coiled aneurysms was evaluated via flow cytometry, im-munostaining, and electronic microscopy. The mock surgery group was used for comparison in flow cytometry studies. The effects of rosuvastatin on viability and functioning of Sprague-Dawley rat bone marrow-derived EPCs were also explored via MTT, migration, and tube formation assays. RESULTS The aneurysm neck repair score was significantly higher in the rosuvastatin-treated rats than in the untreated rats (p < 0.05). The circulating EPC count was increased and maintained at a higher level in rosuvastatin-treated rats compared with the aneurysm rats that did not receive rosuvastatin (p < 0.05). Immunostaining showed that the aneurysm neck endothelium was more integrated and the number of kinase insert domain receptor-positive cells was increased in the rosuvastatin-treated rats. Further study demonstrated that rosuvastatin promoted EPC proliferation, migration, and tube formation. CONCLUSIONS Rosuvastatin promoted endothelialization of the coiled aneurysm neck via induction of EPCs, suggesting that promoting endothelialization provides an additional therapeutic opportunity during vascular endothelium repair.

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Initial research on the effect and mechanism of Tivozanib on pulsed dye laser induced angiogenesis

et al. 2022

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Introduction: Pulsed dye laser (PDL) is the main treatment for port wine stain (PWS), but a considerable number of patients show low clearances. The reason for the poor efficacy is related to PDL-induced angiogenesis. Vascular endothelial growth factor (VEGF) plays an important role in PDL-induced angiogenesis and can activate the tyrosine kinase activity of VEGF receptor (VEGFR) in endothelial cells. It triggers a full range of responses, and then participates in the regulation of angiogenesis. Tivozanib is an inhibitor of VEGFR tyrosine kinase activity, which can block the pro-angiogenic effect of VEGF and reduce vascular permeability. Method: Different energy densities of PDL were used to irradiate the abdominal skin of rats. According to the general and pathological changes of the irradiated area, the energy density of 8 J/cm 2 with smaller scab and stronger vascular effect was selected for follow-up experiments. Divided the rat abdomen skin into four areas, irradiated three of them uniformly with an energy density of 8 J/cm 2 , and applied different concentrations of Tivozanib coating agent to the laser irradiation area, and grouped them as follows: (1) vacant group, (2) control group, (3) 0.5% Tivozanib group, (4) 1% Tivozanib group. Camera and dermoscopy were used to observe skin changes. Hematoxylin-eosin staining, immunohistochemical staining, and blood vessels were counted to detect dermal vascular regeneration. Transcriptome sequencing and real-time polymerase chain reaction (PCR) were conducted to elucidate the mechanism and validate the reliability.Results: The number of blood vessels in the 0.5% Tivozanib group and 1% Tivozanib group was significantly reduced on the 7, 10, and 14 days compared with the control group. The number of blood vessels in the 1% Tivozanib group was significantly reduced compared with the 0.5% Tivozanib group, indicating that Tivozanib successfully inhibited PDL-induced angiogenesis, and the inhibitory effect of 1% Tivozanib was more significant than that of 0.5% Tivozanib. Transcriptome sequencing results showed a total of 588 significantly differentially expressed genes, including 90 upregulated genes and 498 downregulated genes. Gene ontology (GO) and kyoto encyclopedia of genes and genomes (KEGG) enrichment analysis showed that the significantly differentially expressed genes were mainly enriched in the metabolic pathways which were closely related to angiogenesis. Finally, real-time PCR was used to verify the genes with higher expression differences, the top ranking and closely related to angiogenesis, namely,

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Rosuvastatin promotes angiogenesis and reverses isoproterenol-induced acute myocardial infarction in rats: Role of iNOS and VEGF

Cited by 75 publications

References 51 publications

Regular Exercise or Food Restriction, Which is Better in the Event of Heart Failure? An Approach to Oxidative Stress and Angiogenesis

Regular Exercise or Food Restriction, Which is Better in the Event of Heart Failure? An Approach to Oxidative Stress and Angiogenesis

Rosuvastatin for enhancement of aneurysm neck endothelialization after coil embolization: promotion of endothelial progenitor cells in a rodent model

Initial research on the effect and mechanism of Tivozanib on pulsed dye laser induced angiogenesis

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