Rosmarinic acid exerts an antiosteoporotic effect in the RANKL-induced mouse model of bone loss by promotion of osteoblastic differentiation and inhibition of osteoclastic differentiation

Lee, Ji‐Won; Asai, Midori; Jeon, Sang‐Kyung; Iimura, Tadahiro; Yonezawa, Takayuki; Cha, Byung‐Yoon; Woo, Je‐Tae; Yamaguchi, Akira

doi:10.1002/mnfr.201400164

Cited by 30 publications

(16 citation statements)

References 64 publications

(76 reference statements)

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“…As a result, eleven bioactive compounds (rosmarinic acid, citrusin C, nepetoidin B, tilianin, isoagastachoside, apigetrin, phlorizin, luteolin, diosmetin, apigenin, and acacetin-7- O -(3”- O -acetyl)-β- d -glucopyranoside) inhibited RANKL-induced osteoclast differentiation ( Figure 5 ). Similar to our results, previous studies have shown that rosmarinic acid inhibits RANKL-induced bone loss via suppression of p38-mediated osteoclast differentiation [ 44 ], and diosmetin decreases LPS-induced osteolysis via inhibition of ERK, JNK, and reactive oxygen species-mediated osteoclast formation and differentiation [ 45 ]. In addition, phlorizin, luteolin, and apigenin prevent OVX-induced postmenopausal bone loss [ 46 , 47 , 48 ].…”

Section: Resultssupporting

confidence: 92%

Water Extract of Agastache rugosa Prevents Ovariectomy-Induced Bone Loss by Inhibiting Osteoclastogenesis

Jang

Hwang

Kim

et al. 2020

Foods

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Estrogen deficiency in postmenopausal women causes homeostatic imbalance of bone, resulting in bone loss and osteoporosis. Agastache rugosa, a plant belonging to the Lamiaceae family, is an aromatic herb, and the leaves of this herb are widely used as food ingredients. Extracts of A. rugosa have various bioactivities including anti-HIV integration, anti-inflammatory, and anti-atherogenic properties. However, the beneficial effect of A. rugosa on bone has not been studied. Therefore, we investigated the effects of water extract of A. rugosa (WEAR) on osteoclast differentiation and estrogen deficiency-induced bone loss in ovariectomized (OVX) mice as an animal model for postmenopausal osteoporosis. The oral administration of WEAR remarkably improved OVX-induced trabecular bone loss and fat accumulation in the bone marrow. WEAR suppressed receptor activator of nuclear factor-κB ligand (RANKL)-induced osteoclast differentiation in osteoclast precursor cells, subsequently inhibiting resorption activity on a bone mimetic surface. WEAR inhibited the expression of cellular oncogene fos (c-Fos) and nuclear factor of activated T-cells cytoplasmic 1 (NFATc1), key osteoclastogenic transcription factors, by decreasing RANKL-induced activation of mitogen-activated protein kinases (MAPKs), and nuclear factor-κB (NF-κB) pathways. We also identified seventeen phytochemicals present in WEAR, including five phenols and twelve flavonoids, and found eleven bioactive constituents that have anti-osteoclastogenic effects. Collectively, these results suggest that WEAR could be used to treat and prevent postmenopausal osteoporosis by suppressing osteoclastogenesis.

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Section: Resultssupporting

confidence: 92%

Water Extract of Agastache rugosa Prevents Ovariectomy-Induced Bone Loss by Inhibiting Osteoclastogenesis

Jang

Hwang

Kim

et al. 2020

Foods

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“…This finding is consistent with the fact that SM extract is used to mitigate renal oxidative, inflammatory, and glycative stresses, as well as to reduce cytokine productions. In addition, ellagic acid (Papoutsi and others ), which has potent antiinflammatory activity, ferulic acid, which possesses strong anti‐oxidative activity (Itagaki and others ), and Rosmarinic acid, which is thought to have anti‐osteoporotic effect (Lee and others ), are all enriched in SM extracts. Considering that oxidative stress and inflammation are 2 major factors involved in the pathogenesis of osteoporosis, the rich content of antioxidants and anti‐inflammatory compounds in SM extracts should contribute to its use as an anti‐osteoporotic agent.…”

Section: Resultsmentioning

confidence: 99%

Solanum Muricatum Promotes Osteogenic Differentiation of Rat Bone Marrow Stromal Cells

