Ross operation with a tissue-engineered heart valve

Dohmen, Pascal M.; Lembcke, Alexander; Hotz, Holger; Kivelitz, Dietmar; Konertz, Wolfgang

doi:10.1016/s0003-4975(02)03881-x

Cited by 143 publications

(91 citation statements)

References 5 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Our research is directed towards the use of homologous matrices, in view of unfavorable results of heterologous matrices published by Simon et al [29] and also due to the possibility of transmission of retroviruses to humans [30].…”

Section: Discussionmentioning

confidence: 99%

Avaliação do comportamento biológico de homoenxertos valvares pulmonares descelularizados: estudo experimental em ovinos

Navarro¹,

Costa²,

Mulinari³

et al. 2010

Rev Bras Cir Cardiovasc

View full text Add to dashboard Cite

Section: Discussionmentioning

confidence: 99%

Avaliação do comportamento biológico de homoenxertos valvares pulmonares descelularizados: estudo experimental em ovinos

Navarro¹,

Costa²,

Mulinari³

et al. 2010

Rev Bras Cir Cardiovasc

View full text Add to dashboard Cite

“…Diverse chemical treatments capable of decellularizing the valvar cuspids and the arterial walls of the conduits have been proposed, including the use of detergents, hypotonic and hypertonic solutions, enzymes such as RNase and DNase, ethanol and deoxycholic acid [11][12][13][14][15][16][17][18].…”

Section: Commentsmentioning

confidence: 99%

“…Curiously, reendothelialization of the grafts was not observed. DOHMEN et al [12,15] studied decellularized porcine pulmonary heterografts that were re-endothelialized in vitro, which they called pulmonary autoxenografts. When implanted in juvenile sheep, these grafts also demonstrated normal hemodynamic performance, without calcification and without the growth of pannus within the conduit.…”

Section: Commentsmentioning

confidence: 99%

“…Additionally, it is known that endothelial cells have a capacity of migrating to the inside of the matrix and to transform in fibroblastic interstitial cells, which facilitates re-population of the graft. On the other hand, in vitro seeding of endothelial cells is a much more complicated and onerous process with obvious implications in the daily routine [10,12,16].…”

Section: Commentsmentioning

confidence: 99%

“…More recent researches aim at, by means of tissue engineering, developing valves that incorporate autologous cells capable of making repairs and remodeling of the extracellular matrix, thus presenting with regenerative and growth capabilities, with the potential of greater durability [10][11][12][13][14][15][16][17][18].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

<![CDATA[<b>Experimental study with deccelularized porcine heterografts</b>: <b>the prosthesis of the future</b>]]>

Costa¹,

Dohmen²,

Lopes³

et al. 2004

Braz. j. cardiovasc. surg.

View full text Add to dashboard Cite

Objective: To make a comparative evaluation of the behavior of cryopreserved homografts and decellularized porcine heterografts implanted in the right ventricular outflow tract of juvenile sheep as well as to report on the initial clinical experience with decellularized pulmonary homografts in patients submitted to the Ross procedure.Method: In group A, four cryopreserved homografts were implanted and in group B four heterografts decellularized with deoxycholic acid were used. In each group, two animals were sacrificed at 90 days and the other two at 150 days. The explants were evaluated macroscopically, and radiologically, as well as undergoing a histological examination with HE, Gomori and Sirius Red staining. We also report our initial clinical experience with decellularized pulmonary homografts in four patients submitted to the Ross procedure.Results: All the animals survived. Echocardiograms evidenced that all the implanted valves had normal function. Although the cusps had normal appearance in both groups, calcium content was higher in the cryopreserved valves, radiologically there were traces of calcification in the cryopreserved valves but no traces were observed in the decellularized valves. Cryopreserved homografts lost their cellularity while decellularized heterografts were progressively repopulated by fibroblasts and were partially re-endothelialized. Four patients submitted to a Ross operation with decellularized homografts had a good clinical outcome with normal function in their right-sided homografts.Conclusions: Decellularized heterografts were progressively repopulated by autologous cells and exhibited minimal tendency to calcification in this model. These results suggest that decellularized heterografts may have some regenerative capacity and thus potentially greater durability than conventional cryopreserved homografts.Descriptors: Homografts. Tissue engineering. Heart valve, surgery. 75DA COSTA, FDA ET AL -Experimental study with deccelularized porcine heterografts -the prosthesis of the future Rev

show abstract

Angiogenic response induced by acellular aortic matrix in vivo

et al. 2004

View full text Add to dashboard Cite

In this study, we investigated the angiogenic response induced by acellular aortic matrices implanted in vivo onto the chick embryo chorioallantoic membrane (CAM), a useful model for such investigation. Results showed that acellular matrices were able to induce a strong angiogenic response comparable to that of fibroblast growth factor 2 (FGF-2), a wellknown angiogenic cytokine. The angiogenic response was further increased when exogenous FGF-2 or transforming growth factor beta 1 (TGF-␤1) were added to the matrices and inhibited by the addition of an anti-FGF-2 or anti-TGF-␤1 antibodies. The response may be considered dependent on a direct angiogenic effect exerted by the matrices and in part also by the presence of FGF-2 and TGF-␤1 in the acellular matrices.

show abstract

Ross operation with a tissue-engineered heart valve

Cited by 143 publications

References 5 publications

Avaliação do comportamento biológico de homoenxertos valvares pulmonares descelularizados: estudo experimental em ovinos

Avaliação do comportamento biológico de homoenxertos valvares pulmonares descelularizados: estudo experimental em ovinos

<![CDATA[<b>Experimental study with deccelularized porcine heterografts</b>: <b>the prosthesis of the future</b>]]>

Angiogenic response induced by acellular aortic matrix in vivo

Contact Info

Product

Resources

About