Roseovarius carneus sp. nov., a novel bacterium isolated from a coastal phytoplankton bloom in Xiamen

Abstract

A Gram-stain-negative, non-motile, ovoid or short rod shaped and aerobic marine bacterium, designated as strain LXJ103T, was isolated from a coastal phytoplankton bloom in Xiamen, PR China. Cells were oxidase- and catalase-positive. Strain LXJ103T grew at 4–40 °C (optimum, 28–37 °C), at pH 6–10 (optimum, pH 8.5) and with 1–15 % (w/v) NaCl (optimum, 3 %). The major cellular fatty acids (>10 %) were iso-C18 : 1 ω7c/iso-C18 : 1 ω6c (70.2 %) and C16 : 0 (10.3 %). The following polar lipids were found to be present: phosphatidylglycerol, phosphatidylethanolamine, two unidentified phospholipids and five unknown glycolipids. The predominant respiratory quinone was ubiquinone-10. Strain LXJ103T exhibited the highest 16S rRNA gene sequence similarity to Roseovarius litorisediminis D1-W8T (96.97 %). The phylogenetic trees based on 16S rRNA gene sequences showed that strain LXJ103T was a member of the genus Roseovarius . The draft genome size of strain LXJ103T is 3.05 Mb with a genomic G+C content of 61.22 mol%. The digital DNA–DNA genome hybridization value of strain LXJ103T compared with the most similar type strain R. litorisediminis CECT 8287T was 18.80 %. The average nucleotide identity value between strain LXJ103T and R. litorisediminis CECT 8287T was 72.60 %. On the basis of polyphasic data, strain LXJ103T represents a novel species of the genus Roseovarius , for which the name Roseovarius carneus sp. nov. is proposed. The type strain is LXJ103T (=CGMCC 1.19168T=MCCC 1K06527T=JCM 34778T).

Roseovarius pelagicus sp. nov., a facultatively anaerobic bacterium with potential for degrading polypropylene, isolated from Arctic seawater

“…The major polar lipids phosphatidylcholine and phosphatidylglycerol were present in strain HL-MP18 T and the related Roseovarius species (Table 1), a feature that is generally found in the genus Roseovarius [67]. The isoprenoid quinone of strain HL-MP18 T was ubiquinone-10, which is also common in the genus Roseovarius [1,4,[8][9][10][11][12][13][14][25][26][27][28][29][30][31] In conclusion, the phylogenetic, genomic, phenotypic and chemotaxonomic data obtained in the present study suggest that strain HL-MP18 T should be placed in the genus Roseovarius as a representative of a novel species, for which the name Roseovarius pelagicus is proposed.…”

“…For characterizing the phenotypic, biochemical and chemotaxonomic properties of strain HL-MP18 T , R. carneus JCM 34778 T [30], R. litorisediminis KCTC 32327 T [15] and R. spongiae HN-E21 T [28] were obtained from the Japan Collection of Microorganisms (JCM), the Korean Collection for Type Cultures (KCTC) and the Marine Culture Collection of China (MCCC), respectively, to serve as reference strains. Unless otherwise stated, strain HL-MP18 T , R. carneus JCM 34778 T and R. litorisediminis KCTC 32327 T were grown on MA for 3-5 days at 25 °C, while R. spongiae HN-E21 T was grown at 35 °C.…”

“… API 20NE assay showed the congruent results. API 20E and API 20NE assays showed the congruent results. PC, phosphatidylcholine; PG, phosphatidylglycerol; PE, phosphatidylethanolamine; DPG, diphosphatidylglycerol; APL, an unidentified aminophospholipid; AL, an unidentified aminolipid; L, an unidentified lipid. Data from Sun et al . [30]. Data from Park et al .…”

“…The major polar lipids phosphatidylcholine and phosphatidylglycerol were present in strain HL-MP18 T and the related Roseovarius species (), a feature that is generally found in the genus Roseovarius [67]. The isoprenoid quinone of strain HL-MP18 T was ubiquinone-10, which is also common in the genus Roseovarius [1, 4, 8–14, 25–31]…”

“…However, the detection of bacterial sequences belonging to this genus in PP microplastics suggests their potential association with PP degradation in marine environments [6]. Most members of the genus Roseovarius have been isolated from marine environments, such as seawater [4,[7][8][9][10][11][12][13][14], sediment [15][16][17][18][19][20][21][22][23][24], soil [25,26], decayed whale bone [27], sponge [28], shell [29] and dinoflagellates [30,31]. In this study, we describe the characteristics of a marine bacterium, designated HL-MP18 T , using a polyphasic approach to identify its taxonomic position.…”