Roseococcus suduntuyensis sp. nov., a new aerobic bacteriochlorophyll a-containing bacterium isolated from a low-mineralized soda lake of Eastern Siberia

Boldareva, E. N.; Tourova, T. P.; Kolganova, T. V.; Moskalenko, A. A.; Makhneva, Z. K.; Горленко, В. М.

doi:10.1134/s0026261709010123

Cited by 24 publications

(18 citation statements)

References 8 publications

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“…The composition of the microbial community in the sediments (Gorlenko et al , 1999) and microbial mats in lake Khilganta was studied in the 1990s (Kompantseva et al , 2005); however, little is known about the planktonic bacterial community in these lakes. The presence of photosynthetic bacteria in these lakes has been indicated by the previous work of Bryantseva et al (1999), Kompantseva et al (2005, 2007) and Boldareva et al (2008, 2009a, b), who isolated a number of new species of APB from these environments. However, more detailed information about the abundance and activity of phototrophs in such environments is missing.…”

Section: Introductionmentioning

confidence: 84%

High abundances of aerobic anoxygenic phototrophs in saline steppe lakes

Medová¹,

Boldareva²,

Hrouzek³

et al. 2011

FEMS Microbiology Ecology

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We studied the distribution of anoxygenic phototrophs in 23 steppe lakes in the Transbaikal region (Russia), in Uzbekistan (Central Asia) and in the Crimean peninsula (Ukraine). The lakes varied in their mineral content and composition (salinities from 0.2 to 300 g L À1 ). The Transbaikal lakes were alkaline (pH 4 9), with high amounts of soda. The Uzbek and Crimean lakes were more pH neutral, frequently with high amounts of sulfates. The presence of anoxygenic phototrophs was registered by infrared epifluorescence microscopy, infrared fluorometry and pigment analyses. In mostly shallow, fully oxic lakes, the anoxygenic phototrophs represented 7-65% of the total prokaryotes, with the maxima observed in Transbaikal soda lakes Gorbunka (32%), Khilganta (65%), Zanday (58%) and Zun-Kholvo (46%). Some of the lakes contained over 1 mg bacteriochlorophyll L À1 .In contrast, only small amounts of anoxygenic phototrophs were present in highly mineralized lakes ( 4 100 g total salts L À1 ); Borzinskoe, Tsagan-Nur (Transbaikal), Staroe (Crimea) and in the residual part of the south-west Aral Sea (Uzbekistan). The oxic environment and the specific diurnal changes of bacteriochlorophyll concentration observed suggest that the phototrophic community was mostly composed of aerobic anoxygenic phototrophs. The high abundances and bacteriochlorophyll concentrations point to an important role of aerobic anoxygenic phototrophs in the habitats studied.

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Section: Introductionmentioning

confidence: 84%

High abundances of aerobic anoxygenic phototrophs in saline steppe lakes

Medová¹,

Boldareva²,

Hrouzek³

et al. 2011

FEMS Microbiology Ecology

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“…The results of 16S rDNA gene sequence comparison indicated that strain R-30 T showed a highest similarity value of 95.6% to Roseococcus suduntuyensis SHET T (Boldareva et al, 2009) (Yurkov et al, 1994) and 92.8% to Roseomonas pecuniae N75 T (Lopes et al, 2011). In the neighbor-joining tree ( Fig.…”

Section: Phylogenetic Analysismentioning

confidence: 94%

Sediminicoccus rosea gen. nov. sp. nov., isolated from the sediment of a eutrophic lake

et al. 2013

J. Gen. Appl. Microbiol.

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“…Pellets were either extracted with cold 100% acetone, a mixture of acetone/methanol (7:2 v/v), or with 95% cold-buffered methanol (2% ammonium acetate), always at -20°C in the dark. The mixture of acetone/methanol (7:2 v/v) was chosen because it is the most used combination in the extraction of bacterial pigments (e.g., Biebl et al 2005;Glaeser and Klug 2005;Biebl and Wagner-Dobler 2006;Koblížek et al 2006;Olson et al 2007;Boldareva et al 2009). Solvent extracts were filtered through Fluoropore PTFE filter membranes (0.2 μm pore size, Merck) to remove cell debris and immediately injected in the HPLC.…”

Section: Hplc Pigment Analysismentioning

confidence: 99%

Extraction and quantification of pigments in aerobic anoxygenic phototrophic bacteria

Ruivo

Cartaxana

Cardoso

et al. 2014

Limnology & Ocean Methods

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Using a 15-min High Performance Liquid Chromatography (HPLC) elution program, the current study compared pigment extraction efficiencies in aerobic anoxygenic phototrophic bacteria (AAPB; Dinoroseobacter shibae, Roseobacter denitrificans, Erythrobacter longus, and a strain of Rhodovulum sp. isolated from Tagus estuarine intertidal sediments -SPS1025 T ) using four extraction times (15, 30, 60, and 120 min) and three solvents (acetone, acetone/methanol, and methanol). Overall, increasing extraction time showed no significant effect on bacteriochlorophyll a (BChl a) and carotenoid extraction efficiencies in all strains. BChl a extraction efficiency was significantly higher in the mixture acetone/methanol and methanol, but methanol induced significantly higher BChl a degradation for all strains. Carotenoid extraction efficiencies were dependent on solvent and pigment polarity. Non-polar carotenoids were better extracted with acetone and polar carotenoids with methanol. We suggest the application of the used HPLC procedure for the quantification of photosynthetic pigments in AAPB due to its high sample throughput and peak resolution. We also recommend the use acetone/methanol for extraction of AAPB photosynthetic pigments with extraction periods of 15 min to achieve higher BChl a extraction yields and minimize carotenoid degradation.

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Roseococcus suduntuyensis sp. nov., a new aerobic bacteriochlorophyll a-containing bacterium isolated from a low-mineralized soda lake of Eastern Siberia

Cited by 24 publications

References 8 publications

High abundances of aerobic anoxygenic phototrophs in saline steppe lakes

High abundances of aerobic anoxygenic phototrophs in saline steppe lakes

Sediminicoccus rosea gen. nov. sp. nov., isolated from the sediment of a eutrophic lake

Extraction and quantification of pigments in aerobic anoxygenic phototrophic bacteria

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