Rosa1, a Transposable Element-Like Insertion, Produces Red Petal Coloration in Rose Through Altering RcMYB114 Transcription

Li, Maofu; Zhang, Hui; Yang, Yuan; Wang, Hua; Xue, Zhen; Fan, Youwei; Sun, Peng; Zhang, Hong; Zhang, Xinzhu; Jin, Wanmei

doi:10.3389/fpls.2022.857684

Cited by 8 publications

(10 citation statements)

References 81 publications

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“…Primer design based on the complete coding sequence (CDS) of HG_GLEAN_10010973 was obtained by RACE ( Supplementary Table S1 ). The target fragment without the stop codon was amplified by PCR and cloned into the modified vector pBI221-EGFP ( Li et al., 2012 ; Li et al., 2022 ). The 35S::GFP and 35S::GFP- LsAPRR2 fusion vectors were transformed into Agrobacterium tumefaciens GV3101 strain.…”

Section: Methodsmentioning

confidence: 99%

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Forward genetic studies reveal LsAPRR2 as a key gene in regulating the green color of pericarp in bottle gourd (Lagenaria siceraria)

Huo

Gui²,

Yu³

et al. 2023

Front. Plant Sci.

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The fruit peel color is an important factor that affects its quality. However, genes involved in regulating pericarp color in bottle gourd (Lagenaria siceraria) have not been explored to date. Genetic analysis of color traits in bottle gourd peel through a genetic population of six generations demonstrated that the green color of peels is inherited as a single gene dominant trait. Combined phenotype-genotype analysis of recombinant plants using BSA-seq mapped the candidate gene to a 22.645 Kb interval at the head end of chromosome 1. We observed that the final interval contained only one gene, LsAPRR2 (HG_GLEAN_10010973). Sequence and spatiotemporal expression analyses of LsAPRR2 unraveled two nonsynonymous mutations (A→G) and (G→C) in the parental CDS sequences. Further, LsAPRR2 expression was higher in all green-skinned bottle gourds (H16) at various stages of fruit development than in white-skinned bottle gourds (H06). Cloning and sequence comparison of the two parental LsAPRR2 promoter regions indicated 11 bases insertion and 8 SNPs mutations in the region -991~-1033, upstream of the start codon in white bottle gourd. Proof of GUS reporting system, Genetic variation in this fragment significantly reduced the expression of LsAPRR2 in the pericarp of white bottle gourd. In addition, we developed a tightly linked (accuracy 93.88%) InDel marker for the promoter variant segment. Overall, the current study provides a theoretical basis for comprehensive elucidation of the regulatory mechanisms underlying the determination of bottle gourd pericarp color. This would further help in the directed molecular design breeding of bottle gourd pericarp.

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Section: Methodsmentioning

confidence: 99%

“…The first 2000 bp of the biparental LsAPRR2 promoter were cloned ( Supplementary Table S1 ) and subsequently ligated into an expression vector (pBI121) containing a β-Glucuronidase (GUS) reporter gene ( Li et al., 2022 ). The Agrobacterium tumefaciens GV3101 strain was transformed with the fusion reporter vectors as well as the empty vector.…”

Section: Methodsmentioning

confidence: 99%

Forward genetic studies reveal LsAPRR2 as a key gene in regulating the green color of pericarp in bottle gourd (Lagenaria siceraria)

Huo

Gui²,

Yu³

et al. 2023

Front. Plant Sci.

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“…Few MYB TFs regulating anthocyanin synthesis were identified from Rosaceae ornamental plants, such as RcMYB114 in Rosa sp. and PpMYB108 in peach flower (Li et al, 2022;Khan et al, 2022).…”

Section: Key Candidate Tfs Involved In Anthocyanin Synthesis In R Rug...mentioning

confidence: 99%

Transcriptome and chemical analyses revealed the mechanism of flower color formation in Rosa rugosa

Wang

Li²,

Zhu³

et al. 2022

Front. Plant Sci.