Wang

Gao

et al. 2017

Journal of Food Science

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Solanum muricatum (SM), also known as pepino, is known for its antioxidative and anti-inflammatory effects. The aim of this study was to evaluate the effects of SM extract in promoting osteogenic differentiation and regulating the Wnt and bone morphogenetic protein (BMP) signaling pathways. Ingredients of pepino were extracted and identified. SM extracts were used to treat rat bone marrow stromal cells (BMSCs), followed by evaluating alkaline phosphatase activities and mineralization levels. The mRNA levels of osteogenic biomarkers, including OPN and Collagen I, were also evaluated with real-time polymerase chain reaction. After treatment with SM extracts, the expressions of key proteins in the Wnt and BMP signaling pathways were assessed. DKK-1 and noggin, which are Wnt and BMP inhibitors, respectively, were added with SM extracts to investigate the role of Wnt and BMP pathways in the ameliorating effects of SM extract in osteogenesis. Treatment of BMSCs with SM extract promoted osteogenesis. Meanwhile, upregulations in the Wnt and BMP pathways were also observed. However, inhibiting both pathways compromised the effects of SM extract in promoting osteogenic differentiation. SM extract promotes osteogenic differentiation in BMSCs via promoting the Wnt and BMP signaling pathways.

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“…Overall, several bioactive compounds can potentially inhibit bone resorption by acting on osteoclasts which are the only cells capable of this role (25,29,30,(44)(45)(46). It is plausible that such mechanism plays an important role in managing feline idiopathic hypercalcemia.…”

Section: Discussionmentioning

confidence: 99%

“…Several nutrients present in chia seeds, such as omega-3 fatty acids, soluble and insoluble fibers, polyphenols, and flavonoids, can affect calcium metabolism (25,29,30,(44)(45)(46). It is reasonable to say that normalization of ionized calcium concentrations in feline idiopathic hypercalcemia is ascribable not to one single nutrient, but more seemingly to the combined effects of all these bioactive compounds.…”

Section: Discussionmentioning

confidence: 99%

“…Plant polyphenols have positive effects in reducing oxidative stress, which acts by enhancing bone resorption, differentiation, and activity of osteoclasts ( 44 ). For example, rosmarinic acid, which is one of the most represented polyphenols in chia seeds (927 μg/100 g) has proven effects on inhibiting osteoclastic differentiation ( 45 ). Similar mechanisms can also be promoted by flavonoids, which act on cell signaling pathways influencing osteoclastic differentiation, therefore inhibiting bone resorption ( 46 ).…”

Section: Discussionmentioning

confidence: 99%

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Managing Feline Idiopathic Hypercalcemia With Chia Seeds (Salvia hispanica L.): A Case Series

Fantinati

Priymenko

2020

Front. Vet. Sci.

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Background: We describe for the first time the use of chia seeds (Salvia hispanica L.) as a non-pharmacological solution in managing feline idiopathic hypercalcemia when dietary change alone fails. Case Summary: Over a 2-year period of time, three female spayed, middle-aged, Domestic Shorthair cats were diagnosed with idiopathic hypercalcemia. Reason for consultation were lethargy and dysorexia, with a single episode of vomiting described in one cat and dysuria in another. Thorough diagnostic work-up included complete blood count, serum biochemistry, urinalysis, ionized calcium, calcemic hormones, parathyroid hormone-related protein, and imaging of chest and abdomen. Based on different nutritional reasons, each cat was switched to a different high-moisture pet food as first-step in managing the disorder: a high-fiber diet, a diet formulated for chronic kidney disease management and a diet designed to prevent calcium oxalate urolithiasis. In the three cats, 6 weeks of dietary change alone did not result in normocalcemia. Before resorting to any pharmacological solution, supplementation to the diet of chia seeds (2 g/cat/day) was started. After 4 weeks from the introduction of Salvia hispanica L., all cats achieved normalization of ionized calcium concentration. Conclusion: Chia seeds (Salvia hispanica L.) supplementation could be a useful tool in managing feline idiopathic hypercalcemia.

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Rosmarinic acid exerts an antiosteoporotic effect in the RANKL-induced mouse model of bone loss by promotion of osteoblastic differentiation and inhibition of osteoclastic differentiation

Abstract: Rosmarinic acid has dual regulatory effects on bone metabolism and may control the bone functions by controlling osteoblastic and osteoclastic differentiation.

Cited by 30 publications

References 64 publications

Water Extract of Agastache rugosa Prevents Ovariectomy-Induced Bone Loss by Inhibiting Osteoclastogenesis

Water Extract of Agastache rugosa Prevents Ovariectomy-Induced Bone Loss by Inhibiting Osteoclastogenesis

Solanum Muricatum Promotes Osteogenic Differentiation of Rat Bone Marrow Stromal Cells

Managing Feline Idiopathic Hypercalcemia With Chia Seeds (Salvia hispanica L.): A Case Series

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