ROS production and lipid catabolism in desiccating Shorea robusta seeds during aging

Parkhey, Suruchi; Naithani, S. C.; Keshavkant, S.

doi:10.1016/j.plaphy.2012.06.008

Cited by 97 publications

(65 citation statements)

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“…Dehydration and rehydration during seed development or during germination are associated with high levels of oxidative stress (Dandoy et al, 1987;Rajjou et al, 2012). Overaccumulation of reactive oxygen species (ROS) can cause oxidative damage to a wide range of cellular components and cause DNA damage, reducing the seed's ability to germinate (Bailly et al, 2008;Chen et al, 2011;Parkhey et al, 2012). An optimal range of ROS levels is required for successful germination (Bailly et al, 2008).…”

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confidence: 99%

ASCORBATE PEROXIDASE6 Protects Arabidopsis Desiccating and Germinating Seeds from Stress and Mediates Cross Talk between Reactive Oxygen Species, Abscisic Acid, and Auxin

et al. 2014

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A seed's ability to properly germinate largely depends on its oxidative poise. The level of reactive oxygen species (ROS) in Arabidopsis (Arabidopsis thaliana) is controlled by a large gene network, which includes the gene coding for the hydrogen peroxide-scavenging enzyme, cytosolic ASCORBATE PEROXIDASE6 (APX6), yet its specific function has remained unknown. In this study, we show that seeds lacking APX6 accumulate higher levels of ROS, exhibit increased oxidative damage, and display reduced germination on soil under control conditions and that these effects are further exacerbated under osmotic, salt, or heat stress. In addition, ripening APX6-deficient seeds exposed to heat stress displayed reduced germination vigor. This, together with the increased abundance of APX6 during late stages of maturation, indicates that APX6 activity is critical for the maturation-drying phase. Metabolic profiling revealed an altered activity of the tricarboxylic acid cycle, changes in amino acid levels, and elevated metabolism of abscisic acid (ABA) and auxin in drying apx6 mutant seeds. Further germination assays showed an impaired response of the apx6 mutants to ABA and to indole-3-acetic acid. Relative suppression of abscisic acid insensitive3 (ABI3) and ABI5 expression, two of the major ABA signaling downstream components controlling dormancy, suggested that an alternative signaling route inhibiting germination was activated. Thus, our study uncovered a new role for APX6, in protecting mature desiccating and germinating seeds from excessive oxidative damage, and suggested that APX6 modulate the ROS signal cross talk with hormone signals to properly execute the germination program in Arabidopsis.

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confidence: 99%

ASCORBATE PEROXIDASE6 Protects Arabidopsis Desiccating and Germinating Seeds from Stress and Mediates Cross Talk between Reactive Oxygen Species, Abscisic Acid, and Auxin

et al. 2014

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“…Over-accumulation of reactive oxygen species (ROS) can cause oxidative damage to a wide range of cellular components, including DNA, proteins and lipids, and reduce seeds ability to germinate. [1][2][3] An optimal range of ROS levels, termed the 'oxidative window of germination', is required for successful germination; 2 too little ROS will result in suppressed germination (e.g., in dormant seeds) and too much ROS will lead to delaying or inhibiting germination due to accumulation oxidative damage. 2 A tight regulation is therefore required to balance ROS production and scavenging and maintain the cellular redox poise.…”

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New cross talk between ROS, ABA and auxin controlling seed maturation and germination unraveled in APX6 deficient Arabidopsis seeds

Chen

Twito

Miller

2014

Plant Signaling & Behavior

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“…However, it remains to be explored fully whether oxidative injury is the cause or consequence of the tissue damage or viability loss (Hendry et al 1992). Desiccation promoted ROS accumulation linked loss of viability has already been documented in several recalcitrant species like Shorea robusta (Chaitanya and Naithani 1994;Parkhey et al 2012), Acer saccharinum (Pukacka and Ratajczak 2006) and Antiaris toxicaria (Cheng and Song 2008) as well as in orthodox species; Helianthus annuus (Bailly 2004) and Glycine max (Liu et al 2008). A wide variety of ROS are known, out of which three [hydroxyl radical, hydrogen peroxide and superoxide ( .…”

Section: Introductionmentioning

confidence: 98%

“…It has already been suggested that during desiccation, electron transport chain of mitochondrial and microsomal membranes becomes disrupted, which results in reactive oxygen species (ROS)-induced oxidative situation that culminates with loss of seed viability (Parkhey et al 2012;2014a). Moreover, Song and co-workers (2009) recently reported a fall in the activity of cytochrome c oxidase in desiccating radicles and embryos of Antaris toxicaria and Zea mays respectively, allowing excessive accumulation of ROS in these tissues.…”

Section: Introductionmentioning

confidence: 99%

“…Lipid peroxidation was shown to be one of the most common deleterious events, resulting oxidation of polyunsaturated fatty acids, thereby releasing several cytotoxic by-products that includes malondialdehyde (MDA), alkoxyl radical, epoxides and alkanes (Parkhey et al 2012). Keshavkant et al (2013) reported that lipid peroxidation is one of the well-established and foremost mechanisms of cellular injury in both plant and animal cells.…”

Section: Introductionmentioning

confidence: 99%

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Increased rate of drying reduces metabolic inequity and critical water content in radicles of Cicer arietinum L.

Chandra

Tandon

Keshavkant

2015

Physiol Mol Biol Plants

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Orthodox seed serves as easily accessible model to study desiccation-sensitivity in plant tissues because once they undergo germination, they become sensitive to desiccation imposed injuries. In the proposed study, effects of rate of drying on the viability, electrolyte leakage, superoxide accumulation, lipid-protein oxidation and antioxidant enzymes were explored in excised radicles of Cicer arietinum L. under dehydration and wet storage. For both the drying conditions, desiccation could be explained by exponential and inverse functions. Under rapid drying tissue viability as scored by germination efficiency and tetrazolium staining remained 100 % all through the analysis (24 h) but declined remarkably after 0.30 g g(-1) fresh mass water content (4 days) under slow drying. Moreover, precipitous fall in tissue viability was observed after 2 weeks of wet storage. Rapid drying was also accompanied with limited amounts of electrolyte leakage, superoxide radical, malondialdehyde and protein hydroperoxide, together with enhanced level of protein. Additionally, activities of both superoxide dismutase and ascorbate peroxidase were increased in rapidly dried radicles, but guaiacol peroxidase was declined. In contrary, above referred biomarkers were observed to perform either inversely or poorly during slow drying and wet storage suggesting that above documented alterations might be the resultant of ageing and not desiccation. Gathered data demonstrated that increased drying lowers the critical water content for tissue survival and also reduces the risk of damage resulting from aqueous-based deleterious reactions. Additionally, it also showed that growing radicles are a popular model to explore desiccation-sensitivity in plant tissues and/or seeds.

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ROS production and lipid catabolism in desiccating Shorea robusta seeds during aging

Cited by 97 publications

References 30 publications

ASCORBATE PEROXIDASE6 Protects Arabidopsis Desiccating and Germinating Seeds from Stress and Mediates Cross Talk between Reactive Oxygen Species, Abscisic Acid, and Auxin

ASCORBATE PEROXIDASE6 Protects Arabidopsis Desiccating and Germinating Seeds from Stress and Mediates Cross Talk between Reactive Oxygen Species, Abscisic Acid, and Auxin

New cross talk between ROS, ABA and auxin controlling seed maturation and germination unraveled in APX6 deficient Arabidopsis seeds

Increased rate of drying reduces metabolic inequity and critical water content in radicles of Cicer arietinum L.

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