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Rosa rugosa is a famous Chinese traditional flower with high ornamental value and well environmental adapt ability. The cultivation of new colorful germplasms to improve monotonous flower color could promote its landscape application. However, the mechanism of flower color formation in R. rugosa remains unclear. In this study, combined analyses of the chemical and transcriptome were performed in the R. rugosa germplasms with representative flower colors. Among the identified anthocyanins, cyanidin 3,5-O-diglucoside (Cy3G5G) and peonidin 3,5-O-diglucoside (Pn3G5G) were the two dominant anthocyanins in the petals of R. rugosa. The sum content of Cy3G5G and Pn3G5G was responsible for the petal color intensity, such as pink or purple, light- or dark- red. The ratio of Cy3G5G to Pn3G5G was contributed to the petal color hue, that is, red or pink/purple. Maintaining both high relative and high absolute content of Cy3G5G may be the precondition for forming red-colored petals in R. rugosa. Cyanidin biosynthesis shunt was the dominant pathway for anthocyanin accumulation in R. rugosa, which may be the key reason for the presence of monotonous petal color in R. rugosa, mainly pink/purple. In the upstream pathway of cyanidin biosynthesis, 35 differentially expressed structural genes encoding 12 enzymes co-expressed to regulate the sum contents of Cy3G5G and Pn3G5G, and then determined the color intensity of petals. RrAOMT, involved in the downstream pathway of cyanidin biosynthesis, regulated the ratio of Cy3G5G to Pn3G5G via methylation and then determined the color hue of petals. It was worth mentioning that significantly higher delphinidin-3,5-O-diglucoside content and RrF3’5’H expression were detected from deep purple-red-flowered 8-16 germplasm with somewhat unique and visible blue hue. Three candidate key transcription factors identified by correlation analysis, RrMYB108, RrC1, and RrMYB114, might play critical roles in the control of petal color by regulating the expression of both RrAOMT and other multiple structural genes. These results provided novel insights into anthocyanin accumulation and flower coloration mechanism in R. rugosa, and the candidate key genes involved in anthocyanin biosynthesis could be valuable resources for the breeding of ornamental plants in future.

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“…Previous analysis of allele-specific expression in individuals heterozygous for the light and dark flower color allele demonstrated that natural variation in PdMyb1 expression is associated with cis-regulatory mutation(s) (Hopkins & Rausher, 2011). Cis-regulatory polymorphisms causing functional variation in the expression of R2R3-Myb transcription factors are often within 5kb of the gene (Sheehan et al, 2016;Li et al, 2022). Therefore, we hypothesized that expression differences in PdMyb1 are due to proximal mutations.…”

Section: Point Mutations At Pdmyb1 Are Associated With Natural Variat...mentioning

confidence: 99%

“…Neither of these motifs were predicted with the SNP1 light allele "G". CAAT-boxes in antisense and "CCAAA" CCAAT-box derivatives can enhance transcription activation in plant promoters (Tiwari et al, 2010), and the presence\of an E2F TF binding site-like sequence in an R2R3-Myb promoter has been implicated in increased gene expression and flower color in roses (Li et al, 2022). The dark-associated allele at SNP2 ("A") converts the binding site of a putative HD-Zip class I TF "CCAATGATTG" motif to that of a HD-Zip class II TF "CCAATAATTG" (Figure 3d), and this base change also modifies the DNA base following a CAAT-Box sequence "CCAATA".…”

Section: Dark Associated Mutations Evolve New Cis-regulatory Motifs I...mentioning

confidence: 99%

Acis-regulatory point mutation at a R2R3-Myb transcription factor contributes to speciation by reinforcement inPhlox drummondii

Garner¹,

Cameron²,

Berardi³

et al. 2023

Preprint

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The process of reinforcement, whereby selection favors the evolution of increased reproductive trait divergence to reduce costly hybridization between species, has been well documented in nature, yet we know very little about how this process evolves at the molecular level. In this study, we combine functional characterization and genetic association tests to identify the mutational basis of reinforcement in the Texas wildflower Phlox drummondii. P. drummondii evolved from light to dark flower color intensity by selection to stop hybridization with the closely related species P. cuspidata, and previous research suggests differential expression of a R2R3-Myb transcription factor underlies this phenotypic transition. Using gene-silencing experiments, we demonstrate expression of this transcription factor does control variation in flower color intensity. We then apply association mapping across a large genomic region flanking the R2R3-Myb gene and identified a point mutation within the gene's promoter that is highly associated with flower color intensity in nature. Alleles at this mutation site match the expected patterns of dominance, create variation in predicted cis-regulatory motifs within the R2R3-Myb proximal promoter, and occur in the direction of evolution predicted for flower color variation in this system. By identifying the mutational basis of reinforcement in this system we demonstrate that, as predicted by theory, reproductive isolation can evolve despite gene flow through a very simple genetic basis.

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Rosa1, a Transposable Element-Like Insertion, Produces Red Petal Coloration in Rose Through Altering RcMYB114 Transcription

Cited by 8 publications

References 81 publications

Forward genetic studies reveal LsAPRR2 as a key gene in regulating the green color of pericarp in bottle gourd (Lagenaria siceraria)

Forward genetic studies reveal LsAPRR2 as a key gene in regulating the green color of pericarp in bottle gourd (Lagenaria siceraria)

Transcriptome and chemical analyses revealed the mechanism of flower color formation in Rosa rugosa

Acis-regulatory point mutation at a R2R3-Myb transcription factor contributes to speciation by reinforcement inPhlox drummondii

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